Involvement of Toxoplasma gondii in reproductive disorders in Swiss pig farms

Toxoplasma gondii infection has been diagnosed in pigs all over the world. Economical losses are generally related to reproductive disorders. Toxoplasma infection is also a matter of public health because tissue cysts of the parasite may remain in pork and pork products, and become sources of human infection. The objective of this study was to evaluate the frequency and risk factors associated with Toxoplasma infection in certified and non-certified pig breeding farms in the Toledo microregion, in the State of Paraná, Brazil which includes the cities of Toledo, Nova Santa Rosa, Sao José das Palmeiras and Sao Pedro do Iguaçu. Relative frequency of infection was 13.4%, independently of the type of farm. Logistic regression analysis showed that the following factors were associated with infection: absence of workers exclusive for each area of the farm, access of other animals to feeders and drinkers, lack of lids in drinkers, lack of rodent control measures, mean piglet number and weight at weaning per female.

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Transcriptome Analysis of Testes and Uterus: Reproductive Dysfunction Induced by Toxoplasma gondii in Mice

Microorganisms ◽

10.3390/microorganisms8081136 ◽

2020 ◽

Vol 8 (8) ◽

pp. 1136

Author(s):

Junjie Wang ◽

Tanghui Liu ◽

Yasser S. Mahmmod ◽

Zipeng Yang ◽

Jiexing Tan ◽

...

Keyword(s):

Gene Ontology ◽

Toxoplasma Gondii ◽

Acute Infection ◽

The Other ◽

Rna Seq ◽

Mice Model ◽

Reproductive Disorders ◽

Kegg Database ◽

Disease Pathogenesis ◽

Differently Expressed Genes

Toxoplasma gondii (T. gondii) infection in female mammals during pregnancy can result in poor pregnancy. Similarly, it can result in male reproductive disorders in male mammals. Although the testes and uterus have very different biological makeup, they are still both attacked by T. gondii resulting in reproductive dysfunctions. We hypothesized that there are significant common genes in the testes and uterus that interact with T. gondii. Finding out and studying these genes is vital to understand the infection mechanism of T. gondii and the induced disease pathogenesis. To achieve this goal, we built a mice model of acute infection with T. gondii and the testes and uterus of the mice were sequenced by RNA-Seq. A total of 291 and 679 significantly differently expressed genes (DEGs) were obtained from the testes and the uterus, respectively. In the Gene Ontology (GO) analysis, part of the DEGs in the testes and uterus were related to 35 GO functions. When compared with the KEGG database, seven pathways affecting both the testes and uterus during the course of T. gondii infection were identified. In addition, Toxoplasmosis can significantly affect the expression of Nlrp5 and Insc leading to negative outcomes in the host. On the other hand, the host regulates Gbp7, Gbp2b, and Ifit3 to defend against T. gondii infection.

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Seroepidemiological and biomolecular survey on Toxoplasma gondii infection on organic pig farms

Parasitology Research ◽

10.1007/s00436-018-5823-9 ◽

2018 ◽

Vol 117 (5) ◽

pp. 1637-1641 ◽

Cited By ~ 7

Author(s):

A. P. Pipia ◽

A. Varcasia ◽

G. Dessì ◽

R. Panzalis ◽

C. Gai ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Pig Farms ◽

Toxoplasma Gondii Infection

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Infection dynamic of Toxoplasma gondii in two fattening pig farms exposed to high and low cat density in an endemic region

Veterinary Parasitology ◽

10.1016/j.vetpar.2010.10.018 ◽

2011 ◽

Vol 175 (3-4) ◽

pp. 367-371 ◽

Cited By ~ 21

Author(s):

A. Ortega-Pacheco ◽

K.Y. Acosta-Viana ◽

E. Guzman-Marin ◽

B. Uitzil-Álvarez ◽

J.C. Rodríguez-Buenfil ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Endemic Region ◽

Infection Dynamic ◽

Pig Farms

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Seroprevalence and risk factors associated with Toxoplasma gondii infection in pig farms from Catalonia, north-eastern Spain

Research in Veterinary Science ◽

10.1016/j.rvsc.2010.01.017 ◽

2010 ◽

Vol 89 (1) ◽

pp. 85-87 ◽

Cited By ~ 36

Author(s):

I. García-Bocanegra ◽

J.P. Dubey ◽

M. Simon-Grifé ◽

O. Cabezón ◽

J. Casal ◽

...

Keyword(s):

Risk Factors ◽

Toxoplasma Gondii ◽

Factors Associated ◽

North Eastern ◽

Pig Farms ◽

Toxoplasma Gondii Infection

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Coxiella burnetii associated with BVDV (Bovine Viral Diarrhea Virus), BoHV (Bovine Herpesvirus), Leptospira spp., Neospora caninum, Toxoplasma gondii and Trypanosoma vivax in reproductive disorders in cattle

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612019032 ◽

2019 ◽

Vol 28 (2) ◽

pp. 245-257 ◽

Cited By ~ 4

Author(s):

Diego Carlos Souza Zanatto ◽

Igor Renan Honorato Gatto ◽

Marcelo Bahia Labruna ◽

Marcia Mariza Gomes Jusi ◽

Samir Issa Samara ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Coxiella Burnetii ◽

Neospora Caninum ◽

Fluorescent Antibody ◽

Enzyme Linked Immunosorbent Assay ◽

Trypanosoma Vivax ◽

Reproductive Disorders ◽

Cross Sectional ◽

Diarrhea Virus ◽

Leptospira Spp

Abstract This is a cross-sectional study to assess the presence of antibodies in ruminants against selected pathogens associated with reproductive disorders in cattle in four Brazilian states, including the zoonotic agent Coxiella burnetii. The used tests were Virus Neutralization Assay for IBR and BVD, Microscopic Agglutination Test for Leptospira spp., Indirect Fluorescent Antibody Test (IFAT) for C. burnetii and Toxoplasma gondii, and Enzyme-Linked Immunosorbent Assay for Neospora caninum and Trypanosoma vivax. Seropositivity for C. burnetii was 13.7% with titers from 128 to 131,072; 57.8% for BoHV-1, with titers between 2 and 1,024; 47.1% for BVDV-1a, with titers from 10 to 5,120; 89.2% for N. caninum; 50% for T. vivax; and 52.0% for Leptospira spp., with titers between 100 to 800 (the following serovars were found: Tarassovi, Grippotyphosa, Canicola, Copenhageni, Wolffi, Hardjo, Pomona and Icterohaemorrhagiae); 19.6% for T. gondii with titer of 40. This is the first study that has identified C. burnetii in cattle associated with BoHV and BVDV, N. caninum, Leptospira spp., T. gondii and T. vivax. Thus, future studies should be conducted to investigate how widespread this pathogen is in Brazilian cattle herds.

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Cross-Sectional Study of Toxoplasma gondii Infection in Pig Farms in England

Foodborne Pathogens and Disease ◽

10.1089/fpd.2016.2197 ◽

2017 ◽

Vol 14 (5) ◽

pp. 269-281 ◽

Cited By ~ 19

Author(s):

Georgina Limon ◽

Wendy Beauvais ◽

Nikolaos Dadios ◽

Isabelle Villena ◽

Charlotte Cockle ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional ◽

Pig Farms ◽

Toxoplasma Gondii Infection

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Occurrence of pathogens in wild rodents caught on Swedish pig and chicken farms

Epidemiology and Infection ◽

10.1017/s0950268812002609 ◽

2012 ◽

Vol 141 (9) ◽

pp. 1885-1891 ◽

Cited By ~ 24

Author(s):

A. BACKHANS ◽

M. JACOBSON ◽

I. HANSSON ◽

M. LEBBAD ◽

S. THISTED LAMBERTZ ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Campylobacter Jejuni ◽

Yersinia Enterocolitica ◽

Encephalomyocarditis Virus ◽

Farm Animals ◽

Wild Rodents ◽

Brachyspira Hyodysenteriae ◽

Pig Farms ◽

Campylobacter Upsaliensis ◽

Selection Of

SUMMARYA total of 207 wild rodents were caught on nine pig farms, five chicken farms and five non-farm locations in Sweden and surveyed for a selection of bacteria, parasites and viruses.Lawsonia intracellulariaand pathogenicYersinia enterocoliticawere only detected in rodents on pig farms (9% and 8% prevalence, respectively) which indicate that these agents are more likely to be transmitted to rodents from pigs or the environment on infected farms.Brachyspira hyodysenteriae(1%),Brachyspira intermedia(2%),Campylobacter jejuni(4%),Campylobacter upsaliensis(2%), leptospires (7%) and encephalomyocarditis virus (9%) were also detected from rodents not in contact with farm animals.GiardiaandCryptosporidiumspp. were common, although no zoonotic types were verified, andSalmonella entericawas isolated from 1/11 mice on one farm but not detected by PCR from any of the rodents.Trichinellaspp. andToxoplasma gondiiwere not detected.

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