Molecular detection of six virulence genes in Pseudomonas aeruginosa isolates detected in children with urinary tract infection

2017 ◽  
Vol 107 ◽  
pp. 44-47 ◽  
Author(s):  
Ali Badamchi ◽  
Hossein Masoumi ◽  
Shima Javadinia ◽  
Ramin Asgarian ◽  
Azardokht Tabatabaee
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Virulence Genes ◽  
Molecular Detection

scholarly journals MOLECULAR DETECTION OF UREC, HPMA, RSBA AND MRPA GENES OF PROTEUS MIRABILIS URINARY TRACT INFECTION IN PATIENT WITH RHEUMATOID ARTHRITIS

2020 ◽  
Vol 51 (Special) ◽  
Author(s):  
AL-Hamdani & AL-Hashimy
Keyword(s):  
Rheumatoid Arthritis ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Proteus Mirabilis ◽  
Blood Test ◽  
Virulence Genes ◽  
Molecular Detection ◽  
Automated System ◽  
Vitek 2

This study was aimed to detect of four important virulence genes (UreC, HpmA, rsbA and mrpA) from Proteus mirabilis bacteria isolated and do the important four blood test (ACPA, RF, ESR, CRP) for patient with urinary tract infection and rheumatoid arthritis in Baghdad/Iraq. Fifty (50) clinical urine samples were collected and cultured on blood agar and macckongy agar. Ten of the samples were identified as P.mirabilis and the automated system VITEK 2 used to complete the identification. Molecular methods using PCR technique targeting UreC, HpmA, rsbA and mrpA genes, using PCR technique and the results were UreC found in 90% of the samples, HpmA 90%, rsbA 100% and mrpA 80%. The P. mirabilis samples were also characterized for antibiotic resistance and the result showed that the majority of samples were show high resistant to Cefotaxime 90%, while the highest susceptibility was against Impenem antibiotic 70%.

Detection of tox A gene in Pseudomonas aeruginosa that isolates from different clinical cases by using PCR.

2018 ◽  
pp. 26
Author(s):  
Rana M. Abdullah Al-Shwaikh ◽  
Abbas Falih Alornaaouti
Keyword(s):  
Molecular Weight ◽  
Pseudomonas Aeruginosa ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Otitis Media ◽  
Urinary Tract Infections ◽  
Gel Electrophoresis ◽  
Wound Infections ◽  
Tract Infections

       Current study obtained (75) isolate of Pseudomonas aeruginosa collected from different cases included : 28 isolates from otitis media, 23 isolates from burn infections, 10 isolates from wound infections, 8 isolates from urinary tract infections and 6 isolates from blood, during the period between 1/9/2014 to 1/11/2014        The result revealed that the tox A gene was present in 54 isolates (72%) of Pseudomonas aeruginosa. The gel electrophoresis showed that the molecular weight of tox A gene was 352 bp. The result shows 17 isolates (60.71%) from otitis media has tox A gene, 18 isolates (78.26%) from burn followed by 8 isolate (80%) from wound infection and 5 isolates (62.5%) from urinary tract infection , finally 6 isolates (100%) from blood have this gene.

scholarly journals Bacteriophage therapy for refractory Pseudomonas aeruginosa urinary tract infection

2011 ◽  
Vol 60 (11) ◽  
pp. 1697-1700 ◽  
Author(s):  
A. Khawaldeh ◽  
S. Morales ◽  
B. Dillon ◽  
Z. Alavidze ◽  
A. N. Ginn ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Bacteriophage Therapy

Therapeutic Efficacy of Sitafloxacin Against Pseudomonas aeruginosa in a Rat Model of Urinary Tract Infection

Drugs ◽  
1999 ◽  
Vol 58 (Supplement 2) ◽  
pp. 412-414 ◽  
Author(s):  
Yuichi Kurosaka ◽  
Yoshihisa Ishida ◽  
Eiko Yamamura ◽  
Tsuyoshi Otani ◽  
Hiromi Kumon
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Rat Model ◽  
Therapeutic Efficacy

Evaluation of Antibacterial Potential of Ethanol Fruit Peel Extract of Mangifera indica against Isolated UTI Pathogens

2021 ◽  
pp. 3998-4005
Author(s):  
Adeyinka A. Aderinola ◽  
Jane Ejiofor ◽  
Lukmon Ogunjimi ◽  
Akanji A. Murtala ◽  
Oladapo E. Oyinloye
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Mangifera Indica ◽  
Inhibition Zone ◽  
Fruit Peel ◽  
Gram Negative ◽  
Peel Extract

Effective use of antimicrobial agents for treatment/management of infectious diseases is decreasing due to emergency of multi-drug and cross resistant strains of pathogenic microbes. Medicinal plants are now increasingly used alone or as an adjunct in the management of infectious diseases as a result of their claimed efficacy and safety. This research focused on evaluating the antimicrobial potential of ethanol fruit peel extract of Mangifera indica against isolated Urinary tract infection (UTI) pathogens. Urinary tract infection (UTI) bacterial strains (staphylococcus aureus, escherichia coli and pseudomonas aeruginosa) were isolated from midstream urine of infected students using standard procedures after which the isolated Urinary tract infection pathogens were subjected to antimicrobial susceptibility test by agar-well diffusion method (Cup plate method) and the mean diameter of growth inhibition zones (n=3) of the extract at different concentrations were compared against the controls (sterile water and ciprofloxacin). Ethanol fruit peel extract of Mangifera indica significantly and dose dependently inhibit the growth of all the isolated Urinary tract infection pathogens with E.coli exhibiting the highest inhibition zone ranging from 16.83 to 28.23 mm, this was followed by pseudomonas aeruginosa with inhibition zone of 24.33 mm and staphylococcus aureus least susceptible with 22.63 diameter of zone inhibition. Ethanol fruit peel extract of Mangifera indica demonstrated a dose dependent antimicrobial activity with more pronounced effect exhibited by E. coli suggesting that the extract is more effective against gram negative bacteria despite their permeability barrier, thus suggesting Mangifera indica fruit peel as a potential candidate for the management of bacterial infections especially those caused by gram negative organisms.

scholarly journals Genetic relatedness of the Enterococcus faecalis isolates in stool and urine samples of patients with community-acquired urinary tract infection

Gut Pathogens ◽  
2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Zohreh Ghalavand ◽  
Masoud Alebouyeh ◽  
Kiandokht Ghanati ◽  
Leila Azimi ◽  
Marjan Rashidan
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Enterococcus Faecalis ◽  
Intestinal Tract ◽  
Virulence Genes ◽  
Genetic Relatedness ◽  
Molecular Fingerprints ◽  
Rapd Pcr ◽  
Mic Value

Abstract Background Community-acquired urinary tract infection (CA-UTI) could be caused by endogenous or exogenous routes. To show this relationship, we investigated molecular fingerprints and genotypes of paired Enterococcus faecalis isolated from the urine of symptomatic patients and their fecal samples. Results Out of the studied patients, 63 pairs of E. faecalis isolates were obtained simultaneously from their urine and feces samples. All the strains were sensitive to vancomycin, linezolid, nitrofurantoin, and daptomycin (MIC value: ≤ 4 µg/ml), while resistance to tetracycline (urine: 88.9%; stool: 76.2%) and minocycline (urine: 87.3%, stool: 71.4%) was detected in most of them. The most common detected virulence genes were included efbA, ace, and gelE. RAPD-PCR and PFGE analyses showed the same patterns of molecular fingerprints between paired of the isolates in 26.9% and 15.8% of the patients, respectively. Conclusions Similarity of E. faecalis strains between the urine and feces samples confirmed the occurrence of endogenous infection via contamination with colonized bacteria in the intestinal tract. Carriage of a complete virulence genotype in the responsible strains was statistically in correlation with endogenous UTI, which shows their possible involvement in pathogenicity of uropathogenic E. faecalis strains.

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