Biomonitoring of DNA damage in peripheral blood lymphocytes of subjects with dental restorative fillings

Abstract Background: Smoking has been shown to have a positive effect on DNA damage in almost all the cells of the body. Quantitative analysis of this damage will help in assessing the etiopathogenesis of various nicotine induced damage to the body. Comet assay has been an emerging tool in this regard and hence was applied by us to estimate the severity of DNA damage in smokers. Aims & Objectives: To evaluate the DNA genotoxicity in peripheral blood lymphocytes in smokers and their comparison with non smokers & assess the quantitative damage. Materials and methods: 30 smokers & 20 non smokers were recruited & their peripheral blood was taken for the comet assay to look for Olive moment & Tail moment to quantitatively assess the DNA damage due to cigarette smoking. Results: In our study there was no significant difference in the analysis of DNA damage (with regard to tail moment & olive moment) in smokers versus non smokers (P value: more than 0.05). Conclusions: Though smoking is known to cause DNA damage, we did not find significant differences between the two groups probably due to other multifactorial etiologies for genotoxicity.

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Protective effect of leaf extract of Abutilon indicum on DNA damage and peripheral blood lymphocytes in combating the oxidative stress

Saudi Pharmaceutical Journal ◽

10.1016/j.jsps.2020.06.015 ◽

2020 ◽

Vol 28 (8) ◽

pp. 943-950 ◽

Cited By ~ 1

Author(s):

Xingping Wu ◽

Sugapriya Dhanasekaran

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Protective Effect ◽

Peripheral Blood ◽

Leaf Extract ◽

Peripheral Blood Lymphocytes ◽

Blood Lymphocytes ◽

Abutilon Indicum

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Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes ExposedIn Vitroto Neonicotinoid Insecticides News

Journal of Toxicology ◽

10.1155/2012/612647 ◽

2012 ◽

Vol 2012 ◽

pp. 1-11 ◽

Cited By ~ 33

Author(s):

María Elena Calderón-Segura ◽

Sandra Gómez-Arroyo ◽

Rafael Villalobos-Pietrini ◽

Carmen Martínez-Valenzuela ◽

Yolanda Carbajal-López ◽

...

Keyword(s):

Dna Damage ◽

Peripheral Blood ◽

Human Peripheral Blood ◽

Peripheral Blood Lymphocytes ◽

Blue Dye ◽

Cytotoxic Effects ◽

Blood Lymphocytes ◽

Neonicotinoid Insecticides ◽

Human Peripheral Blood Lymphocytes

Calypso (thiacloprid), Poncho (clothianidin), Gaucho (imidacloprid), and Jade (imidacloprid) are commercial neonicotinoid insecticides, a new class of agrochemicals in México. However, genotoxic and cytotoxic studies have not been performed. In the present study, human peripheral blood lymphocytes (PBL) were exposedin vitroto different concentrations of the four insecticides. The genotoxic and cytotoxic effects were evaluated using the alkaline comet and trypan blue dye exclusion assays. DNA damage was evaluated using two genotoxicity parameters: tail length and comet frequency. Exposure to9.5×10-6to5.7×10-5 M Jade;2.8×10-4to1.7×10-3 M Gaucho;0.6×10-1to1.4×10-1 M Calypso;1.2×10-1to9.5×10-1 M Poncho for 2 h induced a significant increase DNA damage with a concentration-dependent relationship. Jade was the most genotoxic of the four insecticides studied. Cytotoxicity was observed in cells exposed to18×10-3 M Jade,2.0×10-3 M Gaucho,2.0×10-1 M Calypso, 1.07 M Poncho, and cell death occurred at30×10-3 M Jade,3.3×10-3 M Gaucho,2.8×10-1 M Calypso, and 1.42 M Poncho. This study provides the first report of genotoxic and cytotoxic effects in PBL followingin vitroexposure to commercial neonicotinoid insecticides.

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