Biosynthesis of silver nanoparticles using chitosan immobilized Bacillus cereus: Nanocatalytic studies

Amongst serious biotic factors deteriorating crop yield, the most destructive pathogen of rice is Xanthomonas oryzae pv. oryzae (Xoo), which causes bacterial leaf blight (BLB) disease. This study involved targeted use of biogenic silver nanoparticles (AgNPs) to control BLB in order to cope with the disadvantages of chemical disease control. AgNPs were biologically synthesized from natively isolated Bacillus cereus strain SZT1, which was identified through 16S rRNA gene sequence analysis. Synthesis of AgNPs in bacterial culture supernatant was confirmed through UV-VIS spectroscopy. Fourier transform infrared spectroscopy (FTIR) confirmed that the existence of AgNPs was stabilized with proteins and alcoholic groups. X-ray diffraction (XRD) data revealed the crystalline nature and imaging with scanning electron microscopy (SEM) and transmission electron microscopy (TEM), showing the spherical shape of AgNPs with particle sizes ranging from 18 to 39 nm. The silver presence in AgNPs was further confirmed by energy dispersive spectra. Biogenic AgNPs showed substantial antibacterial activity (24.21 ± 1.01 mm) for Xoo. In a pot experiment, AgNPs were found to be effective weapons for BLB by significantly increasing the plant biomass with a decreased cellular concentration of reactive oxygen species and increased concentration of antioxidant enzyme activity.

Download Full-text

Silver nanoparticles from Prosopis glandulosa and their potential application as biocontrol of Acinetobacter calcoaceticus and Bacillus cereus

Chemical Speciation and Bioavailability ◽

10.1080/09542299.2016.1252693 ◽

2016 ◽

Vol 29 (1) ◽

pp. 1-5 ◽

Cited By ~ 9

Author(s):

Ali Abdelmoteleb ◽

Benjamín Valdez-Salas ◽

Carlos Ceceña-Duran ◽

Olivia Tzintzun-Camacho ◽

Federico Gutiérrez-Miceli ◽

...

Keyword(s):

Silver Nanoparticles ◽

Bacillus Cereus ◽

Potential Application ◽

Acinetobacter Calcoaceticus ◽

Prosopis Glandulosa

Download Full-text

Effect of silver nanoparticles and Bacillus cereus LPR2 on the growth of Zea mays

Scientific Reports ◽

10.1038/s41598-020-77460-w ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Pankaj Kumar ◽

Vikas Pahal ◽

Arti Gupta ◽

Ruchi Vadhan ◽

Harish Chandra ◽

...

Keyword(s):

Silver Nanoparticles ◽

Zea Mays ◽

Plant Growth ◽

Bacillus Cereus ◽

Plant Growth Promoting Rhizobacteria ◽

Maximum Growth ◽

Macrophomina Phaseolina ◽

Rrna Gene ◽

Vis Spectroscopy

AbstractThe effect of Plant Growth Promoting Rhizobacteria (Bacillus sp.) and silver nanoparticles on Zea mays was evaluated. The silver nanoparticles were synthesized from Tagetes erecta (Marigold) leaf and flower extracts, whereas PGPR isolated from spinach rhizosphere. The silver nanoparticles (AgNPs) were purified using ultra centrifugation and were characterized using UV–Vis spectroscopy at gradient wavelength and also by High Resolution Transmission Electron microscopy (HRTEM). The average particles size of AgNPs was recorded approximately 60 nm. Almost all potential isolates were able to produce Indole Acetic Acid (IAA), ammonia and Hydrogen cyanide (HCN), solubilized tricalcium phosphate and inhibited the growth of Macrophomina phaseolina in vitro but the isolate LPR2 was found the best among all. On the basis of 16S rRNA gene sequence, the isolate LPR2 was characterized as Bacillus cereus LPR2. The maize seeds bacterized with LPR2 and AgNPs individually showed a significant increase in germination (87.5%) followed by LPR2 + AgNPs (75%). But the maximum growth of root and shoot of maize plant was observed in seeds coated with LPR2 followed by AgNPs and a combination of both. Bacillus cereus LPR2 and silver nanoparticles enhanced the plant growth and LPR2 strongly inhibited the growth of deleterious fungal pathogen. Therefore, LPR2 and AgNPs could be utilized as bioinoculant and growth stimulator, respectively for maize.

Download Full-text

Silver Nanoparticles Synthesized by Using the Endophytic Bacterium Pantoea ananatis are Promising Antimicrobial Agents against Multidrug Resistant Bacteria

Molecules ◽

10.3390/molecules23123220 ◽

2018 ◽

Vol 23 (12) ◽

pp. 3220 ◽

Cited By ~ 20

Author(s):

Tahmina Monowar ◽

Md. Rahman ◽

Subhash Bhore ◽

Gunasunderi Raju ◽

Kathiresan Sathasivam

Keyword(s):

Electron Microscopy ◽

Escherichia Coli ◽

Staphylococcus Aureus ◽

Silver Nanoparticles ◽

Candida Albicans ◽

Bacillus Cereus ◽

Antimicrobial Agents ◽

Endophytic Bacterium ◽

Multidrug Resistant ◽

Pantoea Ananatis

Antibiotic resistance is one of the most important global problems currently confronting the world. Different biomedical applications of silver nanoparticles (AgNPs) have indicated them to be promising antimicrobial agents. In the present study, extracellular extract of an endophytic bacterium, Pantoea ananatis, was used for synthesis of AgNPs. The synthesized AgNPs were characterized by UV–Vis spectroscopy, FTIR, transmission electron microscopy (TEM), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX), and Zeta potential. The antimicrobial potential of the AgNPs against pathogenic Staphylococcus aureus subsp. aureus (ATCC 11632), Bacillus cereus (ATCC 10876), Escherichia coli (ATCC 10536), Pseudomonas aeruginosa (ATCC 10145) and Candida albicans (ATCC 10231), and multidrug resistant (MDR) Streptococcus pneumoniae (ATCC 700677), Enterococcus faecium (ATCC 700221) Staphylococcus aureus (ATCC 33592) Escherichia coli (NCTC 13351) was investigated. The synthesized spherical-shaped AgNPs with a size range of 8.06 nm to 91.32 nm exhibited significant antimicrobial activity at 6 μg/disc concentration against Bacillus cereus (ATCC 10876) and Candida albicans (ATCC 10231) which were found to be resistant to conventional antibiotics. The synthesized AgNPs showed promising antibacterial efficiency at 10 µg/disc concentration against the MDR strains. The present study suggests that AgNPs synthesized by using the endophytic bacterium P. ananatis are promising antimicrobial agent.

Download Full-text

Enhancement of antibacterial properties of various polymers functionalized with silver nanoparticles

Biointerface Research in Applied Chemistry ◽

10.33263/briac0103.592598 ◽

2019 ◽

Vol 10 (3) ◽

pp. 5592-5598

Keyword(s):

Escherichia Coli ◽

Silver Nanoparticles ◽

Surface Morphology ◽

Bacillus Cereus ◽

Water Absorption ◽

Antibacterial Properties ◽

Antibacterial Activities ◽

Color Transformation ◽

Green Procedure

A new green deposition of silver nanoparticles (AgNPs) on polymers was proposed in this work. In-situ synthesis of AgNPs on polymers was achieved via a green procedure using natural reducing agents, which are Ageratum conyzoidez and Mikania micrantha. Several characterizations of the treated polymers such as color transformation, surface morphology, elemental contents, and water absorption were comprehensively evaluated. For the application, the treated polymers were then tested against waterborne bacteria, which are Escherichia coli and Bacillus cereus. Rapid deposition of AgNPs via the presently biological method can be successfully achieved as observed via surface morphology analysis and energy dispersive X-ray investigation. Water absorption capabilities of the polymers can be decreased after attaching with AgNPs, which can also probably contribute to the enhancement of their antibacterial activities. This study observed that the treated polymers showed excellent antibacterial activities against Escherichia coli and Bacillus cereus. The findings of this study are useful in designing water purifiers to disinfect contaminated water.

Download Full-text