Serotyping and antimicrobial resistance of Mannheimia haemolytica strains from European cattle with bovine respiratory disease

A broad, cross-sectional study of beef cattle at entry into Canadian feedlots investigated the prevalence and epidemiology of antimicrobial resistance (AMR) in Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, and Mycoplasma bovis, bacterial members of the bovine respiratory disease (BRD) complex. Upon feedlot arrival and before antimicrobials were administered at the feedlot, deep nasopharyngeal swabs were collected from 2,824 feedlot cattle in southern and central Alberta, Canada. Data on the date of feedlot arrival, cattle type (beef, dairy), sex (heifer, bull, steer), weight (kg), age class (calf, yearling), source (ranch direct, auction barn, backgrounding operations), risk of developing BRD (high, low), and weather conditions at arrival (temperature, precipitation, and estimated wind speed) were obtained. Mannheimia haemolytica, P. multocida, and H. somni isolates with multidrug-resistant (MDR) profiles associated with the presence of integrative and conjugative elements were isolated more often from dairy-type than from beef-type cattle. Our results showed that beef-type cattle from backgrounding operations presented higher odds of AMR bacteria as compared to auction-derived calves. Oxytetracycline resistance was the most frequently observed resistance across all Pasteurellaceae species and cattle types. Mycoplasma bovis exhibited high macrolide minimum inhibitory concentrations in both cattle types. Whether these MDR isolates establish and persist within the feedlot environment, requires further evaluation.

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Comparative efficacy of enrofloxacin to that of tulathromycin for the control of bovine respiratory disease and prevalence of antimicrobial resistance in Mannheimia haemolytica in calves at high risk of developing bovine respiratory disease1

Journal of Animal Science ◽

10.1093/jas/sky054 ◽

2018 ◽

Vol 96 (4) ◽

pp. 1259-1267 ◽

Cited By ~ 12

Author(s):

S Crosby ◽

B Credille ◽

S Giguère ◽

R Berghaus

Keyword(s):

High Risk ◽

Antimicrobial Resistance ◽

Respiratory Disease ◽

Bovine Respiratory Disease ◽

Mannheimia Haemolytica ◽

Comparative Efficacy

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Comparison of Mannheimia haemolytica isolates from an outbreak of bovine respiratory disease

Veterinary Microbiology ◽

10.1016/j.vetmic.2015.10.020 ◽

2016 ◽

Vol 182 ◽

pp. 82-86 ◽

Cited By ~ 3

Author(s):

S. Rainbolt ◽

D.K. Pillai ◽

B.V. Lubbers ◽

M. Moore ◽

R. Davis ◽

...

Keyword(s):

Respiratory Disease ◽

Bovine Respiratory Disease ◽

Mannheimia Haemolytica

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Cross protection of a Mannheimia haemolytica A1 Lkt-/Pasteurella multocida ΔhyaE bovine respiratory disease vaccine against experimental challenge with Mannheimia haemolytica A6 in calves

Vaccine ◽

10.1016/j.vaccine.2012.01.063 ◽

2012 ◽

Vol 30 (13) ◽

pp. 2320-2328 ◽

Cited By ~ 10

Author(s):

C.F. Crouch ◽

R. LaFleur ◽

C. Ramage ◽

D. Reddick ◽

J. Murray ◽

...

Keyword(s):

Respiratory Disease ◽

Pasteurella Multocida ◽

Bovine Respiratory Disease ◽

Cross Protection ◽

Mannheimia Haemolytica ◽

Experimental Challenge

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Development of Bacterial Therapeutics against the Bovine Respiratory Pathogen Mannheimia haemolytica

Applied and Environmental Microbiology ◽

10.1128/aem.01359-19 ◽

2019 ◽

Vol 85 (21) ◽

Cited By ~ 6

Author(s):

Samat Amat ◽

Edouard Timsit ◽

Danica Baines ◽

Jay Yanke ◽

Trevor W. Alexander

Keyword(s):

Respiratory Disease ◽

Animal Health ◽

Bovine Respiratory Disease ◽

Feedlot Cattle ◽

Mitigation Strategies ◽

Commensal Bacteria ◽

Mannheimia Haemolytica ◽

Respiratory Pathogen ◽

Content Type

ABSTRACT Bovine respiratory disease (BRD) is a major cause of morbidity and mortality in beef cattle. Recent evidence suggests that commensal bacteria of the bovine nasopharynx have an important role in maintaining respiratory health by providing colonization resistance against pathogens. The objective of this study was to screen and select bacterial therapeutic candidates from the nasopharynxes of feedlot cattle to mitigate the BRD pathogen Mannheimia haemolytica. In a stepwise approach, bacteria (n = 300) isolated from the nasopharynxes of 100 healthy feedlot cattle were identified and initially screened (n = 178 isolates from 12 different genera) for growth inhibition of M. haemolytica. Subsequently, selected isolates were evaluated for the ability to adhere to bovine turbinate (BT) cells (n = 47), compete against M. haemolytica for BT cell adherence (n = 15), and modulate gene expression in BT cells (n = 10). Lactobacillus strains had the strongest inhibition of M. haemolytica, with 88% of the isolates (n =33) having inhibition zones ranging from 17 to 23 mm. Adherence to BT cells ranged from 3.4 to 8.0 log10 CFU per 105 BT cells. All the isolates tested in competition assays reduced M. haemolytica adherence to BT cells (32% to 78%). Among 84 bovine genes evaluated, selected isolates upregulated expression of interleukin 8 (IL-8) and IL-6 (P < 0.05). After ranking isolates for greatest inhibition, adhesion, competition, and immunomodulation properties, 6 Lactobacillus strains from 4 different species were selected as the best candidates for further development as intranasal bacterial therapeutics to mitigate M. haemolytica infection in feedlot cattle. IMPORTANCE Bovine respiratory disease (BRD) is a significant animal health issue impacting the beef industry. Current BRD prevention strategies rely mainly on metaphylactic use of antimicrobials when cattle enter feedlots. However, a recent increase in BRD-associated bacterial pathogens that are resistant to metaphylactic antimicrobials highlights a pressing need for the development of novel mitigation strategies. Based upon previous research showing the importance of respiratory commensal bacteria in protecting against bronchopneumonia, this study aimed to develop bacterial therapeutics that could be used to mitigate the BRD pathogen Mannheimia haemolytica. Bacteria isolated from the respiratory tracts of healthy cattle were characterized for their inhibitory, adhesive, and immunomodulatory properties. In total, 6 strains were identified as having the best properties for use as intranasal therapeutics to inhibit M. haemolytica. If successful in vivo, these strains offer an alternative to metaphylactic antimicrobial use in feedlot cattle for mitigating BRD.

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Cattle Review

Livestock ◽

10.12968/live.2020.25.2.76 ◽

2020 ◽

Vol 25 (2) ◽

pp. 76-76

Author(s):

David C Barrett

Keyword(s):

Open Access ◽

Antimicrobial Resistance ◽

Respiratory Disease ◽

Bovine Tuberculosis ◽

Bovine Respiratory Disease ◽

Dairy Herds ◽

Digital Dermatitis ◽

Selection For

Introduction: In this Cattle Review we consider three open access papers on the association between antimicrobial class selection for treatment and retreatment of bovine respiratory disease and the development of antimicrobial resistance, between- and within-species transmission of bovine tuberculosis, and digital dermatitis in grazing dairy herds.

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