Staphylococcus aureus induces TGF-β1 and bFGF expression through the activation of AP-1 and NF-κB transcription factors in bovine mammary epithelial cells

2018 ◽  
Vol 117 ◽  
pp. 276-284 ◽  
Author(s):  
Jianmei Wu ◽  
Yulin Ding ◽  
Jinling Wang ◽  
Fenglong Wang
Keyword(s):  
Staphylococcus Aureus ◽  
Transcription Factors ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells ◽  
Tgf Β1

scholarly journals Host-pathogen interactions in bovine mammary epithelial cells and HeLa cells by Staphylococcus aureus isolated from subclinical bovine mastitis

2017 ◽  
Vol 100 (8) ◽  
pp. 6414-6421 ◽  
Author(s):  
Ivana G. Castilho ◽  
Stéfani Thais Alves Dantas ◽  
Hélio Langoni ◽  
João P. Araújo ◽  
Ary Fernandes ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Epithelial Cells ◽  
Hela Cells ◽  
Mammary Epithelial Cells ◽  
Bovine Mastitis ◽  
Mammary Epithelial ◽  
Host Pathogen Interactions ◽  
Bovine Mammary Epithelial Cells ◽  
Host Pathogen

scholarly journals Plant Defensin γ-Thionin Induces MAPKs and Activates Histone Deacetylases in Bovine Mammary Epithelial Cells Infected With Staphylococcus aureus

2020 ◽  
Vol 7 ◽  
Author(s):  
Marisol Báez-Magaña ◽  
Nayeli Alva-Murillo ◽  
Ivan Medina-Estrada ◽  
María Teresa Arceo-Martínez ◽  
Joel E. López-Meza ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Epithelial Cells ◽  
Histone Deacetylases ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Plant Defensin ◽  
Bovine Mammary Epithelial Cells

scholarly journals Inhibition of Staphylococcus aureus Invasion into Bovine Mammary Epithelial Cells by Contact with Live Lactobacillus casei

2012 ◽  
Vol 79 (3) ◽  
pp. 877-885 ◽  
Author(s):  
Damien S. Bouchard ◽  
Lucie Rault ◽  
Nadia Berkova ◽  
Yves Le Loir ◽  
Sergine Even
Keyword(s):  
Staphylococcus Aureus ◽  
Epithelial Cells ◽  
Lactobacillus Casei ◽  
Mammary Epithelial Cells ◽  
Control Strategies ◽  
Mammary Epithelial ◽  
Dairy Herds ◽  
Dependent Manner ◽  
Content Type ◽  
Bovine Mammary Epithelial Cells

ABSTRACTStaphylococcus aureusis a major pathogen that is responsible for mastitis in dairy herds.S. aureusmastitis is difficult to treat and prone to recurrence despite antibiotic treatment. The ability ofS. aureusto invade bovine mammary epithelial cells (bMEC) is evoked to explain this chronicity. One sustainable alternative to treat or prevent mastitis is the use of lactic acid bacteria (LAB) as mammary probiotics. In this study, we tested the ability ofLactobacillus caseistrains to prevent invasion of bMEC by twoS. aureusbovine strains, RF122 and Newbould305, which reproducibly induce acute and moderate mastitis, respectively.L. caseistrains affected adhesion and/or internalization ofS. aureusin a strain-dependent manner. Interestingly,L. caseiCIRM-BIA 667 reducedS. aureusNewbould305 and RF122 internalization by 60 to 80%, and this inhibition was confirmed for two otherL. caseistrains, including one isolated from bovine teat canal. The protective effect occurred without affecting bMEC morphology and viability. Once internalized, the fate ofS. aureuswas not affected byL. casei. It should be noted thatL. caseiwas internalized at a low rate but survived in bMEC cells with a better efficiency than that ofS. aureusRF122. Inhibition ofS. aureusadhesion was maintained with heat-killedL. casei, whereas contact between liveL. caseiandS. aureusor bMEC was required to preventS. aureusinternalization. This first study of the antagonism of LAB towardS. aureusin a mammary context opens avenues for the development of novel control strategies against this major pathogen.

scholarly journals Staphylococcus aureus Invasion of Bovine Mammary Epithelial Cells

1996 ◽  
Vol 79 (6) ◽  
pp. 1021-1026 ◽  
Author(s):  
Raul A. Almeida ◽  
Karl R. Matthews ◽  
Eduardo Cifrian ◽  
Albert J. Guidry ◽  
Stephen P. Oliver
Keyword(s):  
Staphylococcus Aureus ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells

scholarly journals Simultaneous lack of catalase and beta-toxin in Staphylococcus aureus leads to increased intracellular survival in macrophages and epithelial cells and to attenuated virulence in murine and ovine models

Microbiology ◽  
2009 ◽  
Vol 155 (5) ◽  
pp. 1505-1515 ◽  
Author(s):  
Susana Martínez-Pulgarín ◽  
Gustavo Domínguez-Bernal ◽  
José A. Orden ◽  
Ricardo de la Fuente
Keyword(s):  
Staphylococcus Aureus ◽  
T Cells ◽  
Epithelial Cells ◽  
Virulence Factors ◽  
Mammary Epithelial Cells ◽  
Intracellular Survival ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells ◽  
Wide Range ◽  
Beta Toxin

Staphylococcus aureus produces a variety of virulence factors that allow it to cause a wide range of infections in humans and animals. In the latter, S. aureus is a leading cause of intramammary infections. The contribution of catalase (KatA), an enzyme implicated in oxidative stress resistance, and beta-toxin (Hlb), a haemolysin, to the pathogenesis of S. aureus is poorly characterized. To investigate the role of these enzymes as potential virulence factors in S. aureus, we examined the intracellular survival of ΔkatA, Δhlb and ΔkatA Δhlb mutants in murine macrophages (J774A.1) and bovine mammary epithelial cells (MAC-T), and their virulence in different murine and ovine models. Catalase was not required for the survival of S. aureus within either J774A.1 or MAC-T cells. However, it was necessary for the intracellular proliferation of the bacterium after invasion of MAC-T cells. In addition, catalase was not needed for the full virulence of S. aureus in mice. Deletion of the hlb gene had no effect on the intracellular survival of S. aureus in J774A.1 cells but did cause a slight increase in survival in MAC-T cells. Furthermore, like catalase, beta-toxin was not required for complete virulence of S. aureus in murine models. Unexpectedly, the ΔkatA Δhlb mutant showed a notably increased persistence in both cell lines, and was significantly less virulent for mice than were the wild-type strain and single mutants. Most interestingly, it was also markedly attenuated in intramammary and subcutaneous infections in ewes and lambs.

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