Application of cold plasma corona discharge in preparation of laccase-based biosensors for dopamine determination

2020 ◽  
Vol 116 ◽  
pp. 111199
Author(s):  
Cecylia Wardak ◽  
Beata Paczosa-Bator ◽  
Szymon Malinowski
Keyword(s):  
Corona Discharge ◽  
Cold Plasma ◽  
Plasma Corona ◽  
Dopamine Determination

Use of Cold Plasma To Inactivate Escherichia coli and Physicochemical Evaluation in Pumpkin Puree

2018 ◽  
Vol 81 (11) ◽  
pp. 1897-1905 ◽  
Author(s):  
L. C. O. SANTOS ◽  
A. L. V. CUBAS ◽  
E. H. S. MOECKE ◽  
D. H. B. RIBEIRO ◽  
E. R. AMANTE
Keyword(s):  
Escherichia Coli ◽  
Corona Discharge ◽  
Cold Plasma ◽  
Treatment Time ◽  
Physicochemical Characteristics ◽  
Carotenoid Content ◽  
E Coli ◽  
Process Gas ◽  
Total Carotenoid Content ◽  
Plasma Corona

ABSTRACT This work aimed to study the pumpkin puree processing by cold plasma corona discharge as an alternative to heat treatment to reduce Escherichia coli contamination and evaluate physicochemical alterations, using argon (Ar) as the process gas. The treatment time to verify E. coli inactivation was between 2 and 20 min, while physicochemical alterations were analyzed at 5, 10, 15, and 20 min. Cold plasma corona discharge technology to inactivate E. coli proved to be promising, reaching 3.62 log cycles of reduction at 20 min of treatment. The inactivation kinectics showed a tendency of higher decrease with time. Physicochemical characteristics indicate that plasma induces a decrease of pH; however, there is an indication that process gases have an important role and react with the environment and procedure reactive species. This technology may reduce the total carotenoid content of pumpkin puree and in color, mainly the a* parameter, which showed great reduction.

scholarly journals Effects of Atmospheric Plasma Corona Discharge on Agrobacterium tumefaciens Survival

2021 ◽  
Vol 10 (1) ◽  
pp. 32
Author(s):  
Yulia Lazra ◽  
Bharath Gandu ◽  
Irina Dubrovin Amar ◽  
Efrat Emanuel ◽  
Rivka Cahan
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Corona Discharge ◽  
Membrane Permeability ◽  
Cell Size ◽  
Cold Plasma ◽  
Bacterial Eradication ◽  
Liquid Environment ◽  
Initial Concentration ◽  
Plasma Corona ◽  
The Impact

Soil-borne pathogenic microorganisms are known to cause extensive crop losses. Agrobacterium tumefaciens, a member of the Proteobacteria, causes the neoplastic crown gall disease in plants. Plant protection is mainly based on toxic chemicals that are harmful to the environment. The use of cold atmospheric-pressure plasma is an attractive method for microbial eradication. Its antimicrobial mechanism includes the formation of large quantities of reactive oxygen species (ROS). The advantages of eradicating bacteria using cold plasma are not needed for chemicals, short treatment, and environmental temperatures. This study examined the impact of plasma corona discharge exposure on A. tumefaciens viability, membrane permeability, relative cell size, and ROS formation. The results showed that 90 s of plasma exposure led to a reduction by four orders of magnitude when the initial concentration was 1 × 107 CFU/mL and in a dry environment. When the initial concentration was 1 × 106 CFU/mL, 45 s of exposure resulted in total bacterial eradication. In a liquid environment, in an initial concentration of 2.02 × 106 CFU/mL, there was no complete bacterial eradication even at the most prolonged examined exposure (90 s). The influence of plasma treatment on the membrane permeability of A. tumefaciens, and their possible recovery, were analyzed using flow cytometer analysis using propidium iodide (PI). When the plasma-treated bacteria were suspended in Luria–Bertani (LB) (rich medium), the PI-positive count of the plasma-treated bacteria after two hours was 12 ± 3.9%. At the 24th hour, this percentage was only 1.74 ± 0.6%, as the control (0.7 ± 0.1%). These results may indicate the repair of the plasma-treated bacteria that were suspended in LB. At the 24th hour, the relative cell size of the treated bacteria shifted to the right, to ~3 × 104 forward side scatter (FSC), about 0.5-fold higher than the untreated cells. Measurement of the ROS showed that the intracellular fluorescence of the 90-s plasma-treated cells led to significant fluorescence formation of 32 relative fluorescence units (RFU)/cell (9 × 104 fold, compared to the nontreated cells). This study showed that cold plasma is a useful method for A. tumefaciens eradication. The eradication mechanism involves ROS generation, membrane permeability, and changes in cell size.

Cold Plasma Inactivation of Salmonella in Prepackaged, Mixed Salads Is Influenced by Cross-Contamination Sequence

2017 ◽  
Vol 80 (12) ◽  
pp. 2132-2136 ◽  
Author(s):  
Sarah M. Hertrich ◽  
Glenn Boyd ◽  
Joseph Sites ◽  
Brendan A. Niemira
Keyword(s):  
Shelf Life ◽  
Corona Discharge ◽  
Cold Plasma ◽  
Food Products ◽  
Plate Count ◽  
Cross Contamination ◽  
Plastic Container ◽  
Peptone Water ◽  
Plasma Corona ◽  
Cherry Tomatoes

ABSTRACT Customer demand for convenient food products has led to an increased production of prepackaged and ready-to-eat food products. Most of these products rely mainly on surface disinfection and other traditional approaches to ensure shelf life and safety. Novel processing techniques, such as cold plasma, are currently being investigated to enhance the safety and shelf life of prepacked foods. The purpose of this study was to determine the effects of cold plasma corona discharge on the inactivation of Salmonella on prepackaged, tomato-and-lettuce mixed salads. Two different inoculation methods were evaluated to address cross-contamination of Salmonella from cherry tomatoes to lettuce and vice versa. In separate studies, a sample of either cherry tomatoes (55 g) or romaine lettuce (10 g) was inoculated with a Salmonella cocktail (6.93 ± 0.99 log CFU/mL), placed into a commercial polyethylene terephthalate plastic container, and thoroughly mixed together with its noninoculated counterpart. Mixed salads were allowed to dry in a biosafety cabinet for 1 h. Samples were treated with 35 kV cold plasma corona discharge inside plastic containers for 3 min. Samples were stomached and serially diluted in buffered peptone water and then were plated onto aerobic plate count Petrifilm and incubated for 18 h at 37°C. When lettuce was the inoculated counterpart, log kill of Salmonella was significantly greater on tomatoes (0.75 log CFU/g) compared with lettuce (0.34 log CFU/g) (P = 0.0001). Salmonella was reduced on mixed salad only when lettuce was the inoculated counterpart (0.29 log CFU/g) (P = 0.002). Cold plasma can kill Salmonella in a prepackaged mixed salad, with efficacy dependent on the nature of contamination, direction of transfer, and the surface topography of the contaminated commodity.

scholarly journals Organic and Bio Material Surface Modification Via Corona Discharge Induced Atmospheric-cold Plasma

2016 ◽  
Vol 86 ◽  
pp. 325-328 ◽  
Author(s):  
Natthaporn Khamsen ◽  
Aric Akkarachanchainon ◽  
Nithiphat Teerakawanich ◽  
Siwapon Srisonphan
Keyword(s):  
Surface Modification ◽  
Corona Discharge ◽  
Cold Plasma ◽  
Material Surface ◽  
Bio Material

scholarly journals Water sterilization using plasma corona discharge

2019 ◽  
Author(s):  
Kusumandari Kusumandari ◽  
Teguh Endah Saraswati ◽  
Rizki Ika Oktaviana ◽  
Khanza Fadhilah Almas ◽  
Haya Alvinesha Puspitadindha
Keyword(s):  
Corona Discharge ◽  
Plasma Corona

scholarly journals Atmospheric Cold Plasma via Fringe Field Enhanced Corona Discharge on Single Dielectric Barrier for Large-volume Applications

2016 ◽  
Vol 86 ◽  
pp. 321-324 ◽  
Author(s):  
Natthaporn Khamsen ◽  
Aric Akkarachanchainon ◽  
Kamonpun Fookiat ◽  
Jaraspong Srisala ◽  
Suvit Chomchuen ◽  
...  
Keyword(s):  
Corona Discharge ◽  
Large Volume ◽  
Cold Plasma ◽  
Fringe Field ◽  
Dielectric Barrier

scholarly journals Co oxidation over alumina monolith impregnated with oxides of copper and manganese

2021 ◽  
pp. 4-4
Author(s):  
Thien Pham ◽  
Viet Bui ◽  
Thi Phan ◽  
Ha Than
Keyword(s):  
Carbon Monoxide ◽  
Corona Discharge ◽  
Atmospheric Pressure ◽  
Oxide Catalyst ◽  
Simple Method ◽  
Wet Impregnation ◽  
Uniform Dispersion ◽  
Plasma Corona ◽  
And Performance ◽  
High Yields

In this work, preparation and performance of catalysts as using a simple method and highly efficient heterogeneous nanocatalyst is described. The main advantages of the reaction are high yields for the oxidation of CO at low temperature. The catalysts based-on oxides of copper and manganese supported on alumina monoliths were prepared by the different methods: plasma corona discharge and wet impregnation. Structure and physical properties of catalysts were characterized by FT-IR, XRD, TEM, EDX and TG/DTA. The results showed that the using of plasma corona discharge at atmospheric pressure in the preparation process of catalyst gives a smaller particle size, uniform dispersion when compared with the catalysts prepared by the wet impregnation methods. The catalytic activities of these catalysts were investigated for complete oxidation of carbon monoxide (3000 ppm) to carbon dioxide in the air at the atmospheric pressure. On a single oxide catalyst, 10CuO/monolith was better than 10MnO2/monolith in the same experimental condition. With multi-oxide catalysts, all catalyst samples are more active than a single-oxide catalyst in the same impregnated content. In particular, the catalyst is prepared by plasma corona discharge indicate the best oxidation capacity of carbon monoxide (CO).

Sign in / Sign up

Export Citation Format

Share Document