Distribution of virulence genes and genotyping of CTX-M-15-producing Klebsiella pneumoniae isolated from patients with community-acquired urinary tract infection (CA-UTI)

2016 ◽  
Vol 100 ◽  
pp. 244-249 ◽  
Author(s):  
Reza Ranjbar ◽  
Hamed Memariani ◽  
Rahim Sorouri ◽  
Mojtaba Memariani
Author(s):  
Alessandra Tammy Hayakawa Ito de Sousa ◽  
Marco Túlio dos Santos Costa ◽  
Herica Makino ◽  
Stéfhano Luis Cândido ◽  
Isabela de Godoy Menezes ◽  
...  

2017 ◽  
Vol 107 ◽  
pp. 44-47 ◽  
Author(s):  
Ali Badamchi ◽  
Hossein Masoumi ◽  
Shima Javadinia ◽  
Ramin Asgarian ◽  
Azardokht Tabatabaee

2020 ◽  
Vol 11 (2) ◽  
pp. 2424-2432
Author(s):  
Nabil Salim Saaid Tuwaij ◽  
Huda Jameel Baker Al-khilkhali ◽  
Haneen Mohamed Mohsen

Klebsiella pneumoniae is a significant concern multidrug-resistant microorganism and a one common gram negative bacteria associated with infections of women urinary tract. Therefore, this work aimed to the molecular screening of Sul(1and 2), Gyr(A and B) and OXA genes among K. pneumoniae isolates in Najaf City, Iraq. Out of 250 urine specimens were collected from women showing symptoms of urinary tract infection during five months January to of May 2019, bacterial growth was157 isolates, included 133 gram negative compared with  24 gram positive bacteria while 98 specimens were no growth. According to the Vitek-2 system, 30 K. pneumoniae isolates were obtained.Data on current work revealed that the 26-35 age group was the highest 14 K. pneumoniae isolates. Results of antimicrobial susceptible recorded all isolates were multi-drug resistant (MDR) and they have a different range of resistance. However, all 30 isolates(100%) resistant to ampicillin drugs, while the lowest rate was 1(3.33%) forImipenemdrug. PCR assay revealed exist of oxa, sul-1, sul-2, gyr-A and gyr-B genes among K. pneumoniae isolates with rates 20(66.66%), 11(36.66%), 22(73.33%), 3(10%) and 17(56.66%) respectively.


Cureus ◽  
2017 ◽  
Author(s):  
Faizan Yasin ◽  
Salman Assad ◽  
Abdul Subhan Talpur ◽  
Mehr Zahid ◽  
Shuja A Malik

Gut Pathogens ◽  
2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Zohreh Ghalavand ◽  
Masoud Alebouyeh ◽  
Kiandokht Ghanati ◽  
Leila Azimi ◽  
Marjan Rashidan

Abstract Background Community-acquired urinary tract infection (CA-UTI) could be caused by endogenous or exogenous routes. To show this relationship, we investigated molecular fingerprints and genotypes of paired Enterococcus faecalis isolated from the urine of symptomatic patients and their fecal samples. Results Out of the studied patients, 63 pairs of E. faecalis isolates were obtained simultaneously from their urine and feces samples. All the strains were sensitive to vancomycin, linezolid, nitrofurantoin, and daptomycin (MIC value: ≤ 4 µg/ml), while resistance to tetracycline (urine: 88.9%; stool: 76.2%) and minocycline (urine: 87.3%, stool: 71.4%) was detected in most of them. The most common detected virulence genes were included efbA, ace, and gelE. RAPD-PCR and PFGE analyses showed the same patterns of molecular fingerprints between paired of the isolates in 26.9% and 15.8% of the patients, respectively. Conclusions Similarity of E. faecalis strains between the urine and feces samples confirmed the occurrence of endogenous infection via contamination with colonized bacteria in the intestinal tract. Carriage of a complete virulence genotype in the responsible strains was statistically in correlation with endogenous UTI, which shows their possible involvement in pathogenicity of uropathogenic E. faecalis strains.


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