Natural green deep eutectic solvents-based eco-friendly and efficient extraction of flavonoids from Selaginella moellendorffii: Process optimization, composition identification and biological activity

A novel, efficient extraction procedure based on natural deep eutectic solvents (NADES) and ionic liquids (ILs) for determination of 20-hydroxyecdysone (20-E) in spinach has been developed. NADES, the first green extraction agent, with different hydrogen bond donors and acceptors are screened in order to determine extraction efficiencies. NADES consisting of lactic acid and levulinic acid at a molar ratio of 1:1 exhibits the highest yields. ILs, the second green extraction agent, with various cations and anions are also investigated, where [TEA] [OAc]·AcOH, χAcOH = 0.75 displays the highest recovery. Moreover, NADES-SLE and IL-SLE (SLE, solid-liquid extraction) parameters are investigated. Using the obtained optimized method, the recoveries of the target compound in spinach are above 93% and 88% for NADES-SLE and IL-SLE procedure, respectively. The methods display good linearity within the range of 0.5–30 μg/g and LODs of 0.17 µg/g. The proposed NADES-SLE-UHPLC-UV and IL-SLE-UHPLC-UV procedures can be applied to the analysis of 20-E in real spinach samples, making it a potentially promising technique for food matrix. The main advantage of this study is the superior efficiency of the new, green extraction solvents, which results in a significant reduction of extraction time and solvents as compared to those in the literature.

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Green and Efficient Extraction of Polysaccharides From Poria cocos F.A. Wolf by Deep Eutectic Solvent

Natural Product Communications ◽

10.1177/1934578x19900708 ◽

2020 ◽

Vol 15 (2) ◽

pp. 1934578X1990070 ◽

Cited By ~ 1

Author(s):

Weida Zhang ◽

Shaobo Cheng ◽

Xiaona Zhai ◽

Junshe Sun ◽

Xuefang Hu ◽

...

Keyword(s):

Choline Chloride ◽

Deep Eutectic Solvent ◽

Deep Eutectic Solvents ◽

Extraction Yield ◽

Hot Water ◽

Poria Cocos ◽

Extraction Solvent ◽

Molecular Weight Distributions ◽

Efficient Extraction ◽

Run Test

Deep eutectic solvents (DESs) were proposed for the extraction of polysaccharides from Poria cocos (PCPs). Six types of DESs were prepared, and the DES composed of choline chloride and oxalic acid was proved to be suitable. Based on the results of single-factor test, the Box-Behnken experimental design with response surface methodology was carried out, giving the optimal extraction conditions including mole ratio of 1:2 (choline chloride:oxalic acid) and extraction 15 minutes at 100°C. Under the optimal extraction conditions, the extraction yield (46.24% ± 0.13%) was 8.6 times higher than that of hot water. The reusability of DES was demonstrated by a 6-run test, and an extraction yield of PCP was 38.40% ± 0.23% after reusing for 6 times without adding any additional chemicals. Moreover, molecular weight distributions of the resulting PCP were analyzed, and then mainly distributed in the range of 753 to 3578 g/mol. Therefore, DESs were proved to be an excellent extraction solvent alternative to the extraction of PCP.

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Efficient extraction of major catechins in Camellia sinensis leaves using green choline chloride-based deep eutectic solvents

RSC Advances ◽

10.1039/c5ra15830c ◽

2015 ◽

Vol 5 (114) ◽

pp. 93937-93944 ◽

Cited By ~ 36

Author(s):

Ji Li ◽

Zhigang Han ◽

Yongpeng Zou ◽

Bo Yu

Keyword(s):

Organic Acids ◽

Camellia Sinensis ◽

Choline Chloride ◽

Deep Eutectic Solvents ◽

Green Solvents ◽

Efficient Extraction

Choline chloride-based deep eutectic solvents (DESs), composed of alcohols, organic acids and saccharides, were used as green solvents for extraction of major catechins inCamellia sinensisleaves.

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Novel perspective on botanical extraction – Natural deep eutectic solvents (NaDES) as enhancers of biological activity

Planta Medica ◽

10.1055/s-0036-1597001 ◽

2016 ◽

Vol 81 (S 01) ◽

pp. S1-S381

Author(s):

A Lavaud ◽

M Laguerre ◽

C Szewezyk ◽

A Bily

Keyword(s):

Biological Activity ◽

Deep Eutectic Solvents ◽

Natural Deep Eutectic Solvents

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Green and Efficient Extraction of Resveratrol from Peanut Roots Using Deep Eutectic Solvents

Journal of Chemistry ◽

10.1155/2018/4091930 ◽

2018 ◽

Vol 2018 ◽

pp. 1-9 ◽

Cited By ~ 2

Author(s):

Jingnan Chen ◽

Xingxing Jiang ◽

Guolong Yang ◽

Yanlan Bi ◽

Wei Liu

Keyword(s):

Choline Chloride ◽

Deep Eutectic Solvents ◽

Extraction Yield ◽

Box Behnken Design ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Efficient Extraction ◽

Ultrasonic Assisted Extraction ◽

Solid Liquid ◽

Total Extraction

Deep eutectic solvents (DESs), a new group of ecofriendly solvent combined with the ultrasonic-assisted extraction (UAE) technique, were first successfully used for extraction of resveratrol from peanut roots. Resveratrol in the extract was analyzed and quantified using a HPLC-UV method. A series of DESs consisting of choline chloride (ChCl) and 1,4-butanediol, citric acid, and ethylene glycol were formulated, finding ChCl/1,4-butanediol was a most proper extraction system. The optimal extraction parameters were obtained using a Box–Behnken design (BBD) test combined with response surface methodology as follows: 40% of water in ChCl/1,4-butanediol (1/3, g/g) at 55°C for 40 min and solid/liquid ratio of 1:30 g/mL. The total extraction content and extraction yield of resveratrol from peanut roots could reach 38.91 mg/kg and 88.19%, respectively, under such optimal conditions. The present study will provide a typical example for using DESs to extract natural bioactive compounds from plants.

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