Effects of atrazine on female Wistar rats: Morphological alterations in ovarian follicles and immunocytochemical labeling of 90kDa heat shock protein

The purpose of this study was to investigate the influence of heat treatment on glucocorticoid (GC)-induced myopathy. Eight-week-old Wistar rats were randomly assigned to the control, Dex, and Dex + Heat groups. Dexamethasone (2 mg/kg) was injected subcutaneously 6 days per week for 2 weeks in the Dex and Dex + Heat group. In the Dex + Heat group, heat treatment was performed by immersing hindlimbs in water at 42 °C for 60 min, once every 3 days for 2 weeks. The extensor digitorum longus muscle was extracted following 2 weeks of experimentation. In the Dex + Heat group, muscle fiber diameter, capillary/muscle fiber ratio, and level of heat shock protein 72 were significantly higher and atrogene expression levels were significantly lower than in the Dex group. Our results suggest that heat treatment inhibits the development of GC-induced myopathy by decreasing atrogene expression and increasing angiogenesis.

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Gossypol Promotes Degeneration of Ovarian Follicles in Rats

The Scientific World JOURNAL ◽

10.1155/2014/986184 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 4

Author(s):

Ivana Cristina Nunes Gadelha ◽

Michelly Fernandes de Macedo ◽

Sílvia Catarina Salgado Oloris ◽

Marilia Martins Melo ◽

Benito Soto-Blanco

Keyword(s):

Estrous Cycle ◽

Wistar Rats ◽

Serum Levels ◽

Ovarian Follicles ◽

The Other ◽

Control Group ◽

Stages Of Development ◽

Hormone Levels ◽

Stage Of Development ◽

Female Wistar Rats

The present study aimed to determine if gossypol interferes with ovarian follicles in rats. Twenty-four female Wistar rats were assigned to two equal groups: one control group and the other dosed with gossypol (25 mg/kg/day, subcutaneously) for 15 days. Ovarian follicles were histologically classified according to the stage of development and as normal or atretic. Gossypol treatment reduced the length of estrous with an increase in the duration of the diestrus phase. This compound was responsible for reduced serum levels of T4 and progesterone. Treatment with gossypol was responsible for a significant reduction in the number of normal ovarian follicles and a significant increase in the number of atretic follicles, both in all stages of development. Thus, treatment of rats with gossypol was responsible for reduction in the number of viable follicles and changes in hormone levels that resulted in interference of the estrous cycle.

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ANTIFERTILITY ACTIVITY AND CONTRACEPTIVE POTENTIAL OF THE HYDROALCOHOLIC RHIZOME EXTRACT OF TRILLIUM GOVANIANUM IN FEMALE WISTAR RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i11.27420 ◽

2018 ◽

Vol 11 (11) ◽

pp. 329

Author(s):

Shivam Sharma ◽

Vineet Mehta ◽

Parul Sharma ◽

Kritika Jaggi ◽

Malairaman Udayabanu ◽

...

Keyword(s):

Wistar Rats ◽

Herbal Remedy ◽

Elevated Serum ◽

Morphological Alterations ◽

Estrogenic Potential ◽

Endometrial Thickening ◽

Female Wistar Rats ◽

Implantation Sites ◽

Trillium Govanianum ◽

Dose Dependent

Objective: Trillium govanianum is used in several traditional containing steroids and sex hormones for the management of inflammation, menstrual disorders, sex-related disorders, and antiseptic. The present study was aimed to investigate the antifertility potential of hydroalcoholic rhizome extract of T. govanianum and to explore the possible mechanism of action. Methods: Anti-implantation activity of T. govanianum rhizome extract (125 and 250 mg/kg; p.o.) was performed in female Wistar rats with proven fertility, and its estrogenic/antiestrogenic effect was evaluated in ovariectomized females. 17-α-ethinylestradiol (1 μg/rat/day; s.c.) or plant extract was administered for 11 days after which animals were sacrificed. Percentage inhibition of implantation sites, serum estrogen levels, changes in body and uterus weight, and morphological alterations in the uterus and ovaries were evaluated. Results: T. govanianum treatment resulted in increased uterus weight and induced dose-dependent anti-implantation effect, with 100% implantation inhibition at 250 mg/kg dose. Anti-implantation effects of T. govanianum were associated with endometrial thickening and significantly elevated serum estrogen levels. Moreover, estrogenic/antiestrogenic studies revealed that T. govanianum possessed strong estrogenic effect; however, the effect was saturable. Conclusion: T. govanianum possesses antifertility activity which can be attributed to its strong estrogenic potential and uterine thickening. Moreover, it could find a clinical application as a safer and efficacious birth control herbal remedy.

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High Mobility Group Box 1 and Heat Shock Protein-70 Expression Post (-)-Epigallocatechin-3-Gallate in East Java Green Tea Methanolic Extract Administration During Orthodontic Tooth Movement in Wistar Rats

Pesquisa Brasileira em Odontopediatria e Clínica Integrada ◽

10.1590/pboci.2020.040 ◽

2020 ◽

Vol 20 ◽

Cited By ~ 1

Author(s):

Alexander Patera Nugraha ◽

Ida Bagus Narmada ◽

Putri Intan Sitasari ◽

Fidiyah Inayati ◽

Rahendra Wira ◽

...

Keyword(s):

Heat Shock ◽

Heat Shock Protein ◽

Green Tea ◽

Heat Shock Protein 70 ◽

Wistar Rats ◽

Tooth Movement ◽

Methanolic Extract ◽

Orthodontic Tooth Movement ◽

High Mobility ◽

High Mobility Group

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Study of effects of thermal preconditioning and quercetin on seizure activity and content of heat shock protein 70 kDa in blood plasma in Wistar rats

PsycEXTRA Dataset ◽

10.1037/e516062012-022 ◽

2007 ◽

Author(s):

I. V. Ekimova ◽

L. E. Nitsinskaya ◽

IuF Pastukhov ◽

I. V. Guzhova

Keyword(s):

Heat Shock ◽

Heat Shock Protein ◽

Blood Plasma ◽

Heat Shock Protein 70 ◽

Wistar Rats ◽

Seizure Activity ◽

Thermal Preconditioning ◽

In Blood Plasma

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Heat Shock Proteins (Hsp): Classifications and Its Involvement in Health and Disease

Research in Pharmacy and Health Sciences ◽

10.32463/rphs.2017.v03i03.15 ◽

2017 ◽

Vol 3 (3) ◽

pp. 334-337

Author(s):

Karuna Datta ◽

Kshitij Rahalkar ◽

Dinesh Kumar Dubey

Keyword(s):

Wound Healing ◽

Heat Shock ◽

Heat Shock Proteins ◽

Heat Shock Protein ◽

High Pressures ◽

Cellular Proteins ◽

Morphological Alterations ◽

Health And Disease ◽

Folded Proteins ◽

Shock Proteins

For the reason that no model will ever totally replicate clinical human wound healing, it is necessary that the model operated be selected with care. Heat shock proteins (HSP) are articulated in response to numerous biological stresses, comprising heat, high pressures, and toxic composites. It is also one of the mainly bountiful cellular proteins found under non-stress situation.Hsp70 and Hsp90 refer to families of heat shock proteins on the order of 70,90 kilodaltons in size, respectively. The small 8-kilodalton protein ubiquitin, which marks proteins for degradation, also has features of a heat shock protein. Cells are attentive about getting these folds right for the reason that mis folded proteins can change the normal life of the cell. In some cases change is good, in others deadly. When Heat Shock Proteins90 is conceded the number of morphological alterations upsurges, which lead to creation of inactive or abnormally active polypeptides.

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Effects of alcoholic extract of Myristica fragrans (nutmeg) on pregnancy outcome in female Wistar rats

Journal of Applied Sciences and Environmental Management ◽

10.4314/jasem.v25i8.31 ◽

2021 ◽

Vol 25 (8) ◽

pp. 1483-1491

Author(s):

C.L. Sakpa ◽

D.O. Eguavoen

Keyword(s):

Wistar Rats ◽

Ovarian Follicles ◽

Control Group ◽

Alcoholic Extract ◽

Alcohol Extract ◽

Orogastric Tube ◽

Group 4 ◽

Myristica Fragrans ◽

Female Wistar Rats ◽

Group 2

Myristica fragrans has been reported to be a medicinal plant as it possesses hepatoprotective, antimicrobial, anti-oxidant, antiulcer, anticancer and immunostimulatory properties, as well as antifertility potentials. This study therefore aimed to investigate the effects of alcoholic extract of M. fragrans on dam size, litter size, pup weight, ovarian follicles, endometrial glands and histopathological indices on pregnancy outcomes in female Wistar rats using standard methods. Pregnancy was induced in forty-eight (48) female Wistar rats that were randomly selected and assigned into four (4) groups of twelve (12) rats each: Group 1 (pregestational phase of pregnancy), Group 2 (germinal phase of pregnancy), Group 3 (embryonic phase of pregnancy) and Group 4 (Fetal phase of pregnancy). The treated animals all received 1500 mg of alcoholic extract of M. fragrans. Administration of distilled water and the extract was by orogastric tube. Data were subjected to statistical analysis using the IBM SPSS statistics software (Statistical Package for Social Science) (Version 25) and relevant statistical values were obtained. The extract prevented the proliferation of ovarian follicles and endometrial glands. In addition, during the pregestational, germinal and embryonic phases, there were significant increases in weight of pups when the treated animals were compared to the control group. The dams also showed decreases in body weight during the embryonic phase. This study therefore demonstrated that alcohol extract of Myristica fragrans may serve as a contraceptive agent in view of its anti-fertility effect.

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