Preparation of nitric oxide-releasing photo-crosslinked electrospun chitosan nanofibrous scaffolds for bone tissue engineering

AbstractIn the previous studies, we have successfully developed a novel spiral structured nanofibrous scaffolds with improved osteoconductivity for bone tissue engineering. The spiral structure design facilitates the nutrient transport and waste removal, and allows uniform cellular growth and distribution within the scaffolds, thus enhanced the bioactivity of the scaffolds. In this chapter, HAP and BMP-2 were incorporated within the nanofibrous spiral scaffolds in order to enhance the osteoinductivity of the established system. The effect of the blending materials was evaluated through cell proliferation, cell differentiation of human osteoblast cells seeded on the scaffolds and cultured for 4 and 8 days. The results has demonstrated that the functionalization of PCL nanofibrous spiral scaffolds leads to higher ALP expression level and increased amount of mineralization level however lower cell proliferation rate.

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A bird's eye view on the use of electrospun nanofibrous scaffolds for bone tissue engineering: Current state‐of‐the‐art, emerging directions and future trends

Nanomedicine Nanotechnology Biology and Medicine ◽

10.1016/j.nano.2016.05.014 ◽

2016 ◽

Vol 12 (7) ◽

pp. 2181-2200 ◽

Cited By ~ 72

Author(s):

Zahra Rezvani ◽

Jayarama R. Venugopal ◽

Aleksandra M. Urbanska ◽

David K. Mills ◽

Seeram Ramakrishna ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

State Of The Art ◽

Future Trends ◽

Current State ◽

Nanofibrous Scaffolds

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Controlled degradability of PCL-ZnO nanofibrous scaffolds for bone tissue engineering and their antibacterial activity

Materials Science and Engineering C ◽

10.1016/j.msec.2018.08.009 ◽

2018 ◽

Vol 93 ◽

pp. 724-738 ◽

Cited By ~ 21

Author(s):

Betiana Felice ◽

María Alejandra Sánchez ◽

María Cecilia Socci ◽

Luciano David Sappia ◽

María Inés Gómez ◽

...

Keyword(s):

Tissue Engineering ◽

Antibacterial Activity ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Nanofibrous Scaffolds

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Is There a Governing Role of Osteocytes in Bone Tissue Regeneration?

Current Osteoporosis Reports ◽

10.1007/s11914-020-00610-6 ◽

2020 ◽

Vol 18 (5) ◽

pp. 541-550

Author(s):

Wei Cao ◽

Marco N. Helder ◽

Nathalie Bravenboer ◽

Gang Wu ◽

Jianfeng Jin ◽

...

Keyword(s):

Nitric Oxide ◽

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Regeneration ◽

Bone Tissue ◽

Bone Remodeling ◽

Tissue Regeneration ◽

Bone Cells ◽

Bone Tissue Regeneration ◽

Osteoblast Activity

Abstract Purpose of Review Bone regeneration plays an important role in contemporary clinical treatment. Bone tissue engineering should result in successful bone regeneration to restore congenital or acquired bone defects in the human skeleton. Osteocytes are thought to have a governing role in bone remodeling by regulating osteoclast and osteoblast activity, and thus bone loss and formation. In this review, we address the so far largely unknown role osteocytes may play in bone tissue regeneration. Recent Findings Osteocytes release biochemical signaling molecules involved in bone remodeling such as prostaglandins, nitric oxide, Wnts, and insulin-like growth factor-1 (IGF-1). Treatment of mesenchymal stem cells in bone tissue engineering with prostaglandins (e.g., PGE2, PGI2, PGF2α), nitric oxide, IGF-1, or Wnts (e.g., Wnt3a) improves osteogenesis. Summary This review provides an overview of the functions of osteocytes in bone tissue, their interaction with other bone cells, and their role in bone remodeling. We postulate that osteocytes may have a pivotal role in bone regeneration as well, and consequently that the bone regeneration process may be improved effectively and rapidly if osteocytes are optimally used and stimulated.

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Recent Developments in Nanofiber Fabrication and Modification for Bone Tissue Engineering

International Journal of Molecular Sciences ◽

10.3390/ijms21010099 ◽

2019 ◽

Vol 21 (1) ◽

pp. 99 ◽

Cited By ~ 8

Author(s):

Nopphadol Udomluck ◽

Won-Gun Koh ◽

Dong-Jin Lim ◽

Hansoo Park

Keyword(s):

Tissue Engineering ◽

Growth Factors ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Bone Cells ◽

Potential Candidate ◽

Cell Functions ◽

Nanofibrous Scaffolds ◽

Essential Components ◽

Post Modification

Bone tissue engineering is an alternative therapeutic intervention to repair or regenerate lost bone. This technique requires three essential components: stem cells that can differentiate into bone cells, growth factors that stimulate cell behavior for bone formation, and scaffolds that mimic the extracellular matrix. Among the various kinds of scaffolds, highly porous nanofibrous scaffolds are a potential candidate for supporting cell functions, such as adhesion, delivering growth factors, and forming new tissue. Various fabricating techniques for nanofibrous scaffolds have been investigated, including electrospinning, multi-axial electrospinning, and melt writing electrospinning. Although electrospun fiber fabrication has been possible for a decade, these fibers have gained attention in tissue regeneration owing to the possibility of further modifications of their chemical, biological, and mechanical properties. Recent reports suggest that post-modification after spinning make it possible to modify a nanofiber’s chemical and physical characteristics for regenerating specific target tissues. The objectives of this review are to describe the details of recently developed fabrication and post-modification techniques and discuss the advanced applications and impact of the integrated system of nanofiber-based scaffolds in the field of bone tissue engineering. This review highlights the importance of nanofibrous scaffolds for bone tissue engineering.

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