Lecithin blended polyamide-6 high aspect ratio nanofiber scaffolds via electrospinning for human osteoblast cell culture

Abstract The aim of the present study was to test the hypothesis that the proliferation of a human osteoblast cell line (HOS cells) stimulated with hydroxyapatite (HA) may be regulated by nitric oxide (NO). The cells were cultured on the surface of HA. Medium or cells alone were used as controls. L-arginine, D-arginine, 7-NI (an nNOS inhibitor), L-NIL (an iNOS inhibitor), L-NIO (an eNOS inhibitor) or carboxy PTIO, a NO scavenger, was added in the HA-exposed cell cultures. The cells were also precoated with anti-human integrin αV antibody. The levels of nitrite were determined spectrophotometrically. Cell proliferation was assessed by colorimetric assay. The results showed increased nitrite production and cell proliferation by HA-stimulated HOS cells up to day 3 of cultures. Anti-integrin αV antibody, L-NIO, or carboxy PTIO suppressed, but L-arginine enhanced, nitrite production and cell proliferation of HA-stimulated HOS cells. The results of the present study suggest, therefore, that interaction between HA and HOS cell surface integrin αV molecule may activate eNOS to catalyze NO production which, in turn, may regulate the cell proliferation in an autocrine fashion.

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Nitric oxide production by a human osteoblast cell line stimulated withAggregatibacter actinomycetemcomitanslipopolysaccharide

Oral Microbiology and Immunology ◽

10.1111/j.1399-302x.2008.00475.x ◽

2009 ◽

Vol 24 (1) ◽

pp. 50-55 ◽

Cited By ~ 10

Author(s):

W. Sosroseno ◽

P. S. Bird ◽

G. J. Seymour

Keyword(s):

Nitric Oxide ◽

Cell Line ◽

Nitric Oxide Production ◽

Human Osteoblast ◽

Osteoblast Cell ◽

Human Osteoblast Cell ◽

Oxide Production ◽

Osteoblast Cell Line

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Growth hormone and insulin-like growth factorI have differential effects on osteoprotegerin and osteoprotegerin ligand gene expression in the human osteoblast cell line MG-63

Growth Hormone & IGF Research ◽

10.1016/s1096-6374(01)80053-8 ◽

2001 ◽

Vol 11 ◽

pp. S158

Author(s):

B.-J. Kim ◽

W.W. Jung ◽

M.-J. Kang ◽

K.-J. Ahn ◽

Y.-S. Joo

Keyword(s):

Gene Expression ◽

Growth Hormone ◽

Cell Line ◽

Human Osteoblast ◽

Osteoblast Cell ◽

Human Osteoblast Cell ◽

Differential Effects ◽

Osteoblast Cell Line ◽

Osteoprotegerin Ligand

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Vero Cell Viability and Human Osteoblast Cell Response to Electrospun Phase Controlled Titania Nanofibers

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2012.1053 ◽

2012 ◽

Vol 2 (4) ◽

pp. 292-298 ◽

Cited By ~ 6

Author(s):

A. Sandeep Kranthi Kiran ◽

Rajkamal Balu ◽

T. S. Sampath Kumar

Keyword(s):

Cell Viability ◽

Cell Response ◽

Human Osteoblast ◽

Osteoblast Cell ◽

Human Osteoblast Cell ◽

Titania Nanofibers

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Nanostructured Electrodes for Improved Neural Recording

MRS Proceedings ◽

10.1557/proc-729-u4.11 ◽

2002 ◽

Vol 729 ◽

Cited By ~ 4

Author(s):

Karen C. Cheung ◽

Yang-Kyu Choi ◽

Tim Kubow ◽

Luke P. Lee

Keyword(s):

Cell Culture ◽

Surface Roughness ◽

Aspect Ratio ◽

High Aspect Ratio ◽

Electrode Surface ◽

Neural Recording ◽

New Method ◽

Nanostructured Electrodes

AbstractWe present a new method of increasing the effective electrode surface for improved neural recording. To optimize the electrode, the impedance can be decreased by introducing surface roughness or nanostructures on the electrode. High aspect ratio pillar-like polysilicon nanostructures are created in a reactive ion etch. Nanostructure robustness in cell culture is examined.

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