Erratum to “Modelling a risk classification of aneuploidy in human embryos using non-invasive morphokinetics” [Reprod. BioMed. Online 26 (2013) 477–485]

Abstract Human embryos utilise an array of processes to eliminate the very high prevalence of aneuploid cells in early embryo stages. Human embryo self-correction was recently demonstrated by their ability to eliminate/expel abnormal blastomeres as cell debris/fragments. A whole genome amplification study has demonstrated that 63.6% of blastocysts expelled cell debris with abnormal chromosomal rearrangements. Moreover, 55.5% of euploid blastocysts expel aneuploid debris, strongly suggesting that the primary source of cell free DNA in culture media is expelled aneuploid blastomeres and/or their fragments. Such a substantial ability to self-correct downstream from the blastocyststage, therefore, renders any chromosomal diagnosis at the blastocyststage potentially useless, and this, unfortunately, also must particularly include non-invasive PGT-A based on cell-free DNA in spent medium. High rates of false-positive diagnoses of human embryos often lead to non-use and/or disposal of embryos with entirely normal pregnancy potential. Before adopting yet another round of unvalidated PGT-A as a routine adjunct to IVF, we here present facts that deserve to be considered.

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Improving intermediate risk classification of prostate cancer with radiomics features

European Urology ◽

10.1016/s0302-2838(21)01304-x ◽

2021 ◽

Vol 79 ◽

pp. S1309

Author(s):

R. Thenault ◽

L. Beuzit ◽

N. Rioux-Leclercq ◽

Z-E. Khene ◽

A. Gasmi ◽

...

Keyword(s):

Prostate Cancer ◽

Risk Classification ◽

Intermediate Risk

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Efficacy of estramustine phosphate according to risk classification of castration-resistant prostate cancer

Medical Oncology ◽

10.1007/s12032-012-0178-z ◽

2012 ◽

Vol 29 (4) ◽

pp. 2895-2900 ◽

Cited By ~ 8

Author(s):

Akinori Minato ◽

Naohiro Fujimoto ◽

Tatsuhiko Kubo ◽

Shuji Harada ◽

Soichiro Akasaka ◽

...

Keyword(s):

Prostate Cancer ◽

Risk Classification ◽

Estramustine Phosphate ◽

Castration Resistant Prostate Cancer ◽

Castration Resistant

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A57 THE USE AND INTERPRETATION OF HLA-DQ2/DQ8 GENOTYPING BY PEDIATRIC GASTROENTEROLOGISTS

Journal of the Canadian Association of Gastroenterology ◽

10.1093/jcag/gwab002.055 ◽

2021 ◽

Vol 4 (Supplement_1) ◽

pp. 14-15

Author(s):

B Moreau ◽

E Robidoux

Keyword(s):

Risk Groups ◽

Risk Classification ◽

Low Risk ◽

Risk Alleles ◽

Seronegative Patient ◽

Espghan Guidelines ◽

Recent Classification ◽

American Society ◽

Hla Dq2

Abstract Background A recent classification of high and low risk alleles associated with celiac disease (CD) shows that the presence of a single allele (DQA1*05 or DQB1*02; coding together for HLA-DQ2), without a positive genotype (HLA-DQ2 and or HLA-DQ8), represents a risk of developing the disease. Aims The aim of this study is to evaluate the use and interpretation of the HLA-DQ2/DQ8 genotyping by pediatric gastroenterologists, as there is no study on the matter and the latest guidelines do not address this risk classification. Methods A web-based survey was sent by email to all NASPGHAN (North American society of pediatric gastroenterolgy, hepatology and nutrition) members. Results Results 294 pediatric gastroenterologists sent a complete survey. 86,1% use the HLA-DQ2/DQ8 genotyping according mainly to the NASPGHAN and ESPGHAN guidelines. The main indications considered were to exclude CD in a patient on a gluten-free diet with a resolution of his symptoms and in a seronegative patient with equivocal biopsies. A minority would consider the genotyping for screening high risk groups or for making a diagnosis in children with high specific CD antibodies and strong clinical suspicion without performing biopsies, as suggested by the ESPGHAN guidelines. The alleles associated with CD are not well known, but 76,7% the participants are aware of the risk classification. While only 62,8% have access to the complete genotype, 47,8% consider it useful. Nevertheless, 82,6% would still want to know the presence of a low risk allele. Conclusions The risk classification of alleles related to CD warrants a modification of the genotyping result with access to the alleles and an adaptation of the guidelines. Funding Agencies None

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P–331 The #Enzian classification: A comprehensive non-invasive and surgical description system for endometriosis

Human Reproduction ◽

10.1093/humrep/deab130.330 ◽

2021 ◽

Vol 36 (Supplement_1) ◽

Author(s):

J Keckstein ◽

H Gernot

Keyword(s):

Classification System ◽

Working Group ◽

Complex Disease ◽

Diagnostic Methods ◽

Anatomical Location ◽

Ovarian Endometriosis ◽

Deep Endometriosis ◽

Non Invasive ◽

Complete Mapping

Abstract Study question Is there a classification for a complete mapping of endometriosis, including anatomical location, size of the lesions, and degree of involvement that can be used with both, diagnostics and surgery? Summary answer #Enzian classification improves in both, non-invasive diagnostic methods and surgical therapy for endometriosis as a universally usable classification system for all aspects of the disease. What is known already The most commonly used r-ASRM classification has certain limitations due to its incomplete description of DE, the complexity of the classification, and lack of reproducibility. In contrast, the Enzian classification, which has been implemented in the last decade, has proved to be the most suitable for the description of DE. However, since it does not include peritoneal and ovarian lesions and lacks a description of tubo-ovarian adhesions, it has not gained full acceptance. A combination of classification with different systems such as r-ASRM, EFI score and Enzian, may complicate classification of the disease due to overlaps and time-consuming documentation. Study design, size, duration The result is a consensus of a panel of renowned clinicians (working group), gynaecological surgeons and sonographers with extensive expertise in diagnosis and therapy of endometriosis. A first draft was written in 2019 by a joint effort of the first and last author and sent to all working group members. Taking all comments into account, a revised draft was then sent to all coauthors and repeated until a consensus was reached (9 revisions). Participants/materials, setting, methods Criteria used to invite the experts to participate in this consensus process included their having significant peer-reviewed publications in the field of diagnosis and management of endometriosis. Main results and the role of chance Our current proposal is the first of its kind to universally describe superficial and deep endometriosis, ovarian endometriosis, adenomyosis and adhesions by using a classification system that can be applied by gynaecologists, surgeons, sonographers and radiologists following the same principles. The correlation between preoperative and surgical staging, on the basis of the Enzian scheme, allows for consistent and clear classification of endometriosis, especially DE. Endometriosis can be mapped completely with one single classification system enabling the use of one common language. Limitations, reasons for caution This classification system is anatomically logical and should be easy to use. Further studies are ongoing and are needed to provide proof for the applicability, reproducibility and accuracy of the #Enzian classification for the description of endometriosis. Wider implications of the findings: #Enzian classification now enabled better coverage of various endometriosis localizations. The possibility of using this system preoperatively as well as postoperatively within the framework of diagnostics offers clinicians a significant improvement in the care of patients with such a complex disease. Trial registration number Not applicable

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Non-invasive pre-implantation genetic testing of human embryos: an emerging concept

Human Reproduction ◽

10.1093/humrep/dey314 ◽

2018 ◽

Vol 33 (12) ◽

pp. 2162-2167 ◽

Cited By ~ 8

Author(s):

C Farra ◽

F Choucair ◽

J Awwad

Keyword(s):

Genetic Testing ◽

Human Embryos ◽

Non Invasive

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Pain in emergency units: correlation with risk classification categories

Revista Latino-Americana de Enfermagem ◽

10.1590/1518-8345.2415.3070 ◽

2018 ◽

Vol 26 (0) ◽

Cited By ~ 1

Author(s):

Wandressa Letícia Viveiros ◽

Meiry Fernanda Pinto Okuno ◽

Cássia Regina Vancini Campanharo ◽

Maria Carolina Barbosa Teixeira Lopes ◽

Gabriella Novelli Oliveira ◽

...

Keyword(s):

Signs And Symptoms ◽

Medical Specialty ◽

Cross Sectional Study ◽

Risk Classification ◽

Moderate Intensity ◽

Ratio Test ◽

Intense Pain ◽

Cross Sectional ◽

Mild Pain

ABSTRACT Objectives: to correlate risk classification categories with the level of pain of patients in an emergency service. Method: cross-sectional study carried out in the Risk Classification of 611 patients. The variables studied were: age, gender, comorbidities, complaint duration, medical specialty, signs and symptoms, outcome, color attributed in the risk classification of and degree of pain. We used Analysis of Variance, a Chi-Square test and a Likelihood Ratio test. Results: the average age was 42.1 years (17.8); 59.9% were women; the green (58.9%) and yellow (22.7%) risk classification prevailed and hypertension (18.3%) was the most common Comorbidity. The most frequent pain intensity was moderate (25.9%). In the red category, patients presented a higher percentage of absence of pain; in the blue, mild pain; and in the green, yellow and orange categories, there was a greater percentage of intense pain (p < 0.0001). Conclusion: among the patients who presented pain, the majority reported moderate intensity. Regarding risk categories, most patients in the red category did not report pain. Those who were classified as green, yellow and orange, reported mostly intense pain. On the other hand, patients in the blue category reported predominantly mild pain.

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Portal hypertension and its management in children

Archives of Disease in Childhood ◽

10.1136/archdischild-2015-310022 ◽

2017 ◽

Vol 103 (2) ◽

pp. 186-191 ◽

Cited By ~ 9

Author(s):

Tassos Grammatikopoulos ◽

Patrick James McKiernan ◽

Anil Dhawan

Keyword(s):

Portal Hypertension ◽

Liver Disease ◽

Gastrointestinal Bleeding ◽

Surgical Procedures ◽

Vascular Occlusion ◽

Portal System ◽

Mortality And Morbidity ◽

Non Invasive ◽

Intravascular Pressure

Portal hypertension (PHT), defined as raised intravascular pressure in the portal system, is a complication of chronic liver disease or liver vascular occlusion. Advances in our ability to diagnose and monitor the condition but also predict the risk of gastrointestinal bleeding have enabled us to optimise the management of children with PHT either at a surveillance or at a postbleeding stage. A consensus among paediatric centres in the classification of varices can be beneficial in streamlining future paediatric studies. New invasive (endoscopic and surgical procedures) and non-invasive (pharmacotherapy) techniques are currently used enabling clinicians to reduce mortality and morbidity in children with PHT.

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