Yeasts and Bacillus spp. as potential biocontrol agents of Sclerotinia sclerotiorum in garlic

2020 ◽  
Vol 261 ◽  
pp. 108931 ◽  
Author(s):  
Vytória Piscitelli Cavalcanti ◽  
Neilton Antonio Fiusa Araújo ◽  
Natália Bernardes Machado ◽  
Paulo Sérgio Pedroso Costa Júnior ◽  
Moacir Pasqual ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Biocontrol Agents ◽  
Bacillus Spp

Exploration of Endophytes from Alfalfa (Medicago sativa L.) as Biocontrol Agents

2020 ◽  
pp. 1-8
Author(s):  
Beilei Wu ◽  
Beilei Wu ◽  
Mei Li ◽  
Xiaoli Chen ◽  
Xiliang Jiang ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Root Rot ◽  
Agricultural Production ◽  
Biocontrol Agents ◽  
Bacillus Spp ◽  
Medicago Sativa L ◽  
The Common ◽  
Seedling Germination ◽  
Germination Experiments ◽  
Alfalfa Root

Endophytes are increasingly investigated as biocontrol agents for agricultural production. The identification of new endophytes with high effectiveness against plant disease is very important. A total of 362 strains of endophytes, including fungi, bacteria, and actinomycete, were isolated from alfalfa (Medicago sativa L.) collected in Hebei, Inner Mongolia and Ningxia provinces of China. The three strains of endophytic bacteria (NA NX51R-5, NA NX90R-8, and NA NM1S-1) with strong biocontrol capability with >50% effectiveness were screened against the common alfalfa root rot pathogen Fusarium oxysporum F. sp. medicaginis in alfalfa seedling germination experiments on MS medium and pot experiments. Using phylogenetic analysis, the isolates of NA NM1S-1 and NA NX51R-5 were identified as Bacillus spp. by 16S rDNA, while NA NX90R-8 was found to be Pseudomonas sp.

scholarly journals The Significance of Bacillus spp. in Disease Suppression and Growth Promotion of Field and Vegetable Crops

2020 ◽  
Vol 8 (7) ◽  
pp. 1037 ◽  
Author(s):  
Dragana Miljaković ◽  
Jelena Marinković ◽  
Svetlana Balešević-Tubić
Keyword(s):  
Plant Growth ◽  
Plant Pathogens ◽  
Growth Promotion ◽  
Disease Suppression ◽  
Biocontrol Agents ◽  
Systemic Resistance ◽  
Steady Increase ◽  
Vegetable Crops ◽  
Wide Range ◽  
Bacillus Spp

Bacillus spp. produce a variety of compounds involved in the biocontrol of plant pathogens and promotion of plant growth, which makes them potential candidates for most agricultural and biotechnological applications. Bacilli exhibit antagonistic activity by excreting extracellular metabolites such as antibiotics, cell wall hydrolases, and siderophores. Additionally, Bacillus spp. improve plant response to pathogen attack by triggering induced systemic resistance (ISR). Besides being the most promising biocontrol agents, Bacillus spp. promote plant growth via nitrogen fixation, phosphate solubilization, and phytohormone production. Antagonistic and plant growth-promoting strains of Bacillus spp. might be useful in formulating new preparations. Numerous studies of a wide range of plant species revealed a steady increase in the number of Bacillus spp. identified as potential biocontrol agents and plant growth promoters. Among different mechanisms of action, it remains unclear which individual or combined traits could be used as predictors in the selection of the best strains for crop productivity improvement. Due to numerous factors that influence the successful application of Bacillus spp., it is necessary to understand how different strains function in biological control and plant growth promotion, and distinctly define the factors that contribute to their more efficient use in the field.

scholarly journals Diversity of soil-dwelling Trichoderma in Colombia and their potential as biocontrol agents against the phytopathogenic fungus Sclerotinia sclerotiorum (Lib.) de Bary

2012 ◽  
Vol 79 (1) ◽  
pp. 74-85 ◽  
Author(s):  
Alexander Smith ◽  
Camilo A. Beltrán ◽  
Manabu Kusunoki ◽  
Alba M. Cotes ◽  
Keiichi Motohashi ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Biocontrol Agents ◽  
Phytopathogenic Fungus

Characterization of the anti-fungal activity of a Bacillus spp. associated with sclerotia from Sclerotinia sclerotiorum

2006 ◽  
Vol 72 (4) ◽  
pp. 644-653 ◽  
Author(s):  
Xingwei Hou ◽  
Susan M. Boyetchko ◽  
Myrtle Brkic ◽  
Doug Olson ◽  
Andrew Ross ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Fungal Activity ◽  
Bacillus Spp ◽  
Anti Fungal

scholarly journals New isolates of Trichoderma antagonistic to Sclerotinia sclerotiorum

2016 ◽  
Vol 16 (3) ◽  
Author(s):  
Eder Marques ◽  
Irene Martins ◽  
Mariana de Oliveira Cardoso Cunha ◽  
Marcello Arrais Lima ◽  
João Batista Tavares da Silva ◽  
...  
Keyword(s):  
Biological Control ◽  
Information System ◽  
Genetic Resources ◽  
Sclerotinia Sclerotiorum ◽  
Antagonistic Activity ◽  
Biocontrol Agents ◽  
Complete Inhibition ◽  
Its Sequences ◽  
White Mold

Abstract Forty-nine isolates of Trichoderma from the Brazilian Midwest were evaluated for their antagonistic activity in vitro against Sclerotinia sclerotiorum (causal agent of white mold), which were then identified based on their nuclear ribosomal ITS sequences. Paired culture tests showed that all isolates exhibited some antagonism, with a maximum of 77% mycelial inhibition and complete inhibition of sclerotia production. Two isolates were found to be the most promising biocontrol agents, considering both antagonistic parameters (CEN1253 - T. koningiopsis and CEN1265 - T. brevicompactum). Five different species were identified: T. harzianum (23), T. spirale (9), T. koningiopsis (8), T. brevicompactum (7) and T. asperellum (2). These isolates are stored in the Embrapa Fungi Collection for Biological Control and the information obtained in the experiments will be incorporated into the database of biological assets within the genetic resources information system (Allele) and be made available for further studies.

scholarly journals Bacillus spp. as Biocontrol Agents of Root Rot and Phytophthora Blight on Ginseng

2004 ◽  
Vol 20 (1) ◽  
pp. 63-66 ◽  
Author(s):  
Yeoung-Seuk Bae ◽  
Kyungseok Park ◽  
Choong-Hoe Kim
Keyword(s):  
Root Rot ◽  
Biocontrol Agents ◽  
Phytophthora Blight ◽  
Bacillus Spp

scholarly journals In vitro antifungal potential of Bacillus spp.: Isolates as biocontrol agents

2015 ◽  
pp. 163-180 ◽  
Author(s):  
Ivica Dimkic ◽  
Tatjana Stevic ◽  
Tanja Beric ◽  
Ivan Nikolic ◽  
Tamara Janakiev ◽  
...  
Keyword(s):  
Biocontrol Agents ◽  
Antifungal Potential ◽  
Bacillus Spp

scholarly journals Volatile Compounds of Endophytic Bacillus spp. have Biocontrol Activity Against Sclerotinia sclerotiorum

2018 ◽  
Vol 108 (12) ◽  
pp. 1373-1385 ◽  
Author(s):  
Venance Colman Massawe ◽  
Alvina Hanif ◽  
Ayaz Farzand ◽  
David Kibe Mburu ◽  
Sylvans Ochieng Ochola ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Morphological Changes ◽  
Antagonistic Activity ◽  
In Vitro Assays ◽  
Gas Chromatography Mass Spectrometry ◽  
Bromophenol Blue ◽  
Fungal Mycelium ◽  
Negative Effects ◽  
Bacillus Spp

To develop an effective biological agent to control Sclerotinia sclerotiorum, three endophytic Bacillus spp. strains with high antagonistic activity were isolated from maize seed and characterized. In vitro assays revealed that the Bacillus endophytes could produce volatile organic compounds (VOC) that reduced sclerotial production and inhibited mycelial growth of S. sclerotiorum. Gas chromatography–mass spectrometry revealed that the selected strains produced 16 detectable VOC. Eight of the produced VOC exhibited negative effects on S. sclerotiorum, while a further four induced accumulation of reactive oxygen species in mycelial cells. A mixture of VOC produced by Bacillus velezensis VM11 caused morphological changes in the ultrastructure and organelle membranes of S. sclerotiorum mycelial cells. The bromophenol blue assay revealed a yellow color of untreated fungal mycelium, which grew faster and deeper from 24 to 72 h postinoculation, as an indication of reduced pH. The potassium permanganate (KMnO4) titration assay showed that the rate of oxalic acid accumulation was higher in minimal salt liquid medium cultures inoculated with untreated fungal plugs compared with the Bacillus VOC-treated ones. Interestingly, biological control assays using host-plant leaves challenged with treated fungal mycelial plugs produced reduced lesions compared with the control. These findings provide new viable possibilities of controlling diseases caused by S. sclerotiorum using VOC produced by Bacillus endophytes.

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