scholarly journals Corrigendum to “Influence of polymer molecular weight on the in vitro cytotoxicity of poly (N-isopropylacrylamide)” [Mater. Sci. Eng. C (2016) 509–513]

2021 ◽  
Vol 129 ◽  
pp. 112368
Author(s):  
Amir Mellati ◽  
Meisam Valizadeh Kiamahalleh ◽  
Sheng Dai ◽  
Jingxiu Bi ◽  
Bo Jin ◽  
...  
Keyword(s):  
Molecular Weight ◽  
In Vitro Cytotoxicity ◽  
Polymer Molecular Weight

Influence of polymer molecular weight on the in vitro cytotoxicity of poly (N-isopropylacrylamide)

2016 ◽  
Vol 59 ◽  
pp. 509-513 ◽  
Author(s):  
Amir Mellati ◽  
Meisam Valizadeh Kiamahalleh ◽  
Sheng Dai ◽  
Jingxiu Bi ◽  
Bo Jin ◽  
...  
Keyword(s):  
Molecular Weight ◽  
In Vitro Cytotoxicity ◽  
Polymer Molecular Weight

scholarly journals Physicochemical and Biological Examination of Two Glatiramer Acetate Products

Biomedicines ◽  
2019 ◽  
Vol 7 (3) ◽  
pp. 49 ◽  
Author(s):  
Komlosh ◽  
Weinstein ◽  
Loupe ◽  
Hasson ◽  
Timan ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Glatiramer Acetate ◽  
Active Ingredient ◽  
In Vitro Cytotoxicity ◽  
Exchange Chromatography ◽  
Size Exclusion ◽  
Potency Assay ◽  
Scientific Discussion

Herein we compared 40 mg/mL lots of the active ingredient, glatiramer acetate, manufactured by Mylan/Natco to the active ingredient, glatiramer acetate in Copaxone (Teva Pharmaceuticals, Ltd., Netanya Israel) using physicochemical (PCC) methods and biological assays. No differences were seen between the Mylan/Natco and Teva lots with some low resolution release PCC assays (amino acid analysis, molecular weight distribution, interaction with Coomassie Brilliant Blue G-250). Changes in polydispersity between Mylan/Natco and Copaxone lots were found using size exclusion chromatography and the high resolution PCC method, known as Viscotek, and suggestive of a disparity in the homogeneity of mixture, with a shift towards high molecular weight polypeptides. Using RPLC-2D MALLS, 5 out of 8 Mylan/Natco lots fell outside the Copaxone range, containing a high molecular weight and high hydrophobicity subpopulation of polypeptides not found in Copaxone lots. Cation exchange chromatography showed differences in the surface charge distribution between the Copaxone and Mylan/Natco lots. The Mylan/Natco lots were found to be within Copaxone specifications for the EAE model, monoclonal and polyclonal binding assays and the in vitro cytotoxicity assay, however higher IL-2 secretion was shown for three Mylan/Natco lots in a potency assay. These observations provide data to inform the ongoing scientific discussion about the comparability of glatiramer acetate in Copaxone and follow-on products.

Influence of Polymer Molecular Weight on in-vitro Characteristics and Cytotoxicity of Fulvestrant Loaded Nanoparticles

2015 ◽  
Vol 9 (4) ◽  
pp. 239-249 ◽  
Author(s):  
Canan Hascicek ◽  
Ceyda Sengel-Turk ◽  
Mehmet Gumustas ◽  
Sibel Ozkan ◽  
Filiz Bakar ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Polymer Molecular Weight

scholarly journals Chitosan-Mediated siRNA Delivery In Vitro: Effect of Polymer Molecular Weight, Concentration and Salt Forms

2010 ◽  
Vol 11 (1) ◽  
pp. 64-72 ◽  
Author(s):  
Sunee Techaarpornkul ◽  
Sirirat Wongkupasert ◽  
Praneet Opanasopit ◽  
Auayporn Apirakaramwong ◽  
Jurairat Nunthanid ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Sirna Delivery ◽  
Polymer Molecular Weight ◽  
Weight Concentration ◽  
Vitro Effect ◽  
Salt Forms

scholarly journals In vitro cytotoxicity, in vivo pharmacokinetic studies and tissue distribution studies of multifunctional citric acid dendrimers using the drug Cytarabine

2017 ◽  
Vol 9 (2) ◽  
pp. 28
Author(s):  
K Ravindra Reddy ◽  
B Narasimha Rao ◽  
KB Chandra Sekhar
Keyword(s):  
Molecular Weight ◽  
Citric Acid ◽  
High Surface ◽  
In Vitro Cytotoxicity ◽  
Pharmacokinetic Parameters ◽  
Pharmacokinetic Studies ◽  
Acute Cytotoxicity ◽  
Distribution Studies

<p>Dendrimers are considered the emerging polymeric architectures, known for their well defined molecular-weight, polydispersity, uniformity and high-surface functionality. These nano-architectures are capable of encapsulating low-high molecular-weight drug moieties in their interior or exterior through covalent bonding and host-guest interactions. Further, large surface volume made researchers to implicate dendrimers in biomedical and therapeutic applications. Regardless of the massive applications, sometimes its use is limited because of the cytotoxicity produced.  Considering this, the present research is focused on the synthesis and PEGylation of citric acid dendrimers. PEGylation is an act of conjugating polyethylene glycol to dendrimers that completely eliminates the toxicity issues associated with dendrimers and render them biocompatible. Cytarabine was loaded in the dendritic architecture to target specifically the tumor cells. Dendrimers are made tumor specific by incorporating certain agents that get cleaved in tumor environment. Synthesized dendrimers were studied for its effect on acute cytotoxicity, tissue-distributions and pharmacokinetic parameters.</p>

Relevance of polymer molecular weight to the in vitro/in vivo performances of ocular inserts based on poly(ethylene oxide)

2001 ◽  
Vol 220 (1-2) ◽  
pp. 169-177 ◽  
Author(s):  
G Di Colo ◽  
S Burgalassi ◽  
P Chetoni ◽  
M.P Fiaschi ◽  
Y Zambito ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Ethylene Oxide ◽  
Polymer Molecular Weight ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene

scholarly journals Star Polymer-Drug Conjugates with pH-Controlled Drug Release and Carrier Degradation

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
H. Kostková ◽  
L. Schindler ◽  
L. Kotrchová ◽  
M. Kovář ◽  
M. Šírová ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Star Polymers ◽  
Controlled Drug Release ◽  
Blood Stream ◽  
In Vitro Cytotoxicity ◽  
Star Polymer ◽  
Molar Ratio ◽  
Design Synthesis ◽  
Dendritic Core

In this study, we describe the design, synthesis, and physicochemical and preliminary biological characteristics of new biodegradable, high-molecular-weight (HMW) drug delivery systems with star-like architectures bearing the cytotoxic drug doxorubicin (DOX) attached by a hydrazone bond-containing spacer. The star polymers were synthesized by grafting semitelechelic N-(2-hydroxypropyl) methacrylamide (HPMA) copolymers on a 2,2-bis(hydroxymethyl)propionic acid- (bis-MPA-) based polyester dendritic core. The molecular weight of the star polymers ranged from 280 to 450 000 g/mol and could be adjusted by proper selection of the bis-MPA dendrimer generation and by considering the polymer to dendrimer molar ratio. The biodegradation of the polymer conjugates is based on the spontaneous slow hydrolysis of the dendritic core in neutral physiological conditions. Hydrazone spacers in the conjugates were fairly stable at neutral pH (7.4) mimicking blood stream conditions, and DOX was released from the conjugates under mild acidic conditions simulating the tumor cell microenvironment in endosomes and lysosomes (pH 5). Finally, we have shown the significant in vitro cytotoxicity of the star polymer-DOX conjugate on selected cancer cell lines with IC50 values almost comparable with that of the free drug and higher than that observed for a linear polymer-DOX conjugate with much lower molecular weight.

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