Association of the FTO (rs9939609) and MC4R (rs17782313) gene polymorphisms with maternal body weight during pregnancy

MC4R, LEP, and LEPR genes are involved in the hypothalamic leptin-melanocortin regulation pathway, which is important for energy homeostasis. Our study aimed to evaluate the associations between the MC4R rs17782313, LEP rs7799039, and LEPR rs1137101 polymorphisms with obesity-related parameters in childhood and adulthood. The data were obtained from the Kaunas Cardiovascular Risk Cohort study, which started in 1977 with 1082 participants aged 12–13 years. In 2012–2014, the follow-up survey was carried out. Genotype analysis of all respondents (n = 509) aged 48–49 years was performed for the gene polymorphisms using Real-Time Polymerase Chain Reaction. Anthropometric measurements were performed in childhood and adulthood. In childhood, only skinfold thicknesses were associated with gene variants being the lowest in children with MC4R TT genotype and LEP AG genotype. In adulthood, odds of obesity and metabolic syndrome was higher in MC4R CT/CC genotype than TT genotype carriers (OR 1.8; 95% CI 1.2–2.8 and OR 1.6; 95% CI 1.1–2.4, respectively). In men, physical activity attenuated the effect of the MC4R rs17782313 on obesity. The LEP GG genotype was associated with higher BMI, waist circumference, and visceral fat level only in men. No associations of the LEPR rs1137101 polymorphisms with anthropometric measurements and leptin level were found. In conclusion, the associations of the MC4R and LEP gene polymorphisms with obesity-related parameters strengthened with age.

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Association of maternal breast milk and serum levels of macronutrients, hormones, and maternal body composition with infant’s body weight

European Journal of Clinical Nutrition ◽

10.1038/s41430-017-0022-9 ◽

2017 ◽

Vol 72 (3) ◽

pp. 394-400 ◽

Cited By ~ 3

Author(s):

Adeleh Khodabakhshi ◽

Hassan Mehrad-Majd ◽

Farhad Vahid ◽

Mohammad Safarian

Keyword(s):

Body Composition ◽

Body Weight ◽

Breast Milk ◽

Serum Levels ◽

Maternal Body

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Combined effects of FTO rs9939609 and MC4R rs17782313 on elevated nocturnal blood pressure in the Chinese Han population

CardioVascular Journal of Africa ◽

10.5830/cvja-2015-064 ◽

2016 ◽

Vol 27 (1) ◽

pp. 21-24 ◽

Cited By ~ 4

Author(s):

Y Sun ◽

J Sun ◽

J Wu ◽

M Yang

Keyword(s):

Blood Pressure ◽

Chinese Han Population ◽

Combined Effects ◽

Chinese Han ◽

Han Population ◽

Nocturnal Blood Pressure ◽

Mc4r Rs17782313 ◽

Fto Rs9939609

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Effects of Propolis Extract Supplementation during Pregnancy on Stress Oxidative and Pregnancy Outcome: Levels of Malondialdehyde, 8-Oxo-2′-Deoxogunosine, Maternal Body Weight, and Number of Fetuses

Jurnal Kedokteran Brawijaya ◽

10.21776/ub.jkb.2021.031.03.5 ◽

2021 ◽

Vol 31 (3) ◽

pp. 156

Author(s):

Joko Wahyu Wibowo ◽

Minidian Fasitasari ◽

Siti Thomas Zulaikhah

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Pregnancy Outcomes ◽

Feeding Tube ◽

Control Group ◽

Maternal Body ◽

Control Group Design ◽

Pregnant Rats ◽

Propolis Extract ◽

Group I

<p>Oxidative stress is related to pregnancy complications that could increase maternal and infant mortality. This study aimed to determine the effect of propolis extract supplementation during pregnancy on oxidative stress level and pregnancy outcomes utilizing Malonedealdehyde (MDA) and 8-Oxo-2′-Deoxogunosine (8-OHdG) levels, maternal body weight, and the average number of fetuses as the parameters. The study was conducted by using a posttest only control group design on 24 pregnant Wistar rats, which were divided into four groups. Group I was control, Group II-IV were the treatment groups given propolis extract of 1.8mg, 3.6mg, and 7.2mg/200gBW/day, respectively. The standard feed given was AIN93G dose of 20g/day and distilled water ad libitum. Propolis extract was given using a gastric feeding tube every morning for 20 days. At the end of the treatment, body weight was meisured and blood collected for assessed MDA and 8-OHdG levels by ELISA method and then we performed abdominal surgery to count number of fetuses. The result are there were decreasing level of MDA and 8-OHDG by administration of propolis significantly (p<0.05) group: I: 2,04±0,091, II: 1,55±0,067, III: 1,05±0,176, IV: 0,73±0,075 (mmol/mL) (p=0.001); 8 OHdG level (ng/mL) group I: 10,02±0,403, II: 8,60±0,078, III: 7,89±0,051, IV: 7,53±0,063 (p=0,001). Average of maternal body weight (g) were increased: group I: 228,33±3,93, II: 237,17±4,36, III: 244,83±4,02, IV: 248,00±5,76 (p=0,001) and Average number of fetuses tend to increased as well, group I : 8,5±0,05, II: 7,8±0,41, III: 9,5±1,05, IV: 9,6±0,52 (p=0,02). The conclusion of this research are supplementation of propolis extract in pregnant rats can reduce oxidative stress and improve pregnancy outcomes.</p>

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Polymorphisms in the Chicken Growth Differentiation Factor 9 Gene Associated with Reproductive Traits

BioMed Research International ◽

10.1155/2018/9345473 ◽

2018 ◽

Vol 2018 ◽

pp. 1-11 ◽

Cited By ~ 3

Author(s):

Lingbin Liu ◽

Zhifu Cui ◽

Qihai Xiao ◽

Haihan Zhang ◽

Xiaoling Zhao ◽

...

Keyword(s):

Body Weight ◽

Single Nucleotide Polymorphisms ◽

Gene Polymorphisms ◽

Reproductive Traits ◽

Missense Mutations ◽

Nucleotide Polymorphisms ◽

Rt Pcr ◽

Single Nucleotide ◽

Egg Weight ◽

Growth Differentiation Factor 9

The aim of the study was to investigateGDF9gene polymorphisms and their association with reproductive traits in chicken using DNA sequencing. A total of 279 Dongxiang blue-shelled (DX) chickens and 232 Luhua (LH) chickens were used for validation. We detected 15 single nucleotide polymorphisms (SNPs): nine SNPs were previously unreported in chicken, two were missense mutations, and only three exhibited significant associations with reproductive traits. G.17156387C>T was significantly associated with age at first egg (AFE) and weight of first egg (WFE) in both breeds. Birds carrying the CC genotype exhibited higher AFE and WFE values than those with the TT genotype. The SNP g.17156427A>G exhibited an association with egg weight at 300 days of age (EWTA) in DX but not in LH chickens. The SNP g.17156703A>C affected the AFE and EN (total number of eggs at 300 days of age) in DX chickens. In addition, certain diplotypes significantly affected AFE, BWTA (body weight at 300 days of age), and EN in both breeds. RT-PCR results showed that theGDF9gene was highly expressed in stroma with cortical follicles (STR) and prehierarchal follicles. These results provided further evidence that theGDF9gene is involved in determining reproductive traits in chicken.

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Relationships between Hha1 Calpastatin Gene Polymorphism, Growth Performance, and Meat Characteristics of Awassi Sheep

Animals ◽

10.3390/ani9090667 ◽

2019 ◽

Vol 9 (9) ◽

pp. 667

Author(s):

Khaleel I. Jawasreh ◽

Ahmad H. Al-Amareen ◽

Pauline Y. Aad

Keyword(s):

Body Weight ◽

Growth Performance ◽

Meat Quality ◽

Gene Polymorphisms ◽

Carcass Characteristics ◽

Quality Parameters ◽

Longissimus Muscle ◽

Final Body Weight ◽

Awassi Sheep ◽

Ram Lambs

Advances in molecular genetics have allowed the identification of genes that can enhance livestock production. The aim of this study was to investigate possible relationships between the calpastatin (CAST) Hha1 gene polymorphisms and growth performance, carcass characteristics, and meat quality in Awassi sheep. A total of 87 blood samples were collected from two-week-old Awassi ram lambs. The amplification of the CAST Hha1 gene yielded a fragment of 622 bp. Three CAST genotypes were found in Awassi sheep: MM for two fragments (385 bp and 281 bp), MN for three fragments (622 bp, 385 bp, and 281 bp), and NN for only one fragment (622 bp). The M and N allele frequencies of the CAST Hha1 genotypes were 0.765 and 0.235, respectively, while the genotypic frequencies of MM, MN, and NN were 0.586, 0.356, and 0.057, respectively. Based on CAST Hha1 gene polymorphisms, three groups of lambs (MM: n = 8; MN: n = 6; and NN: n = 3 genotypes) were subjected to a fattening period of 70 days to investigate growth performance and meat characteristics. Only the final body weight and longissimus muscle width were significantly different between the three genotypes, while no significant differences were detected in any other carcass characteristics and meat quality parameters. In this study, new variants were observed in CAST using the Hha1 restriction site, potentially assisting in Awassi sheep breeding and selection programs to improve final body weight and longissimus muscle width.

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