scholarly journals Fabrication of heparinized small diameter TPU/PCL bi-layered artificial blood vessels and in vivo assessment in a rabbit carotid artery replacement model

2021 ◽  
pp. 112628
Author(s):  
Zhiping Fang ◽  
Yonghao Xiao ◽  
Xue Geng ◽  
Liujun Jia ◽  
Yuehao Xing ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Blood Vessels ◽  
Small Diameter ◽  
Artificial Blood ◽  
Rabbit Carotid Artery ◽  
Artificial Blood Vessels ◽  
Replacement Model ◽  
In Vivo Assessment

Control of weft yarn or density improves biocompatibility of PET small diameter artificial blood vessels

2017 ◽  
Vol 106 (3) ◽  
pp. 954-964 ◽  
Author(s):  
Xingyou Hu ◽  
Tao Hu ◽  
Guoping Guan ◽  
Shaoting Yu ◽  
Yufen Wu ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Small Diameter ◽  
Weft Yarn ◽  
Artificial Blood ◽  
Artificial Blood Vessels

Electrostatic Self-Assemble Modified Electrospun Poly-L-Lactic Acid/Poly-Vinylpyrrolidone Composite Polymer and Its Potential Applications in Small-Diameter Artificial Blood Vessels

2020 ◽  
Vol 16 (1) ◽  
pp. 101-110 ◽  
Author(s):  
Fei Xu ◽  
Yubo Fan
Keyword(s):  
Lactic Acid ◽  
Blood Vessels ◽  
Photoelectron Spectroscopy ◽  
Laser Scanning ◽  
Electrospun Fiber ◽  
Average Molecular Weight ◽  
Small Diameter ◽  
Ph Change ◽  
Artificial Blood ◽  
Artificial Blood Vessels

Large (> 6 mm) artificial blood vessels have been successfully employed in clinic practice. However, small-diameter (< 6 mm) synthetic grafts have not been applied due to their hydrophobicity. In this study, poly-vinylpyrrolidone (PVP) was introduced into poly-L-lactic acid (PLLA) to prepare biodegradable small-diameter electrospun blood vessels which were further modified via electrostatic self-assembly (ESA). The characteristics of PLLA/PVP films were investigated by scanning electron microscopy (SEM), contact angle measurement and mechanical property testing. The cytocompatiblility and blood compatibility of the fiber films were further studied through vascular smooth muscle cells (VSMCs) proliferation and platelet adhesion, and the morphology of cells on films was viewed by laser scanning confocal microscopy (LSCM) and SEM. Next, the surface of ESA-modified electrospun fiber films was analyzed through SEM and photoelectron spectroscopy (XPS). The degradation characteristics of these films were investigated through SEM observation, weight loss, viscosity average molecular weight reduction, and pH change in the testing solutions as well. The films were also subcutaneously implanted in rabbits to analyze the biocompatibility. The results of these experiments showed that electrospun films with PVP possessed a good structure and improved hydrophilicity. The films assembled with chitosan/heparin by ESA were beneficial to VSMCs survival and had excellent blood compatibilities. The data indicated these films were biodegradable with good tissue compatibility. In conclusion, we successfully obtained biodegradable small-diameter blood vessels through electrospinning PLLA/PVP and modifying this blend's surface using ESA. The study provided a feasible method for making small-diameter synthetic blood vessels with improved hydrophilic and anticoagulant properties.

THE ADHESION OF LEUKOCYTES, ERYTHROCYTES, AND NONCELLULAR MATERIAL TO THE LUMINAL SURFACE OF NATURAL AND ARTIFICIAL BLOOD VESSELS IN VIVO

1977 ◽  
Vol 283 (1 The Behavior) ◽  
pp. 179-207 ◽  
Author(s):  
Gwendolyn J. Stewart ◽  
Peter R. Lynch ◽  
Frederick A. Reichle ◽  
William G. M. Ritchie ◽  
Allen Smith ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Luminal Surface ◽  
Artificial Blood ◽  
Artificial Blood Vessels

Abstract 245: Non-Invasive in vivo Assessment of the Re-Endothelialization Process Using Ultrasound Biomicroscopy in the Rat Carotid Artery Balloon Injury Model

2017 ◽  
Vol 37 (suppl_1) ◽  
Author(s):  
Samuel Röhl ◽  
Linnea Eriksson ◽  
Robert Saxelin ◽  
Mariette Lengquist ◽  
Kenneth Caidahl ◽  
...  
Keyword(s):  
Carotid Artery ◽  
High Frequency ◽  
Ultrasound Biomicroscopy ◽  
Blue Dye ◽  
High Frequency Ultrasound ◽  
Balloon Injury ◽  
En Face ◽  
In Vivo Assessment ◽  
Frequency Ultrasound

Objective: Ultrasound BioMicroscopy (UBM), or high-frequency ultrasound, is a novel technique used for assessment of anatomy and physiology small research animals. In this study, we evaluate the UBM assessment of the re-endothelialization process following denudation of the carotid artery in rats. Methods: Ultrasound BioMicroscopy data from three different experiments were analyzed. A total of 66 rats of three different strains (Sprague-Dawley, Wistar and Goto-Kakizaki) were included in this study. All animals were subjected to common carotid artery balloon injury and examined with UBM 2 and 4 weeks after injury. Re-endothelialization in UBM was measured as the length from the carotid bifurcation to the distal edge of the intimal hyperplasia. En face staining with Evans-blue dye was performed upon euthanization at 4 weeks after injury followed by tissue harvest for morphological and immunohistochemical evaluation. Results: A significant correlation (Spearman r=0.63,p<0.0001) and an agreement according to Bland-Altman test was identified when comparing all measurements of re-endothelialization in high frequency ultrasound and en face staining. Analysis by animal strain revealed a similar pattern and a significant growth in re-endothelialization length measured in UBM from 2 to 4 weeks could be identified. Immunohistochemical staining for von Willebrand factor confirmed the presence of endothelium in the areas detected as re-endothelialized by the ultrasound assessment. Conclusion: Ultrasound BioMicroscopy can be used for longitudinal in vivo assessment of the re-endothelialization following arterial injury in rats.

Scaffolds in tissue engineering of blood vessels

2010 ◽  
Vol 88 (9) ◽  
pp. 855-873 ◽  
Author(s):  
Divya Pankajakshan ◽  
Devendra K. Agrawal
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Small Diameter ◽  
Biological Scaffolds ◽  
Hemodynamic Forces ◽  
Biodegradable Scaffolds

Tissue engineering of small diameter (<5 mm) blood vessels is a promising approach for developing viable alternatives to autologous vascular grafts. It involves in vitro seeding of cells onto a scaffold on which the cells attach, proliferate, and differentiate while secreting the components of extracellular matrix that are required for creating the tissue. The scaffold should provide the initial requisite mechanical strength to withstand in vivo hemodynamic forces until vascular smooth muscle cells and fibroblasts reinforce the extracellular matrix of the vessel wall. Hence, the choice of scaffold is crucial for providing guidance cues to the cells to behave in the required manner to produce tissues and organs of the desired shape and size. Several types of scaffolds have been used for the reconstruction of blood vessels. They can be broadly classified as biological scaffolds, decellularized matrices, and polymeric biodegradable scaffolds. This review focuses on the different types of scaffolds that have been designed, developed, and tested for tissue engineering of blood vessels, including use of stem cells in vascular tissue engineering.

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