Identification of emergent blaCMY-2-carrying Proteus mirabilis lineages by whole-genome sequencing

Proteus mirabilis is a component of the normal intestinal microflora of humans and animals, but can cause urinary tract infections and even sepsis in hospital settings. In recent years, the number of multidrug-resistant P. mirabilis isolates, including the ones producing extended-spectrum β-lactamases (ESBLs), is increasing worldwide. However, the number of investigations dedicated to this species, especially, whole-genome sequencing, is much lower in comparison to the members of the ESKAPE pathogens group. This study presents a detailed analysis of clinical multidrug-resistant ESBL-producing P. mirabilis isolate using short- and long-read whole-genome sequencing, which allowed us to reveal possible horizontal gene transfer between Klebsiella pneumoniae and P. mirabilis plasmids and to locate the CRISPR-Cas system in the genome together with its probable phage targets, as well as multiple virulence genes. We believe that the data presented will contribute to the understanding of antibiotic resistance acquisition and virulence mechanisms for this important pathogen.

Download Full-text

Characterization of SXT/R391 Integrative and Conjugative Elements in Proteus mirabilis Isolates from Food-Producing Animals in China

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02852-15 ◽

2016 ◽

Vol 60 (3) ◽

pp. 1935-1938 ◽

Cited By ~ 25

Author(s):

Chang-Wei Lei ◽

An-Yun Zhang ◽

Hong-Ning Wang ◽

Bi-Hui Liu ◽

Li-Qin Yang ◽

...

Keyword(s):

Escherichia Coli ◽

Whole Genome Sequencing ◽

Genome Sequencing ◽

Resistance Genes ◽

Proteus Mirabilis ◽

Whole Genome ◽

Content Type ◽

Integrative And Conjugative Elements ◽

Animal Farms

SXT/R391 integrative and conjugative elements (ICEs) were detected in 8 out of 125Proteus mirabilisisolates from food-producing animals in China. Whole-genome sequencing revealed that seven ICEs were identical to ICEPmiJpn1, carrying the cephalosporinase geneblaCMY-2. Another one, designated ICEPmiChn1, carried five resistance genes. All eight ICEs could be transferred toEscherichia colivia conjugation. The results highlight the idea that animal farms are important reservoir of the SXT/R391 ICE-containingP. mirabilis.

Download Full-text

High prevalence of OXA-23 carbapenemase-producing Proteus mirabilis among amoxicillin-clavulanate resistant isolates in France

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01983-21 ◽

2021 ◽

Author(s):

Amélie Lombes ◽

Rémy A. Bonnin ◽

Frédéric Laurent ◽

Hélène Guet-Revillet ◽

Emmanuelle Bille ◽

...

Keyword(s):

Whole Genome Sequencing ◽

Genome Sequencing ◽

Proteus Mirabilis ◽

Inhibition Zone ◽

Whole Genome ◽

Multicentric Study ◽

Inhibition Zone Diameter ◽

Zone Diameter ◽

High Prevalence ◽

Amoxicillin Clavulanate

In this multicentric study performed in 12 French hospitals, we reported that 26.9% (14/52) of the amoxicillin/clavulanate-resistant Proteus mirabilis isolates produced the OXA-23 carbapenemase. We found that inhibition zone diameter less than 11 mm around amoxicillin/clavulanate disc was an accurate screening cut-off to detect these OXA-23 producers. We confirmed by whole genome sequencing that these OXA-23-producers all belonged to the same lineage that has been demonstrated to disseminate OXA-23 or OXA-58 in P. mirabilis .

Download Full-text