Insight into VvGH3 genes evolutional relationship from monocotyledons and dicotyledons reveals that VvGH3-9 negatively regulates the drought tolerance in transgenic Arabidopsis

2022 ◽  
Author(s):  
Shixiong Lu ◽  
Ping Wang ◽  
Guojie Nai ◽  
Yanmei Li ◽  
Yanli Su ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Insight Into

scholarly journals TaNAC29, a NAC transcription factor from wheat, enhances salt and drought tolerance in transgenic Arabidopsis

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Quanjun Huang ◽  
Yan Wang ◽  
Bin Li ◽  
Junli Chang ◽  
Mingjie Chen ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Nac Transcription Factor ◽  
Salt And Drought Tolerance

Overexpression of a maize MYB48 gene confers drought tolerance in transgenic arabidopsis plants

2017 ◽  
Vol 60 (6) ◽  
pp. 612-621 ◽  
Author(s):  
Yan Wang ◽  
Qianqian Wang ◽  
MingLi Liu ◽  
Chen Bo ◽  
Xi Wang ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Transgenic Arabidopsis Plants

A wheat R2R3 MYB gene TaMpc1-D4 negatively regulates drought tolerance in transgenic Arabidopsis and wheat

Plant Science ◽  
2020 ◽  
Vol 299 ◽  
pp. 110613 ◽  
Author(s):  
Xiaorui Li ◽  
Yan Tang ◽  
Hailan Li ◽  
Wen Luo ◽  
Chunju Zhou ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Myb Gene ◽  
R2r3 Myb

scholarly journals The Maize Clade A PP2C Phosphatases Play Critical Roles in Multiple Abiotic Stress Responses

2019 ◽  
Vol 20 (14) ◽  
pp. 3573 ◽  
Author(s):  
Zhenghua He ◽  
Jinfeng Wu ◽  
Xiaopeng Sun ◽  
Mingqiu Dai
Keyword(s):  
Drought Tolerance ◽  
Stress Responses ◽  
Transgenic Arabidopsis ◽  
Expression Patterns ◽  
Survival Rates ◽  
Wild Type ◽  
Multiple Stresses ◽  
Core Components ◽  
Leaf Water Loss ◽  
Natural Variations

As the core components of abscisic acid (ABA) signal pathway, Clade A PP2C (PP2C-A) phosphatases in ABA-dependent stress responses have been well studied in Arabidopsis. However, the roles and natural variations of maize PP2C-A in stress responses remain largely unknown. In this study, we investigated the expression patterns of ZmPP2C-As treated with multiple stresses and generated transgenic Arabidopsis plants overexpressing most of the ZmPP2C-A genes. The results showed that the expression of most ZmPP2C-As were dramatically induced by multiple stresses (drought, salt, and ABA), indicating that these genes may have important roles in response to these stresses. Compared with wild-type plants, ZmPP2C-A1, ZmPP2C-A2, and ZmPP2C-A6 overexpression plants had higher germination rates after ABA and NaCl treatments. ZmPP2C-A2 and ZmPP2C-A6 negatively regulated drought responses as the plants overexpressing these genes had lower survival rates, higher leaf water loss rates, and lower proline accumulation compared to wild type plants. The natural variations of ZmPP2C-As associated with drought tolerance were also analyzed and favorable alleles were detected. We widely studied the roles of ZmPP2C-A genes in stress responses and the natural variations detected in these genes have the potential to be used as molecular markers in genetic improvement of maize drought tolerance.

scholarly journals CsCYT75B1, a Citrus CYTOCHROME P450 Gene, Is Involved in Accumulation of Antioxidant Flavonoids and Induces Drought Tolerance in Transgenic Arabidopsis

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 161 ◽  
Author(s):  
Muhammad Junaid Rao ◽  
Yuantao Xu ◽  
Xiaomei Tang ◽  
Yue Huang ◽  
Jihong Liu ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Wild Type ◽  
Ros Scavenging ◽  
Cytochrome P450 Gene ◽  
P450 Gene ◽  
Large Gene ◽  
Transgenic Lines

CYTOCHROME P450s genes are a large gene family in the plant kingdom. Our earlier transcriptome data revealed that a CYTOCHROME P450 gene of Citrus sinensis (CsCYT75B1) was associated with flavonoid metabolism and was highly induced after drought stress. Here, we characterized the function of CsCYT75B1 in drought tolerance by overexpressing it in Arabidopsis thaliana. Our results demonstrated that the overexpression of the CsCYT75B1 gene significantly enhanced the total flavonoid contents with increased antioxidant activity in transgenic Arabidopsis. The gene expression results showed that several genes that are responsible for the biosynthesis of antioxidant flavonoids were induced by 2–12 fold in transgenic Arabidopsis lines. After 14 days of drought stress, all transgenic lines displayed an enhanced tolerance to drought stress along with accumulating antioxidant flavonoids with lower superoxide radicals and reactive oxygen species (ROS) than wild type plants. In addition, drought-stressed transgenic lines possessed higher antioxidant enzymatic activities than wild type transgenic lines. Moreover, the stressed transgenic lines had significantly lower levels of electrolytic leakage than wild type transgenic lines. These results demonstrate that the CsCYT75B1 gene of sweet orange functions in the metabolism of antioxidant flavonoid and contributes to drought tolerance by elevating ROS scavenging activities.

scholarly journals Maize ZmBES1/BZR1-5 Decreases ABA Sensitivity and Confers Tolerance to Osmotic Stress in Transgenic Arabidopsis

2020 ◽  
Vol 21 (3) ◽  
pp. 996 ◽  
Author(s):  
Fuai Sun ◽  
Haoqiang Yu ◽  
Jingtao Qu ◽  
Yang Cao ◽  
Lei Ding ◽  
...  
Keyword(s):  
Stress Response ◽  
Drought Tolerance ◽  
Osmotic Stress ◽  
Transgenic Arabidopsis ◽  
Salt And Drought Tolerance ◽  
Aba Sensitivity ◽  
E Box ◽  
Mda Content ◽  
Response To Stress ◽  
Stress Related Genes

The BRI1-EMS suppressor 1 (BES1)/brassinazole-resistant 1 (BZR1) transcription factors, key components in the brassinosteroid signaling pathway, play pivotal roles in plant growth and development. However, the function of BES1/BZR1 in crops during stress response remains poorly understood. In the present study, we characterized ZmBES1/BZR1-5 from maize, which was localized to the nucleus and was responsive to abscisic acid (ABA), salt and drought stresses. Heterologous expression of ZmBES1/BZR1-5 in transgenic Arabidopsis resulted in decreased ABA sensitivity, facilitated shoot growth and root development, and enhanced salt and drought tolerance with lower malondialdehyde (MDA) content and relative electrolyte leakage (REL) under osmotic stress. The RNA sequencing (RNA-seq) analysis revealed that 84 common differentially expressed genes (DEGs) were regulated by ZmBES1/BZR1-5 in transgenic Arabidopsis. Subsequently, gene ontology and KEGG pathway enrichment analyses showed that the DEGs were enriched in response to stress, secondary metabolism and metabolic pathways. Furthermore, 30 DEGs were assigned to stress response and possessed 2–15 E-box elements in their promoters, which could be potentially recognized and bound by ZmBES1/BZR1-5. Taken together, our results reveal that the ZmBES1/BZR1-5 transcription factor positively regulates salt and drought tolerance by binding to E-box to induce the expression of downstream stress-related genes. Therefore, our study contributes to the better understanding of BES1/BZR1 function in the stress response of plants.

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