Exploring different photosensitizers to optimize elimination of planktonic and biofilm forms of Enterococcus faecalis from infected root canal during antimicrobial photodynamic therapy

2018 ◽  
Vol 24 ◽  
pp. 206-211 ◽  
Author(s):  
Maryam Pourhajibagher ◽  
Hossein Kazemian ◽  
Nasim Chiniforush ◽  
Nava Hosseini ◽  
Babak Pourakbari ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antimicrobial Photodynamic Therapy ◽  
Infected Root ◽  
Infected Root Canal

scholarly journals Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm

2021 ◽  
Author(s):  
Jelena Marinković ◽  
Biljana Nikolić ◽  
Tatjana Marković ◽  
Milena Radunović ◽  
Jugoslav Ilić ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antioxidant Properties ◽  
Thiobarbituric Acid ◽  
Active Principle ◽  
Antibiofilm Activity ◽  
Cymbopogon Citratus ◽  
Infected Root ◽  
Assay Methods ◽  
Infected Root Canal

Aim: The objective was to formulate and characterize the nanoemulsion based on Cymbopogon citratus oil, intended for use in teeth infected root canal therapy. The investigation of the antioxidant and antibiofilm potential toward Enterococcus faecalis was aimed as well. Materials & methods: Characterization of oil (by GC/MS analysis) and nanoemulsion (by dynamic light scattering instrument), and determination of antibacterial (by microdilution assay), antibiofilm (by crystal violet assay) and antioxidant properties (by 2,2-diphenyl-1-picryl-hydrazyl-hydrate and thiobarbituric acid assay methods) were provided. Antibiofilm efficacy of irrigation procedure including nanoemulsion was screened on extracted teeth (by CFU counting assay). Results: Notable antibacterial and antibiofilm activity, both against forming and preformed biofilms of oil, was observed. Irrigation involved nanoemulsion showed remarkable antibiofilm potential. Both substances induced some antioxidant activity. Conclusion: Results encourage further research with the aim of application of the nanoemulsion in dental practice.

scholarly journals Comparison of Antibacterial Effects of Photodynamic Therapy and an Irrigation Activation System on Root Canals Infected With Enterococcus faecalis: An In Vitro Study

2020 ◽  
Vol 11 (3) ◽  
pp. 243-248
Author(s):  
Mohammad Asnaashari ◽  
Niloufar Kooshki ◽  
Mohammad Mahdi Salehi ◽  
Saranaz Azari-Marhabi ◽  
Hedieh Amin Moghadassi
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
New Technologies ◽  
Irrigation System ◽  
Bacterial Colony ◽  
Root Canals ◽  
Infected Root ◽  
Activation System

Introduction: Enterococcus faecalis is a resistant bacterium which is the most abundant species in infected root canals. Photodynamic therapy (PDT) is a method for killing the bacteria with active Oxygen radicals generated in a photosensitizer when exposed to centralized light. Furthermore, as a new method of canal disinfection, a variety of irrigation activation systems have been introduced, one of which is GentleFile (GF) with rotary movements and spiral effects for antibacterial action. This study aimed to compare the effectiveness of the two mentioned methods when used with and without Sodium Hypochlorite in eliminating E. faecalis from infected root canals. Methods: Fifty-eight uniradicular teeth were randomly divided into 4 experimental groups of 14. Two specimens were selected for later scanning electron microscopy in order to screen the procedure steps. In each experimental group, 10 samples were selected to be treated with GF or PDT; 3 of them were selected as positive controls and the other one sample was chosen as a negative control. Experimental groups were as follows: (1) Irrigation activation system, (2) Irrigation activation system + sodium hypochlorite, (3) PDT, and (4) PDT+ sodium hypochlorite. The specimens were then cultured for a bacterial colony count. Results: The decrease in the bacterial count after the treatment with the irrigation activation system was 99.8% (P=0.011) and when the system was used with sodium hypochlorite, it was 100% (P=0.001). The antibacterial effect of PDT was 90.08% (P=0.011) and it was 99.7% when PDT was combined with sodium hypochlorite (P=0.011). Conclusion: All four methods can be administered as complementary methods in root canal disinfection. According to the results of disinfection in the experimental groups of current study it is concluded that integration of new technologies such as activation irrigation system or PDT in Combination with NaOCl ameliorates disinfection of root canal and can provide several advantages in the endodontic outcome.

The Effect of Pluchea indica Less Leaves Extract Againts Biofilm of Enterococcus faecalis and Fusobacterium nucleatum In Vitro

DENTA ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 51
Author(s):  
Agni Febrina Pargaputri ◽  
Elly Munadziroh ◽  
Retno Indrawati
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Biofilm Formation ◽  
Chemical Properties ◽  
Fusobacterium Nucleatum ◽  
Bacterial Biofilm ◽  
Dilution Method ◽  
Infected Root ◽  
Infected Root Canal

<p><strong><em>Background:</em></strong><em> Enterococcus faecalis and Fusobacterium nucleatum are the most common bacteria found in infected root canal teeth and most of them often caused failure in endodontic treatments. These bacteria can form biofilm which makes them more resistant against antibacterial agents. Biofilm formation also causes a decrease in antibiotics and antimicrobials sensitivity. Pluchea indica Less leaves is a species of plants that has several chemical properties. It consists of flavonoids and polyphenols which have benefits to inhibit biofilm formation. Because of its benefits, the extract of Pluchea indica Less leaves can be potentially developed as one of  sterilization dressing in root canal teeth. <strong>Purpose: </strong>The aim of this study was to determine biofilm formation inhibition of Pluchea indica Less leaves extract against Enterococcus faecalis and Fusobacterium nucleatum. <strong>Materials and Methods: </strong>The dilution method was done first to show the Minimum Inhibitory Concentration (MIC) of the extract. The inhibition biolfilm formation was tested using microtitter plate assay by measuring the bacterial biofilm Optical Density (OD) from ELISA reader’s results and using autoagregation assay to show the inhibition of adherance bacteria.  The Pluchea indica Less leaves extract concentration used for inhibition of biofilm formation were 100%, 50%, 25%, 12,5%, and 6,25%. <strong>Results:</strong> The result of biofilm formation inhibition showed that Pluchea indica Less leaves extract were able to inhibit Enterococcus faecalis and Fusobacterium nucleatum’ biofilm formation with strong moderate effect. The autoagregation assay showed a decrease in autoagregation percentation of Enterococcus faecalis and Fusobacterium nucleatum. <strong>Conclusions:</strong> Pluchea indica Less leaves extract has effect to inhibit biofilm formation of Enterococcus faecalis and Fusobacterium nucleatum.</em></p><p><strong><em>Keywords: </em></strong><em>Pluchea indica Less leaves extract, Enterococcus faecalis, Fusobacterium nucleatum, biofilm.</em></p><p><strong><em>Correspondence:</em></strong><em> Agni Febrina Pargaputri, Department of Oral Biology, </em><em>Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone.031-5912191</em><em>, Email: <span style="text-decoration: underline;">[email protected]</span></em><strong><em></em></strong></p>

Pemilihan medikamen intrakanal antar kunjungan yang rasional

2010 ◽  
Vol 9 (1) ◽  
pp. 63
Author(s):  
Indrya Kirana Mattulada
Keyword(s):  
Root Canal ◽  
Endodontic Treatment ◽  
Infected Root ◽  
Infected Root Canal

Bacteria and their products play a fundamental role in the initiation, propagation andpersistence of pulpal and periradicular diseases. Consequently one of the major goalsof endodontic treament is to eliminate all bacteria from the root canal space.Elimination of microorganism from infected root canal is a complicated task.Numerous measures have been described to reduce the numbers of root canalmicroorganism, including the use of various instrumentation techniques, irrigationregimens, and intracanal medication. In endodontic treatment, there are severalmedicament used traditionally. They are phenolic, aldehyde, halogen, calciumhydroxide, and antibiotic. The aim of this paper is to discuss about indication,contraindication and effect of each medicament.

scholarly journals The Effect of Antimicrobial Photodynamic Therapy Using Chlorophyllin–Phycocyanin Mixture on Enterococcus faecalis: The Influence of Different Light Sources

2020 ◽  
Vol 10 (12) ◽  
pp. 4290 ◽  
Author(s):  
Nasim Chiniforush ◽  
Maryam Pourhajibagher ◽  
Steven Parker ◽  
Stefano Benedicenti ◽  
Abbas Bahador ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Diode Laser ◽  
Enterococcus Faecalis ◽  
Control Group ◽  
Human Gingival Fibroblast ◽  
Light Sources ◽  
Gingival Fibroblast ◽  
Antimicrobial Photodynamic Therapy ◽  
Cell Viability Assay ◽  
Vitro Effect

The purpose of this study was to evaluate the in vitro effect of the chlorophyllin–phycocyanin mixture (Photoactive+) as a photosensitizer (PS) during antimicrobial photodynamic therapy (aPDT) on the count of Enterococcus faecalis (E. faecalis) using different light sources. The antimicrobial effect of aPDT with chlorophyllin–phycocyanin mixture using different light sources including diode laser (λ = 660 nm), diode laser (λ = 635 nm), LED (λ = 450 ± 30 nm) alone or in combination was assessed using microbial cell viability assay against E. faecalis. In addition, the cell cytotoxicity of Photoactive+ was assessed on human gingival fibroblast (HuGu) cells by MTT assay; E. faecalis growth when treated by both red wavelengths (635 nm, 660 nm) and combination of LED (420–480 nm) and red wavelengths (635 nm, 660 nm), significantly reduced compared to the control group (p < 0.05). There was no significant reduction in the number of viable cells exposed to Photoactive+ compared to the control group (p < 0.05). This study shows that the application of chlorophyllin–phycocyanin mixture and irradiation with emission of red light achieved a better result for bacterial count reduction, compared to a control. This component can be applied safely due to very negligible cytotoxicity.

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