Molecular analyses of genetic variation and phylogenetic relationship in Indian soap nut ( Sapindus L.) and closely related taxa of the family Sapindaceae

Meta Gene ◽  
2017 ◽  
Vol 13 ◽  
pp. 50-56 ◽  
Author(s):  
Kamalesh S. Mahar ◽  
Lok Man S. Palni ◽  
Shirish A. Ranade ◽  
Veena Pande ◽  
Tikam S. Rana
2017 ◽  
Vol 124 (3) ◽  
pp. 201-214 ◽  
Author(s):  
R Abdel-Gaber ◽  
F Abdel-Ghaffar ◽  
S Maher ◽  
AM El-Mallah ◽  
S Al Quraishy ◽  
...  

Pathogens ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 41
Author(s):  
Marcos Godoy ◽  
Daniel A. Medina ◽  
Rudy Suarez ◽  
Sandro Valenzuela ◽  
Jaime Romero ◽  
...  

Piscine orthoreovirus (PRV) belongs to the family Reoviridae and has been described mainly in association with salmonid infections. The genome of PRV consists of about 23,600 bp, with 10 segments of double-stranded RNA, classified as small (S1 to S4), medium (M1, M2 and M3) and large (L1, L2 and L3); these range approximately from 1000 bp (segment S4) to 4000 bp (segment L1). How the genetic variation among PRV strains affects the virulence for salmonids is still poorly understood. The aim of this study was to describe the molecular phylogeny of PRV based on an extensive sequence analysis of the S1 and M2 segments of PRV available in the GenBank database to date (May 2020). The analysis was extended to include new PRV sequences for S1 and M2 segments. In addition, subgenotype classifications were assigned to previously published unclassified sequences. It was concluded that the phylogenetic trees are consistent with the original classification using the PRV genomic segment S1, which differentiates PRV into two major genotypes, I and II, and each of these into two subgenotypes, designated as Ia and Ib, and IIa and IIb, respectively. Moreover, some clusters of country- and host-specific PRV subgenotypes were observed in the subset of sequences used. This work strengthens the subgenotype classification of PRV based on the S1 segment and can be used to enhance research on the virulence of PRV.


2012 ◽  
Vol 23 (2) ◽  
pp. 53-61 ◽  
Author(s):  
Wei He ◽  
Wei-Hua Lu ◽  
Xi-Guo Li ◽  
Ning-Ning Lu ◽  
Dong-Fang Sun ◽  
...  

Zootaxa ◽  
2003 ◽  
Vol 355 (1) ◽  
pp. 1 ◽  
Author(s):  
WILLIAM P. LEONARD ◽  
LYLE CHICHESTER ◽  
JIM BAUGH ◽  
THOMAS WILKE

A new genus and species of arionid slug, Kootenaia burkei n. gen. et n. sp., are formally described from northern Idaho, United States. This taxonomic decision is based on comparative anatomical and molecular data involving representatives of a total of ten species and three additional genera (Hemphillia, Prophysaon, and Zacoleus) of the family Arionidae. The anatomical analyses show that the new genus is characterized by a major autapomorphy, the complete absence of an epiphallus, which is found in all other arionids. The molecular analyses using two mitochondrial genes and the anatomical data produce congruent topologies. Overall, there is a high degree of concordance between the anatomical and molecular datasets.


Zootaxa ◽  
2009 ◽  
Vol 2293 (1) ◽  
pp. 35-52 ◽  
Author(s):  
RACHAEL A. KING

Amphipods within the family Chiltoniidae are an abundant yet taxonomically poorly known member of Australian freshwater habitats. With only four species known from Australia, the group is inadequately defined and marked by taxonomists as difficult to identify. Recent molecular analyses of chiltoniids from mound springs in South Australia detected several distinct species, prompting a morphological revision of material from the central and southern Lake Eyre region. Clear groups defined by unique combinations of morphological characters (focusing on uropodal, coxal, male gnathopod 2, and antennal morphology) were found that closely correlated with clades found in the molecular analyses. Arabunnachiltonia n. gen. is established for A. murphyi n. sp. from Strangways Springs in South Australia. Wangiannachiltonia n. gen. is established for W. guzikae n. sp. from Davenport Springs in South Australia. The chiltoniid genera are discussed and a key is presented to the known Australian species.


Zootaxa ◽  
2020 ◽  
Vol 4858 (4) ◽  
pp. 521-541
Author(s):  
SERGEY G. SOKOLOV ◽  
ALEXANDER P. KALMYKOV ◽  
SVETLANA V. MALYSHEVA

Sets of small ribosomal DNA (SSU rDNA) and large ribosomal DNA (LSU rDNA) sequences were obtained for Philometroides moraveci Vismanis & Yunchis, 1994, Philometra kotlani (Molnár, 1969), Philometra rischta Skrjabin, 1923, Philometra cf. obturans (Prenant, 1886) (Philometridae), Sinoichthyonema amuri (Garkavi, 1972), Agrachanus scardinii (Molnár, 1966), Kalmanmolnaria intestinalis (Dogiel & Bychowsky, 1934) and Skrjabillanus tincae Shigin & Shigina, 1958 (Skrjabillanidae). Phylogenetic analysis of SSU rDNA data shows that dracunculoid nematodes are divided into two well-supported clades designated as Clade I and Clade II, respectively. Clade I includes the type species of the genus Philonema Kuitunen-Ekbaum, 1933, some species from the family Daniconematidae Moravec & Køie, 1987 and two subfamilies of skrjabillanids, Skrjabillaninae Shigin & Shigina, 1958 and Esocineminae Moravec, 2006. Clade II unites species from the families Dracunculidae Stiles, 1907, Micropleuridae Baylis & Daubney, 1926 and Philometridae Baylis & Daubney, 1926. Within the Philometridae, there are several well-supported groups of species, one of which unites freshwater Philometra spp. from the Palearctic cyprinids, identified as P. kotlani, P rischta, P. ovata (Zeder, 1803) and P. cyprinirutili (Creplin, 1825). However, the phylogenetic relationships of most philometrids are unresolved. An analysis of partial SSU and LSU rDNA sequences indicates that there is no direct phylogenetic relationship between Agrachanus Tikhomirova, 1971 (type species Skrjabillanus scardinii Molnár, 1966) and Skrjabillanus Shigin & Shigina, 1958 (type species Sk. tincae), which means that the genus Agrachanus can be resurrected. Our study confirms that Philonematinae Ivashkin, Sobolev & Khromova, 1971 should be elevated to the family rank. We formally establish the family Philonematidae Ivashkin, Sobolev & Khromova, 1971 stat. nov. We also suggest combining the superfamilies Dracunculoidea Stiles, 1907 and Camallanoidea Railliet & Henry, 1915 into the infraorder Camallanomorpha Roberts, Janovy & Nadler, 2013. 


Parasitology ◽  
2016 ◽  
Vol 143 (12) ◽  
pp. 1592-1604 ◽  
Author(s):  
PETR HENEBERG ◽  
JILJÍ SITKO ◽  
JIŘÍ BIZOS ◽  
ELIZABETH C. HORNE

SUMMARYThe Renicolidae are digenean parasites of piscivorous and molluscivorous birds. Although they exhibit few morphological autapomorphies and are highly variable, the numerous suggested re-classifications within the family have never been supported by any molecular analyses. We address the possible synonymization of species within theRenicola pinguiscomplex suggested previously by Odening. We provide and analyse sequences of two nuclear (ITS2, 28S rDNA) and two mitochondrial (CO1, ND1) DNA loci of central European species of the Renicolidae, namelyRenicola lari, Renicola pinguisandRenicola sternaesp. n., and we also provide first sequences ofRenicola sloanei. The combined molecular and comparative morphological analysis confirms the previously questioned validity of the threeRenicolaspp. of highly similar morphology, which display strict niche separation in terms of host specificity and selectivity. We identify two previously unreported clades within the genusRenicola; however, only one of them is supported by the analysis of adult worms. We also provide comparative measurements of the three examined closely related central European renicolids, and describe the newly proposed tern-specialized speciesRenicola sternaesp. n., which was previously repeatedly misidentified asRenicola paraquinta. Based on the extensive dataset collected in 1962–2015, we update the host spectrum of Renicolidae parasitizing central European birds (Renicola bretensis, R. lari, Renicola mediovitellata, R. pinguis, Renicola secundaandR. sternaesp. n.) and discuss their host-specific prevalence and intensity of infections.


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