A reversed-phase air-assisted dispersive liquid-liquid microextraction coupled with colorimetric paper-based analytical device for the determination of glycerol, calcium and magnesium in biodiesel samples

2019 ◽  
Vol 150 ◽  
pp. 104134 ◽  
Author(s):  
Andrey Shishov ◽  
Ivan Trufanov ◽  
Daria Nechaeva ◽  
Andrey Bulatov
Keyword(s):  
Reversed Phase ◽  
Calcium And Magnesium ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction ◽  
Analytical Device

scholarly journals Determination of Ketosteroids in Human Urine Using Dispersive Liquid-Liquid Microextraction and Ultra High-Performance Liquid Chromatography-High Resolution Mass Spectrometry

2021 ◽  
Vol 76 (11) ◽  
pp. 1305-1311
Author(s):  
E. V. Dmitrieva ◽  
A. Z. Temerdashev ◽  
A. K. Osipova
Keyword(s):  
Mass Spectrometry ◽  
Human Urine ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
High Sensitivity ◽  
Reversed Phase ◽  
Flight Mass Spectrometry ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

Abstract A method is proposed for the determination of some ketosteroids in human urine, including enzymatic hydrolysis using β-glucuronidase from E. coli followed by dispersive liquid-liquid microextraction, derivatization of analytes with hydroxylamine, and detection by reversed-phase ultra-HPLC–quadrupole time-of-flight mass spectrometry. Optimization of extraction and derivatization conditions of the studied compounds made it possible to find that the highest recoveries were achieved using an acetone–chloroform mixture as a dispersant and an extractant, and the completeness of the derivatization reaction was achieved by thermostating the sample at 70°C for 90 min. The proposed method has high sensitivity (limits of detection in the range of 0.1–0.25 ng/mL) and a wide linearity range.

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