Mycobacterium tuberculosis Ag85b:hfcγ1 recombinant fusion protein as a selective receptor-dependent delivery system for antigen presentation

2019 ◽  
Vol 129 ◽  
pp. 68-73 ◽  
Author(s):  
Mohsen Karbalaei Zadeh Babaki ◽  
Mahboubeh Taghiabadi ◽  
Saman Soleimanpour ◽  
Masoud Saleh Moghadam ◽  
Arman Mosavat ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Fusion Protein ◽  
Antigen Presentation ◽  
Delivery System ◽  
Recombinant Fusion Protein

scholarly journals Induration or erythema diameter not less than 5 mm as results of recombinant fusion protein ESAT6-CFP10 skin test for detecting M. tuberculosis infection

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Hui Zhang ◽  
Ling Wang ◽  
Feng Li ◽  
Shuihua Lu ◽  
Jielai Xia
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Fusion Protein ◽  
Healthy Volunteers ◽  
Skin Test ◽  
Clinical Diagnosis ◽  
Skin Testing ◽  
Recombinant Fusion Protein ◽  
Phase Iii ◽  
High Consistency ◽  
Diagnostic Indicator

Abstract Background Recombinant fusion protein ESAT6-CFP10 (EC) is a newly developed skin test reagent for detecting Mycobacterium tuberculosis (M. tuberculosis) infection. In this study, we evaluated whether induration and erythema could be used as diagnostic indicators for EC skin test to detect M. tuberculosis infection. Methods A total of 743 tuberculosis patients and 1514 healthy volunteers underwent an EC skin test. The diameters of induration and erythema were measured with Vernier caliper, 24 h, 48 h, and 72 h after skin testing. Related indicators of EC reagent diagnostic test were tested, and the diagnostic effects of the four diagnostic indicators for EC skin test were compared. Results The sensitivity of induration / erythema measurement was lower at 24 h after EC skin test than at 48 h or 72 h (P<0.01). There was no difference in consistency (P = 0.16) between induration with clinical diagnosis, and erythema with clinical diagnosis at 48 h (88.88 and 90.16%, Kappa value was 0.75 and 0.78, respectively). In patients, the sensitivity of erythema measurement was higher than induration measurement (P<0.01). In healthy volunteers, the specificity of erythema measurement was lower than induration at 24 h after skin test, but there was no difference at 48 h after skin test (P = 0.22). In BCG vaccination volunteers, the specificity of induration and erythema were higher than 90%. In addition, there was a high consistency of induration and erythema. When induration or erythema was used as a positive diagnostic indicator, the sensitivity of the EC skin test was improved, and was no different from the other three indicators in terms of specificity and consistency with clinical diagnosis. Conclusions Induration or erythema diameter not less than 5 mm could be used as a diagnostic indicator for detecting M. tuberculosis infection. Trial registration Phase III clinical trial of recombinant Mycobacterium tuberculosis ESAT6-CFP10 allergen; CTR20150695; registered in December 16, 2015.

scholarly journals Structural Characterization of a Recombinant Fusion Protein by Instrumental Analysis and Molecular Modeling

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e57642 ◽  
Author(s):  
Zhigang Wu ◽  
Peng Zhou ◽  
Xiaoxin Li ◽  
Hui Wang ◽  
Delun Luo ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Fusion Protein ◽  
Structural Characterization ◽  
Recombinant Fusion Protein ◽  
Instrumental Analysis

A recombinant fusion protein derived from dog hookworm inhibits autoantibody-induced dermal-epidermal separationex vivo

2015 ◽  
Vol 24 (11) ◽  
pp. 872-878 ◽  
Author(s):  
Annette Kemmer ◽  
Katja Bieber ◽  
Aida Abadpour ◽  
Xinhua Yu ◽  
Nina Mitschker ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Recombinant Fusion Protein

scholarly journals Protective and Therapeutic Efficacy of Mycobacterium smegmatis Expressing HBHA-hIL12 Fusion Protein against Mycobacterium tuberculosis in Mice

PLoS ONE ◽  
2012 ◽  
Vol 7 (2) ◽  
pp. e31908 ◽  
Author(s):  
Shanmin Zhao ◽  
Yong Zhao ◽  
Fengfeng Mao ◽  
Caiqin Zhang ◽  
Bing Bai ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Fusion Protein ◽  
Mycobacterium Smegmatis ◽  
Therapeutic Efficacy
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