A turn-on fluorescent probe via substitution-rearrangement for highly sensitive and discriminative detection of cysteine and its imaging in living cells
A highly sensitive and selective fluorescent probe (FS) is based on a fluoride-specific desilylation reaction to yield a significant green fluorescence enhancement.
A novel and simple turn-on fluorescent probe bearing NBD and benzaldehyde fragments for the rapid optical sensing of biothiols has been designed and synthesized.
A new green-emitting fluorescent probe 1 was developed for biothiol detection. The sensing mechanism was considered to be biothiol-induced cleavage of the 2,4-dinitrobenzene- sulfonate group in probe 1 and resulting inhibition of the probe’s photoinduced electron transfer (PET) process. Probe 1 exhibited favorable properties such as excellent selectivity, highly sensitive (0.12 µM), large Stokes shift (117 nm) and a remarkable turn-on fluorescence signal (148-fold). Furthermore, confocal fluorescence imaging indicated that probe 1 was membrane-permeable and suitable for visualization of biothiols in living A549 cells.
Herein, we described a probe for H2S detection based on the reduction of azide to amine. This probe was highly sensitive and selective toward H2S and successfully applied for H2S fluorescent imaging in living cells.
Development of highly sensitive and selective fluorescent sensors toward Cu2+ has gained considerable attention in view of its application of environmental and biological fields. However, the strategy of sensing by...
Rhodamine B thiohydrazide (RBS) was firstly employed as turn-on fluorescent probe for hypochlorite in aqueous solution and living cells. It exhibits a stable response to hypochlorite from 1.0×10-6to 1.0×10-5M with a detection limit of 3.3×10-7M. The response of this probe to hypochlorite is fast and highly selective compared with other reactive oxygen species (such as.OH,1O2, H2O2) and other common anions (such as X-, ClO2-, ClO4-, NO3-, NO2-, OH-, Ac-, CO32-, SO42-).
A Green-emitting Fluorescent Probe Based on a Benzothiazole Derivative for Imaging Biothiols in Living Cells