Effect of red and blue light on the timing of cyclin-dependent kinase activity and the timing of cell division in Chlamydomonas reinhardtii

2004 ◽  
Vol 42 (4) ◽  
pp. 341-348 ◽  
Author(s):  
Harriëtte Oldenhof ◽  
Kateřina Bišová ◽  
Herman van den Ende ◽  
Vilém Zachleder
Keyword(s):  
Cell Division ◽  
Chlamydomonas Reinhardtii ◽  
Blue Light ◽  
Kinase Activity ◽  
Cyclin Dependent Kinase

The alga Chlamydomonas reinhardtii UVS11 gene is responsible for cell division delay and temporal decrease in histone H1 kinase activity caused by UV irradiation

DNA Repair ◽  
2003 ◽  
Vol 2 (6) ◽  
pp. 737-750 ◽  
Author(s):  
Miroslava Slaninová ◽  
Barbara Nagyová ◽  
Eliška Gálová ◽  
Jana Hendrychová ◽  
Kateřina Bišová ◽  
...  
Keyword(s):  
Cell Division ◽  
Chlamydomonas Reinhardtii ◽  
Uv Irradiation ◽  
Kinase Activity ◽  
Histone H1

scholarly journals A CDK-related kinase regulates the length and assembly of flagella in Chlamydomonas

2007 ◽  
Vol 176 (6) ◽  
pp. 819-829 ◽  
Author(s):  
Lai-Wa Tam ◽  
Nedra F. Wilson ◽  
Paul A. Lefebvre
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Kinase Activity ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Binding Motif ◽  
Cell Fractionation ◽  
Cyclin Dependent Kinase ◽  
Yeast Two Hybrid ◽  
Flagellar Assembly ◽  
Two Hybrid

Little is known about how cells regulate the size of their organelles. In this study, we find that proper flagellar length control in Chlamydomonas reinhardtii requires the activity of a new member of the cyclin-dependent kinase (CDK) family, which is encoded by the LF2 (long flagella 2) gene. This novel CDK contains all of the important residues that are essential for kinase activity but lacks the cyclin-binding motif PSTAIRE. Analysis of genetic lesions in a series of lf2 mutant alleles and site-directed mutagenesis of LF2p reveals that improper flagellar length and defective flagellar assembly correlate with the extent of disruption of conserved kinase structures or residues by mutations. LF2p appears to interact with both LF1p and LF3p in the cytoplasm, as indicated by immunofluorescence localization, sucrose density gradients, cell fractionation, and yeast two-hybrid experiments. We propose that LF2p is the catalytic subunit of a regulatory kinase complex that controls flagellar length and flagellar assembly.

scholarly journals Variation in Growth Rate between Arabidopsis Ecotypes Is Correlated with Cell Division and A-Type Cyclin-Dependent Kinase Activity

2002 ◽  
Vol 129 (2) ◽  
pp. 854-864 ◽  
Author(s):  
Gerrit T.S. Beemster ◽  
Kristof De Vusser ◽  
Evelien De Tavernier ◽  
Kirsten De Bock ◽  
Dirk Inzé
Keyword(s):  
Growth Rate ◽  
Cell Division ◽  
Kinase Activity ◽  
Cyclin Dependent Kinase

Blue Light Delays Commitment to Cell Division in Chlamydomonas reinhardtii

Plant Biology ◽  
2004 ◽  
Vol 6 (6) ◽  
pp. 689-695 ◽  
Author(s):  
H. Oldenhof ◽  
V. Zachleder ◽  
H. Ende
Keyword(s):  
Cell Division ◽  
Chlamydomonas Reinhardtii ◽  
Blue Light ◽  
Commitment To Cell Division

scholarly journals Response of the Green Alga Chlamydomonas reinhardtii to the DNA Damaging Agent Zeocin

Cells ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 735 ◽  
Author(s):  
Mária Čížková ◽  
Monika Slavková ◽  
Milada Vítová ◽  
Vilém Zachleder ◽  
Kateřina Bišová
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Chlamydomonas Reinhardtii ◽  
Green Alga ◽  
Cell Cycle Progression ◽  
Kinase Activity ◽  
Cyclin Dependent Kinase ◽  
Cycle Progression ◽  
Mitotic Cyclin ◽  
Cell Cycle Block

DNA damage is a ubiquitous threat endangering DNA integrity in all living organisms. Responses to DNA damage include, among others, induction of DNA repair and blocking of cell cycle progression in order to prevent transmission of damaged DNA to daughter cells. Here, we tested the effect of the antibiotic zeocin, inducing double stranded DNA breaks, on the cell cycle of synchronized cultures of the green alga Chlamydomonas reinhardtii. After zeocin application, DNA replication partially occurred but nuclear and cellular divisions were completely blocked. Application of zeocin combined with caffeine, known to alleviate DNA checkpoints, decreased cell viability significantly. This was probably caused by a partial overcoming of the cell cycle progression block in such cells, leading to aberrant cell divisions. The cell cycle block was accompanied by high steady state levels of mitotic cyclin-dependent kinase activity. The data indicate that DNA damage response in C. reinhardtii is connected to the cell cycle block, accompanied by increased and stabilized mitotic cyclin-dependent kinase activity.

scholarly journals Blue Light Regulation of Cell Division in Chlamydomonas reinhardtii

1992 ◽  
Vol 99 (4) ◽  
pp. 1370-1375 ◽  
Author(s):  
Petra Münzner ◽  
Jürgen Voigt
Keyword(s):  
Cell Division ◽  
Chlamydomonas Reinhardtii ◽  
Blue Light ◽  
Light Regulation

Activity and expression pattern of cyclin-dependent kinase 5 in the embryonic mouse nervous system

Development ◽  
1993 ◽  
Vol 119 (4) ◽  
pp. 1029-1040 ◽  
Author(s):  
L.H. Tsai ◽  
T. Takahashi ◽  
V.S. Caviness ◽  
E. Harlow
Keyword(s):  
Nervous System ◽  
Cell Division ◽  
Cell Division Cycle ◽  
Adult Brain ◽  
Cyclin Dependent Kinase ◽  
Proliferating Cells ◽  
Embryonic Mouse ◽  
Postmitotic Neurons ◽  
Cyclin Dependent Kinase 5 ◽  
Expression And Activity

Cyclin-dependent kinase 5 (cdk5) was originally isolated on the basis of its close primary sequence homology to the human cdc2 serine/threonine kinase, the prototype of the cyclin-dependent kinases. While kinase activities of both cdc2 and cdk2 are detected in proliferating cells and are essential for cells to progress through the key transition points of the cell cycle, cdk5 kinase activity has been observed only in lysates of adult brain. In this study, we compared the activity and expression of cdk5 with that of cdc2 and cdk2 in the embryonic mouse forebrain. The expression and activity of cdk5 increased progressively as increasing numbers of cells exited the proliferative cycle. In contrast, the expression and activity of cdc2 and cdk2 were maximum at gestational day 11 (E11) when the majority of cells were proliferating and fell to barely detectable levels at E17 at the end of the cytogenetic period. Immunohistochemical studies showed that cdk5 is expressed in postmitotic neurons but not in glial cells or mitotically active cells. Expression of cdk5 was concentrated in fasciculated axons of postmitotic neurons. In contrast to other cell division cycle kinases to which it is closely related, cdk5 appears not to be expressed in dividing cells in the developing brain. These observations suggest that cdk5 may have a role in neuronal differentiation but not in the cell division cycle in the embryonic nervous system.

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