Comparison of Rhodamine 6G, Rhodamine B and Rhodamine 101 Spirolactam Based Fluorescent Probes: A Case of pH Detection

2021 ◽  
pp. 120662
Author(s):  
Fei Deng ◽  
Dongsheng Sun ◽  
Shixu Yang ◽  
Wei Huang ◽  
Chunfang Huang ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Rhodamine B ◽  
Rhodamine 6G ◽  
Rhodamine 101 ◽  
Ph Detection

scholarly journals Hydrophobic analogues of rhodamine B and rhodamine 101: potent fluorescent probes of mitochondria in living C. elegans

2012 ◽  
Vol 8 ◽  
pp. 2156-2165 ◽  
Author(s):  
Laurie F Mottram ◽  
Safiyyah Forbes ◽  
Brian D Ackley ◽  
Blake R Peterson
Keyword(s):  
Fluorescent Probes ◽  
Rhodamine B ◽  
Laser Scanning ◽  
Fluorescent Dyes ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
C Elegans ◽  
Wide Range ◽  
Rhodamine 101

Mitochondria undergo dynamic fusion and fission events that affect the structure and function of these critical energy-producing cellular organelles. Defects in these dynamic processes have been implicated in a wide range of human diseases including ischemia, neurodegeneration, metabolic disease, and cancer. To provide new tools for imaging of mitochondria in vivo, we synthesized novel hydrophobic analogues of the red fluorescent dyes rhodamine B and rhodamine 101 that replace the carboxylate with a methyl group. Compared to the parent compounds, methyl analogues termed HRB and HR101 exhibit slightly red-shifted absorbance and emission spectra (5–9 nm), modest reductions in molar extinction coefficent and quantum yield, and enhanced partitioning into octanol compared with aqueous buffer of 10-fold or more. Comparison of living C. elegans (nematode roundworm) animals treated with the classic fluorescent mitochondrial stains rhodamine 123, rhodamine 6G, and rhodamine B, as well as the structurally related fluorophores rhodamine 101, and basic violet 11, revealed that HRB and HR101 are the most potent mitochondrial probes, enabling imaging of mitochondrial motility, fusion, and fission in the germline and other tissues by confocal laser scanning microscopy after treatment for 2 h at concentrations as low as 100 picomolar. Because transgenes are poorly expressed in the germline of these animals, these small molecules represent superior tools for labeling dynamic mitochondria in this tissue compared with the expression of mitochondria-targeted fluorescent proteins. The high bioavailabilty of these novel fluorescent probes may facilitate the identification of agents and factors that affect diverse aspects of mitochondrial biology in vivo.

Luminescence Quenching of Rhodamines by Cyclooctatetraene

1987 ◽  
Vol 42 (9) ◽  
pp. 1009-1013 ◽  
Author(s):  
P. Targowski ◽  
B. Ziętek ◽  
A. Bączyński
Keyword(s):  
Rhodamine B ◽  
Rate Constants ◽  
Internal Conversion ◽  
Rhodamine 6G ◽  
Second Order ◽  
Luminescence Quenching ◽  
Intersystem Crossing ◽  
Order Rate ◽  
Quenching Process

Cyclooctatetraene (COT) as a quencher of fluorescence of a series of Rhodamine solutions was studied. The second order rate constants for the quenching process of Rhodamine 110, Rhodamine 19 pchl., Rhodamine 6G pchl., Rhodamine 6G, Tetramethylrhodamine, Rhodamine B and Rhodamine 3B pchl. are given. It was found that COT enhances rather intersystem crossing than internal conversion.

Manipulating Energy Transfer Processes between Rhodamine 6G and Rhodamine B in Different Mesoporous Hosts

2007 ◽  
Vol 111 (14) ◽  
pp. 5541-5548 ◽  
Author(s):  
Lingzhi Wang ◽  
Yuanlin Liu ◽  
Feng Chen ◽  
Jinlong Zhang ◽  
Masakazu Anpo
Keyword(s):  
Energy Transfer ◽  
Rhodamine B ◽  
Rhodamine 6G ◽  
Transfer Processes

Facile Synthesis of Boron Organic Polymers for Efficient Removal and Separation of Methylene Blue, Rhodamine B, and Rhodamine 6G

2018 ◽  
Vol 6 (12) ◽  
pp. 16777-16787 ◽  
Author(s):  
Xue Zhao ◽  
Dan Wang ◽  
Changjun Xiang ◽  
Fulin Zhang ◽  
Luchang Liu ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Rhodamine B ◽  
Rhodamine 6G ◽  
Organic Polymers ◽  
Efficient Removal ◽  
Facile Synthesis

scholarly journals Toward an Accurate Spectrophotometric Evaluation of the Efficiencies of Photocatalysts in Processes Involving Their Separation Using Nylon Membranes

Catalysts ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 576
Author(s):  
Halema Al-Kandari ◽  
Peter Kasak ◽  
Ahmed Mohamed ◽  
Shekhah Al-Kandari ◽  
Dusan Chorvat Jr. ◽  
...  
Keyword(s):  
Rhodamine B ◽  
Significant Interaction ◽  
Rhodamine 6G ◽  
Solid Particles ◽  
Phenol Red ◽  
Model Compounds ◽  
Filtration Process ◽  
Nylon Membrane ◽  
Membrane Filters ◽  
Photocatalytic Reactors

Many works include the use of nylon membranes to separate the solid particles of photocatalysts from the photocatalytic reactors, before using spectrophotometers to evaluate the catalysts’ performance in the photocatalytic degradation of many pollutants. This might lead to significant errors due to the adsorption of some pollutants within the structure of the membranes during the filtration process used to separate the solid particles of the photocatalysts to get a clear filtrate. This, consequently, leads to incorrect calculations, which in turn are translated into false high photocatalytic efficiencies of the used catalysts. In this work, the authors study the interaction between nylon membrane filters and five different model compounds—phenol red, methylene blue, rhodamine B, rhodamine 6G, and phenol. The study reveals a significant interaction between the nylon membranes and both rhodamine B and phenol red.

Influence of laser and material parameters on two photon absorption in Rhodamine B and Rhodamine 6G solutions in MeOH

Optik ◽  
2019 ◽  
Vol 183 ◽  
pp. 835-841 ◽  
Author(s):  
Rabia Ahmad ◽  
M. Shahid Rafique ◽  
Ali Ajami ◽  
Shazia Bashir ◽  
Wolfgang Husinsky ◽  
...  
Keyword(s):  
Rhodamine B ◽  
Rhodamine 6G ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Material Parameters ◽  
Two Photon
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