Carcass traits, meat quality characteristics, and lipid metabolism-related gene expression pattern of Yaoshan white goats raised in traditional extensive production system: Effects of slaughter age and meat cuts

2020 ◽  
Vol 182 ◽  
pp. 29-36
Author(s):  
Ningning Zhang ◽  
Zhanwei Teng ◽  
Qin Qi ◽  
Gaojie Hu ◽  
Hongxia Lian ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lipid Metabolism ◽  
Expression Pattern ◽  
Meat Quality ◽  
Production System ◽  
Carcass Traits ◽  
Gene Expression Pattern ◽  
Quality Characteristics ◽  
Related Gene ◽  
Slaughter Age

scholarly journals Lipid metabolism-related gene expression pattern of Atlantic bluefin tuna (Thunnus thynnus L.) larvae fed on live prey

2016 ◽  
Vol 43 (2) ◽  
pp. 493-516 ◽  
Author(s):  
Mónica B. Betancor ◽  
Aurelio Ortega ◽  
Fernando de la Gándara ◽  
Douglas R. Tocher ◽  
Gabriel Mourente
Keyword(s):  
Gene Expression ◽  
Lipid Metabolism ◽  
Expression Pattern ◽  
Gene Expression Pattern ◽  
Bluefin Tuna ◽  
Related Gene ◽  
Thunnus Thynnus ◽  
Live Prey ◽  
Atlantic Bluefin Tuna

Carcass Traits and Meat Quality Characteristics of Malabari Male Kids Reared under Different Production System

2020 ◽  
Author(s):  
Chikkagoudara Kotresh Prasad ◽  
John Abraham ◽  
Balusami Chinnappan ◽  
Roshin Anie Jose ◽  
Senthil Murugan ◽  
...  
Keyword(s):  
Body Weight ◽  
Meat Quality ◽  
Production System ◽  
Carcass Traits ◽  
Live Weight ◽  
Quality Characteristics ◽  
Cooking Loss ◽  
Body Parts ◽  
Final Body Weight ◽  
The Impact

Background: The intensive rearing systems of goats needs dietary formulations that will meet animal requirements, mainly the protein and energy to allow optimum growth rate. Alternative systems to attain 15-20 kg body weight by three months of age with better meat quality is need of the hour. Broiler goat production system is most suitable system of rearing for meat goat production. The current study was conducted to examine the impact of broiler goat production system on the carcass traits and meat quality characteristics of Malabari male kids. Methods: Fourteen pre-weaned kids were randomly allotted to two treatments. Treatment-I (T1) kids were allowed to suckle thrice a day during four weeks and twice in a day during consequent weeks. Whereas in Treatment-II (T2), kids were reared intensively by providing finely ground concentrate feed mixed with equal quantity of rice gruel from a feeding bottle after 7 days of colostrum feeding from the mother. The animals were slaughtered at an age of three months and the final body weight, carcass traits like dressing percentage, weight of different body parts and meat quality parameters like pH, color, cooking loss and shear force were analysed. The proximate composition and sensory evaluation was performed. Result: Broiler goat production system could produce significantly (P less than 0.01) higher final average body weight compared to natural system. All the carcass traits were significantly (P less than 0.01) higher in T2. The broiler goat production system could double the net live weight during the same period compared to natural suckling system with higher dressing % and the chevon produced was tender with less cooking loss. Hence, this system can be recommended for chevon production in shorter period with higher dressing % and lesser cooking loss in Malabari male kids.

Comparison of even-skipped related gene expression pattern in vertebrates shows an association between expression domain loss and modification of selective constraints on sequences

2003 ◽  
Vol 5 (2) ◽  
pp. 145-156 ◽  
Author(s):  
Fabien Avaron ◽  
Christelle Thaeron-Antono ◽  
Caroline W. Beck ◽  
Veronique Borday-Birraux ◽  
Jacqueline Geraudie ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Pattern ◽  
Gene Expression Pattern ◽  
Related Gene ◽  
Expression Domain ◽  
Selective Constraints ◽  
Domain Loss

scholarly journals Probiotics or Synbiotics Addition in Bama Mini-pigs’ Diets Improves Growth Performance, Carcass Traits, and Meat Quality by Altering Plasma Metabolites and Related Gene Expression of Offspring Pigs

2021 ◽  
Author(s):  
Qian Zhu ◽  
Mingtong Song ◽  
Md. Abul Kalam Azad ◽  
Cui Ma ◽  
Yulong Yin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Performance ◽  
Meat Quality ◽  
Shear Force ◽  
Carcass Traits ◽  
Backfat Thickness ◽  
Plasma Metabolites ◽  
Related Gene ◽  
Gene Expressions ◽  
Mini Pigs

Abstract Background: The present study evaluated the effects of maternal probiotics or synbiotics addition on growth performance, carcass traits, meat quality, plasma metabolites, and related gene expression of their offspring pigs. Methods: A total of 64 Bama mini-pigs were used and randomly divided into the control, antibiotic, probiotics, or synbiotics groups, and fed experimental diets during pregnancy and lactation. After weaning, two piglets per litter and eight piglets per group were selected and fed a basal diet. At 65-, 95-, and 125-day-old, eight pigs per group were selected for analysis. Results: The results showed that probiotics addition increased the ADFI of pigs during the 66-95 day-old and the backfat thickness at 65- and 125-day-old, whereas synbiotics addition increased backfat thickness and decreased muscle percentage and loin-eye area at 125-day-old. In meat quality, probiotics or synbiotics addition increased cooking yield, pH 45min , and meat color, while decreased drip loss and shear force at different stages. However, maternal antibiotic addition showed an increased shear force at 125-day-old. Dietary probiotics or synbiotics addition in sow diets increased several amino acids (AA) contents in the plasma and longissimus thoracis (LT) muscle, including total AA (TAA), His, Met, Asp, Arg, and Leu, while decreased Gly, Pro, Ile, α-AAA, α-ABA, β-Ala, and γ-ABA at different stages. In the LT muscle fatty acid (FA) analysis, the contents of saturated FA (SFA; including C16:0, C17:0, and C20:0) and C18:1n9t were decreased, and the contents of C18:2n6c, MUFA (C16:1 and C20:1) and UFA were increased in the probiotics group, while the contents of SFA (C10:0, C12:0, and C14:0 at 65-day-old) were increased and the contents of C20:1 and C18:1n9t were decreased in the synbiotics group. Plasma biochemical analysis revealed that maternal probiotics or synbiotics addition decreased plasma AMM, UN, and GLU, while synbiotics addition increased plasma ALT, HDL-C, and TC at different stages. Moreover, maternal probiotics or synbiotics regulated muscle fiber-type, myogenic regulatory, and lipid metabolism-related gene expressions of offspring pigs. Conclusions: In conclusion, maternal probiotics or synbiotics addition, as a nutritional intervention strategy, improved feed intake and meat quality by altering the body's metabolism of offspring pigs and gene expressions related to meat quality.

scholarly journals 82 EFFECT OF HIGH FETAL CALF SERUM CONCENTRATION IN THE GENE EXPRESSION PATTERN OF IN VITRO PRODUCED BOVINE EMBRYOS

2014 ◽  
Vol 26 (1) ◽  
pp. 155
Author(s):  
M. J. Sudano ◽  
D. M. Paschoal ◽  
E. S. Caixeta ◽  
R. R. Maziero ◽  
M. D. Guastali ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Death ◽  
Lipid Metabolism ◽  
Expression Pattern ◽  
Expression Profiles ◽  
Rna Extraction ◽  
Gene Expression Pattern ◽  
Differentially Expressed ◽  
Bovine Embryos

Even though FCS provides energy substrates, amino acids, vitamins, growth factors, and heavy-metal chelators, its supplementation has been associated with several embryo abnormalities such as mitochondrial degeneration, metabolic deviations, excessive lipid accumulation, and decreased embryo survival after cryopreservation. The aim of the present study was to evaluate the effect of high FCS concentration in the gene expression pattern of in vitro-produced bovine embryos. Slaughterhouse ovaries were used to obtain oocytes (N = 360), which were matured and fertilized in vitro (Day 0). Presumptive zygotes were divided in 2 culture media: with low (SOFaa with 0.5% BSA and 2.5% FCS) or high (SOFaa with 0.5% BSA and 10% FCS) FCS concentration. Cleavage was evaluated on Day 3. Embryo development was evaluated after 7 days under standard culture conditions (at 38.5°C in atmosphere of 5% O2, 5% CO2, and 90% N2). The produced blastocysts were placed in PBS solution and washed five times. A single blastocyst was frozen in a minimal volume of PBS and stored at –80°C until RNA extraction. Total RNA extraction was performed using the PicoPure RNA isolation Kit (Applied Biosystems®, Foster City, CA, USA). Extracted RNA was evaluated through 2100-Bioanalyzer (Agilent Technologies®, Palo Alto, CA, USA) and DNAse treated (Qiagen®, Valencia, CA, USA). RiboAmp RNA Amplification Kit (Applied Biosystems®) was used to amplify the RNA (T7 RNA polymerase-catalysed amplification reaction). The aRNA output was evaluated through NanoDrop ND-1000 (NanoDrop Technologies®, Wilmington, DE, USA). A biotin-labelled cRNA and fragmented cRNA were obtained through 3′IVT Express Kit (Affymetrix®, Santa Clara, CA, USA) to perform the hybridization (N = 3 per group) using GeneChip Bovine Genome Array (Affymetrix®). Following hybridization, probe arrays were washed, stained, and scanned. Microarray data analysis was performed in the software FlexArray 1.6.1.1. Genes with a fold change of at least 1.5 and a probability of P < 0.05 were considered differentially expressed. The data from in vitro embryo production were analysed through the PROC GLM (SAS Institute Inc., Cary, NC, USA). Cleavage rate (81.4 ± 1.5 and 85.5 ± 1.4) and blastocyst production (41.8 ± 2.4 and 47.2 ± 2.8) were not different (P > 0.05) between low and high FCS concentrations, respectively. A total of 40 genes were differentially expressed between low and high FCS concentration. A total of 28 genes were annotated, with 37 genes up-regulated and 3 genes down-regulated by high FCS concentration. The associated network functions of gene expression, RNA damage and repair, and post-transcriptional modification; and cell-to-cell signalling and interaction were generated by Ingenuity Pathway Analysis® (Redwood City, CA, USA). Differentially expressed genes involved in carbohydrate metabolism (GAPVD1, MGAT4A), lipid metabolism (ELOVL5), cellular assembly and organisation (EZR, LRP2), and cell death and survival (DRT8) were identified. In conclusion, high FCS supplementation was associated with different expression profiles of genes regulating carbohydrate and lipid metabolism, cellular assembly and organisation, and cell death and survival. The authors acknowledge support from FAPESP and LNBio-CNPEM.

scholarly journals Identification of a Five Autophagy Subtype-Related Gene Expression Pattern for Improving the Prognosis of Lung Adenocarcinoma

2021 ◽  
Vol 9 ◽  
Author(s):  
Meng-Yu Zhang ◽  
Chen Huo ◽  
Jian-Yu Liu ◽  
Zhuang-E. Shi ◽  
Wen-Di Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Pattern ◽  
Risk Model ◽  
Cox Regression ◽  
Gene Expression Pattern ◽  
Consensus Clustering ◽  
Regression Analyses ◽  
Related Gene ◽  
Predictive Risk ◽  
Clinicopathological Variables

Background: Autophagy plays an important role in lung adenocarcinoma (LUAD). In this study, we aimed to explore the autophagy-related gene (ARG) expression pattern and to identify promising autophagy-related biomarkers to improve the prognosis of LUAD.Methods: The gene expression profiles and clinical information of LUAD patients were downloaded from the Cancer Genome Atlas (TCGA), and validation cohort information was extracted from the Gene Expression Omnibus database. The Human Autophagy Database (HADb) was used to extract ARGs. Gene expression data were analyzed using the limma package and visualized using the ggplot2 package as well as the pheatmap package in R software. Functional enrichment analysis was also performed for the differentially expressed ARGs (DEARGs). Then, consensus clustering revealed autophagy-related tumor subtypes, and differentially expressed genes (DEGs) were screened according to the subtypes. Next, the univariate Cox and multivariate Cox regression analyses were used to identify independent prognostic ARGs. After overlapping DEGs and the independent prognostic ARGs, the predictive risk model was established and validated. Correlation analyses between ARGs and clinicopathological variables were also explored. Finally, the TIMER and TISIDB databases were used to further explore the correlation analysis between immune cell infiltration levels and the risk score as well as clinicopathological variables in the predictive risk model.Results: A total of 222 genes from the HADb were identified as ARGs, and 28 of the 222 genes were pooled as DEARGs. The most significant GO term was autophagy (p = 3.05E-07), and KEGG analysis results indicated that 28 DEARGs were significantly enriched in the ErbB signaling pathway (p &lt; 0.001). Then, consensus clustering analysis divided the LUAD into two clusters, and a total of 168 DEGs were identified according to cluster subtypes. Then univariate and multivariate Cox regression analyses were used to identify 12 genes that could serve as independent prognostic indicators. After overlapping 168 DEGs and 12 genes, 10 genes (ATG4A, BAK1, CAPNS1, CCR2, CTSD, EIF2AK3, ITGB1, MBTPS2, SPHK1, ST13) were selected for the further exploration of the prognostic pattern. Survival analysis results indicated that this risk model identified the prognosis (p = 4.379E-10). Combined with the correlation analysis results between ARGs and clinicopathological variables, five ARGs were screened as prognostic genes. Among them, SPHK1 expression levels were positively correlated with CD4+ T cells and dendritic cell infiltration levels.Conclusions: In this study, we constructed a predictive risk model and identified a five autophagy subtype-related gene expression pattern to improve the prognosis of LUAD. Understanding the subtypes of LUAD is helpful to accurately characterize the LUAD and develop personalized treatment.

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