Identification and characterization of class 1 integrons among multidrug-resistant uropathogenic Escherichia coli strains in Mexico

2022 ◽  
Vol 162 ◽  
pp. 105348
Author(s):  
Edgar González-Villalobos ◽  
Rosa María Ribas-Aparicio ◽  
Laura Belmont-Monroy ◽  
Gerardo Aparicio-Ozores ◽  
H. Ángel Manjarrez-Hernández ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Uropathogenic Escherichia Coli ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Identification And Characterization

scholarly journals Identification and characterization of two novel ISCR1-associated genes dfrA42 and dfrA43 encoding trimethoprim resistant dihydrofolate reductases

2021 ◽  
Author(s):  
Ling Li ◽  
Mengge Zhang ◽  
Wenjia Wang ◽  
Ruirui Xia ◽  
Yanan Ma ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Complex Class ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Dihydrofolate Reductases ◽  
High Level ◽  
Identification And Characterization

Two novel ISCR1-associated dfr genes, dfrA42 and dfrA43, were identified from trimethoprim (TMP)-resistant Proteus strains and were shown to confer high level TMP resistance (MIC ≥ 1024 mg/L) when cloned into Escherichia coli. These genes were hosted by complex class 1 integrons suggesting their potentials for dissemination. Analysis of enzymatic parameters and TMP affinity were performed, suggesting that the mechanism of TMP resistance for these novel DHFRs is the reduction of binding with TMP.

scholarly journals Occurrence and characterization of class 1 integrons in Escherichia coli from healthy individuals and those with urinary infection

2017 ◽  
Vol 66 (5) ◽  
pp. 577-583 ◽  
Author(s):  
Clarisse Oliveira-Pinto ◽  
Cristiane Diamantino ◽  
Patrícia L Oliveira ◽  
Mariana P Reis ◽  
Patrícia S Costa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Infection ◽  
Healthy Individuals ◽  
Class 1 Integrons ◽  
Class 1

scholarly journals Characterization of Multidrug-Resistant Escherichia coli Isolates from Animals Presenting at a University Veterinary Hospital

2011 ◽  
Vol 77 (20) ◽  
pp. 7104-7112 ◽  
Author(s):  
Maria Karczmarczyk ◽  
Yvonne Abbott ◽  
Ciara Walsh ◽  
Nola Leonard ◽  
Séamus Fanning
Keyword(s):  
Escherichia Coli ◽  
Molecular Mechanisms ◽  
Gene Array ◽  
Multidrug Resistant ◽  
Genetic Determinants ◽  
Gene Encoding ◽  
Content Type ◽  
E Coli ◽  
Class 1

ABSTRACTIn this study, we examined molecular mechanisms associated with multidrug resistance (MDR) in a collection ofEscherichia coliisolates recovered from hospitalized animals in Ireland. PCR and DNA sequencing were used to identify genes associated with resistance. Class 1 integrons were prevalent (94.6%) and contained gene cassettes recognized previously and implicated mainly in resistance to aminoglycosides, β-lactams, and trimethoprim (aadA1,dfrA1-aadA1,dfrA17-aadA5,dfrA12-orfF-aadA2,blaOXA-30-aadA1,aacC1-orf1-orf2-aadA1,dfr7). Class 2 integrons (13.5%) contained thedfrA1-sat1-aadA1gene array. The most frequently occurring phenotypes included resistance to ampicillin (97.3%), chloramphenicol (75.4%), florfenicol (40.5%), gentamicin (54%), neomycin (43.2%), streptomycin (97.3%), sulfonamide (98.6%), and tetracycline (100%). The associated resistance determinants detected includedblaTEM,cat,floR,aadB,aphA1,strA-strB,sul2, andtet(B), respectively. TheblaCTX-M-2gene, encoding an extended-spectrum β-lactamase (ESβL), andblaCMY-2, encoding an AmpC-like enzyme, were identified in 8 and 18 isolates, respectively. The mobility of the resistance genes was demonstrated using conjugation assays with a representative selection of isolates. High-molecular-weight plasmids were found to be responsible for resistance to multiple antimicrobial compounds. The study demonstrated that animal-associated commensalE. coliisolates possess a diverse repertoire of transferable genetic determinants. Emergence of ESβLs and AmpC-like enzymes is particularly significant. To our knowledge, theblaCTX-M-2gene has not previously been reported in Ireland.

Diversity of Gene Cassette Promoter Variants of Class 1 Integrons in Uropathogenic Escherichia coli

2013 ◽  
Vol 67 (5) ◽  
pp. 543-549 ◽  
Author(s):  
Quhao Wei ◽  
Xiaofei Jiang ◽  
Min Li ◽  
Gang Li ◽  
Qingfeng Hu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Cassette ◽  
Uropathogenic Escherichia Coli ◽  
Class 1 Integrons ◽  
Class 1

scholarly journals Identification and Characterization of Class 1 Integron Resistance Gene Cassettes among Salmonella Strains Isolated from Imported Seafood

2008 ◽  
Vol 75 (4) ◽  
pp. 1192-1196 ◽  
Author(s):  
Ashraf A. Khan ◽  
Elizabeth Ponce ◽  
M. S. Nawaz ◽  
Chorng-Ming Cheng ◽  
Junaid A. Khan ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Southern Hybridization ◽  
Multidrug Resistant ◽  
The Philippines ◽  
Streptomycin Resistance ◽  
Class 1 Integron ◽  
Class 1 ◽  
Identification And Characterization ◽  
Multiple Antibiotics

ABSTRACT A total of 210 Salmonella isolates, representing 64 different serovars, were isolated from imported seafood samples, and 55/210 isolates were found to be resistant to at least one antibiotic. Class 1 integrons from three multidrug-resistant Salmonella enterica strains (Salmonella enterica serovars Newport [strain 62], Typhimurium var. Copenhagen [strain 629], and Lansing [strain 803], originating from Hong Kong, the Philippines, and Taiwan, respectively) were characterized. Southern hybridization of plasmids isolated from these strains, using a class 1 integron probe, showed that trimethoprim-sulfamethoxazole and streptomycin resistance genes were located on a megaplasmid in strain 629. Our study indicates that imported seafood could be a reservoir for Salmonella isolates resistant to multiple antibiotics.

Characterization of Antibiotic Resistance in Escherichia coli Isolated from Shrimps and Their Environment

2014 ◽  
Vol 77 (8) ◽  
pp. 1394-1401 ◽  
Author(s):  
KANJANA CHANGKAEW ◽  
FUANGFA UTRARACHKIJ ◽  
KANOKRAT SIRIPANICHGON ◽  
CHIE NAKAJIMA ◽  
ORASA SUTHIENKUL ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Multidrug Resistant ◽  
Drug Resistant ◽  
E Coli ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Resistance Rates ◽  
Drug Resistant Strains ◽  
Shrimp Farms

Antimicrobial resistance in bacteria associated with food and water is a global concern. To survey the risk, 312 Escherichia coli isolates from shrimp farms and markets in Thailand were examined for susceptibility to 10 antimicrobials. The results showed that 17.6% of isolates (55 of 312) were resistant to at least one of the tested drugs, and high resistance rates were observed to tetracycline (14.4%; 45 of 312), ampicillin (8.0%; 25 of 312), and trimethroprim (6.7%; 21 of 312); 29.1% (16 of 55) were multidrug resistant. PCR assay of the tet(A), tet(B), tet(C), tet(D), tet(E), and tet(G) genes detected one or more of these genes in 47 of the 55 resistant isolates. Among these genes, tet(A) (69.1%; 38 of 55) was the most common followed by tet(B) (56.4%; 31 of 55) and tet(C) (3.6%; 2 of 55). The resistant isolates were further investigated for class 1 integrons. Of the 55 resistant isolates, 16 carried class 1 integrons and 7 carried gene cassettes encoding trimethoprim resistance (dfrA12 or dfrA17) and aminoglycosides resistance (aadA2 or aadA5). Two class 1 integrons, In54 (dfrA17-aadA5) and In27 (dfrA12-orfF-aadA2), were found in four and three isolates, respectively. These results indicate a risk of drug-resistant E. coli contamination in shrimp farms and selling places. The occurrence of multidrug-resistant E. coli carrying tet genes and class 1 integrons indicates an urgent need to monitor the emergence of drug-resistant E. coli to control the dissemination of drug-resistant strains and the further spread of resistance genes to other pathogenic bacteria.

Sign in / Sign up

Export Citation Format

Share Document