The article presents the results of work on conservation and study of grapevine genetic resources at the Don ampelographic collec-tion named afterYa.I.Potapenko (Novocher-kassk, Rostov region) in 2020. In the context of global and local climate change, the most relevant direction in the development of modern viticulture is the improvement of the grape assortment by introducing varieties that are resistant to adverse environmental factors and more fully realize their productivity po-tential in a particular growing region. The purpose of the research is to preserve (with annual replenishment) and study the gene pool of grapes of various ecological and geo-graphical origin in the collection. The distri-bution of varieties by ripening terms, produc-tivity, uvological indicators has been carried out. 53 varieties of grapes were transferred for micro-winemaking, of which 23 are na-tive Don varieties. In 2020, the collection's gene pool was replenished with 5 grape varie-ties (Baikonur, Galahad, Meskhei,Novyj Po-darok Zaporozhyu, Kishmish Novocher-kassky); to preserve the gene pool, replanting and repairs were carried out (by the method of green inoculation in place). The varieties - Tsimlyanskiy Sergienko and Bessergenevskiy No. 7 were identified as valuable genotypes with high technological properties for high-quality winemaking; the database was sup-plemented with ampelographic descriptions of 50 grape varieties growing on the collec-tion.