scholarly journals Pseudomonas protegens MP12: A plant growth-promoting endophytic bacterium with broad-spectrum antifungal activity against grapevine phytopathogens

2019 ◽  
Vol 219 ◽  
pp. 123-131 ◽  
Author(s):  
Marco Andreolli ◽  
Giacomo Zapparoli ◽  
Elisa Angelini ◽  
Gianluca Lucchetta ◽  
Silvia Lampis ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Plant Growth ◽  
Broad Spectrum ◽  
Endophytic Bacterium ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sujit Shah ◽  
Krishna Chand ◽  
Bhagwan Rekadwad ◽  
Yogesh S. Shouche ◽  
Jyotsna Sharma ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Colonization ◽  
Endophytic Bacterium ◽  
Epifluorescence Microscopy ◽  
In Vitro Cultivation ◽  
Rrna Gene ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment.

scholarly journals Genome Insights into the Novel Species Jejubacter calystegiae, a Plant Growth-Promoting Bacterium in Saline Conditions

Diversity ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 24
Author(s):  
Ling Min Jiang ◽  
Yong Jae Lee ◽  
Ho Le Han ◽  
Myoung Hui Lee ◽  
Jae Cheol Jeong ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Novel Species ◽  
Endophytic Bacterium ◽  
Morning Glory ◽  
Plant Growth Promoting Bacteria ◽  
Illumina Hiseq ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Sequencing Platforms

Jejubacter calystegiae KSNA2T, a moderately halophilic, endophytic bacterium isolated from beach morning glory (Calystegia soldanella), was determined to be a novel species in a new genus in the family Enterobacteriaceae. To gain insights into the genetic basis of the salinity stress response of strain KSNA2T, we sequenced its genome using two complementary sequencing platforms (Illumina HiSeq and PacBio RSII). The genome contains a repertoire of metabolic pathways, such as those for nitrogen, phosphorus, and some amino acid metabolism pathways. Functional annotation of the KSNA2T genome revealed several genes involved in salt tolerance pathways, such as those encoding sodium transporters, potassium transporters, and osmoprotectant enzymes. Plant growth-promoting bacteria-based experiments indicated that strain KSNA2T promotes the germination of vegetable seeds in saline conditions. Overall, the genetic and biological analyses of strain KSNA2T provide valuable insights into bacteria-mediated salt tolerance in agriculture.

scholarly journals Draft Genome Sequence of Brevibacillus brevis DZQ7, a Plant Growth-Promoting Rhizobacterium with Broad-Spectrum Antimicrobial Activity

2015 ◽  
Vol 3 (4) ◽  
Author(s):  
Qihui Hou ◽  
Chengqiang Wang ◽  
Xiaoyang Hou ◽  
Zhilin Xia ◽  
Jiangping Ye ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Plant Growth ◽  
Genome Sequence ◽  
Broad Spectrum ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Functional Genes ◽  
Plant Growth Promoting ◽  
Brevibacillus Brevis ◽  
Growth Promoting

Brevibacillus brevis DZQ7 is a plant growth-promoting rhizobacterium (PGPR) isolated from tobacco rhizosphere. Here, we report the draft genome sequence of B. brevis DZQ7. Several functional genes related to antimicrobial activity were identified in the genome.

scholarly journals Mechanism of phosphate solubilization and antifungal activity of Streptomyces spp. isolated from wheat roots and rhizosphere and their application in improving plant growth

Microbiology ◽  
2014 ◽  
Vol 160 (4) ◽  
pp. 778-788 ◽  
Author(s):  
Rahul Jog ◽  
Maharshi Pandya ◽  
G. Nareshkumar ◽  
Shalini Rajkumar
Keyword(s):  
Gene Expression ◽  
Antifungal Activity ◽  
Plant Growth ◽  
Potential Field ◽  
Phosphate Solubilization ◽  
Isocitrate Lyase ◽  
Malate Synthase ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting

The application of plant-growth-promoting rhizobacteria (PGPR) at field scale has been hindered by an inadequate understanding of the mechanisms that enhance plant growth, rhizosphere incompetence and the inability of bacterial strains to thrive in different soil types and environmental conditions. Actinobacteria with their sporulation, nutrient cycling, root colonization, bio-control and other plant-growth-promoting activities could be potential field bio-inoculants. We report the isolation of five rhizospheric and two root endophytic actinobacteria from Triticum aestivum (wheat) plants. The cultures exhibited plant-growth-promoting activities, namely phosphate solubilization (1916 mg l−1), phytase (0.68 U ml−1), chitinase (6.2 U ml−1), indole-3-acetic acid (136.5 mg l−1) and siderophore (47.4 mg l−1) production, as well as utilizing all the rhizospheric sugars under test. Malate (50–55 mmol l−1) was estimated in the culture supernatant of the highest phosphate solublizer, Streptomyces mhcr0816. The mechanism of malate overproduction was studied by gene expression and assays of key glyoxalate cycle enzymes – isocitrate dehydrogenase (IDH), isocitrate lyase (ICL) and malate synthase (MS). The significant increase in gene expression (ICL fourfold, MS sixfold) and enzyme activity (ICL fourfold, MS tenfold) of ICL and MS during stationary phase resulted in malate production as indicated by lowered pH (2.9) and HPLC analysis (retention time 13.1 min). Similarly, the secondary metabolites for chitinase-independent biocontrol activity of Streptomyces mhcr0817, as identified by GC-MS and 1H-NMR spectra, were isoforms of pyrrole derivatives. The inoculation of actinobacterial isolate mhce0811 in T. aestivum (wheat) significantly improved plant growth, biomass (33 %) and mineral (Fe, Mn, P) content in non-axenic conditions. Thus the actinobacterial isolates reported here were efficient PGPR possessing significant antifungal activity and may have potential field applications.

scholarly journals Draft Genome Sequence of the Plant Growth-Promoting Cupriavidus gilardii Strain JZ4 Isolated from the Desert Plant Tribulus terrestris

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Feras F. Lafi ◽  
Ameerah Bokhari ◽  
Intikhab Alam ◽  
Vladimir B. Bajic ◽  
Heribert Hirt ◽  
...  
Keyword(s):  
Plant Growth ◽  
Genome Sequence ◽  
Draft Genome ◽  
Endophytic Bacterium ◽  
Draft Genome Sequence ◽  
Desert Plant ◽  
Tribulus Terrestris ◽  
Plant Growth Promoting ◽  
Plant Growth Promoting Activity ◽  
Growth Promoting

We isolated the plant endophytic bacterium Cupriavidus gilardii strain JZ4 from the roots of the desert plant Tribulus terrestris , collected from the Jizan region, Saudi Arabia. We report here the draft genome sequence of JZ4, together with several enzymes related to plant growth-promoting activity, environmental adaption, and antifungal activity.

scholarly journals Plant growth-promoting and antifungal activity of yeasts from dark chestnut soil

2015 ◽  
Vol 175 ◽  
pp. 78-83 ◽  
Author(s):  
Lyudmila V. Ignatova ◽  
Yelena V. Brazhnikova ◽  
Ramza Z. Berzhanova ◽  
Togzhan D. Mukasheva
Keyword(s):  
Antifungal Activity ◽  
Plant Growth ◽  
Chestnut Soil ◽  
Dark Chestnut Soil ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Phenotypic and genotypic characterization of inhabitant PGPR strains of Pseudomonas from apple orchards

2015 ◽  
Vol 7 (2) ◽  
pp. 893-902
Author(s):  
Kumari Manorma ◽  
Shweta Sharma ◽  
Mohinder Kaur
Keyword(s):  
Antifungal Activity ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Siderophore Production ◽  
Pseudomonas Sp ◽  
Genotypic Analysis ◽  
Plant Growth Promoting ◽  
Growth Promoting

Aim of present research was to isolate and characterize the Pseudomonas strains phenotypically and genotypically from the rhizospheric soil of apple orchard at Maggota (Shimla district) Himachal Pradesh. Phenotypic identification of the test isolates was based on morphological, physiological and biochemical characterization of the bacteria followed by genotypic analysis using rRNA gene sequencing and RAPD-PCR analysis. The fourteen Pseudomonas sp. isolates were screened out for various plant growth promoting activities such as siderophore production, antifungal activity, phosphate solubilisation, HCN and ammonia production, production of plant growth regulators and lytic enzymes. Isolates showed production of plant growth regulators (auxins, gibberellins and cytokinins) in the range of 19.67-83.33μg/ml, 21.00-58.67 μg/ml and 12.33-43.33 μg/ml respectively. Pseudomonas strains showed phosphate solubilising activity in the range of 12.33-63.33 Pi μg/ml, 53.66-93.44 % SU siderophore production and 11.33-96.33mm (diameter) protease activity in plate assay. Five Pseudomonas isolates i.e. An-16-kul, An-1-mag, An-2-mag, An-3-mag and An-6-mag showed maximum antifungal activity against plant pathogenic fungi. Therefore, the aim of present investigation was to study multifarious plant growth promoting qualities of Pseudomonas sp. and to select more efficient PGPR strain of fluorescent Pseudomonas sp. which can be further used as biofertilizer.

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