Selection of breed-specific SNPs in three Indian sheep breeds using ovine 50 K array

2021 ◽  
Vol 205 ◽  
pp. 106545
Author(s):  
Harshit Kumar ◽  
Manjit Panigrahi ◽  
Divya Rajawat ◽  
Anuradha Panwar ◽  
Sonali Sonejita Nayak ◽  
...  
Keyword(s):  
Sheep Breeds ◽  
Selection Of ◽  
Indian Sheep

PSXII-2 Identification of candidate SNPs associated with high prolificacy in Romanov sheep

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 254-255
Author(s):  
Alexey V Shakhin ◽  
Arsen V Dotsev ◽  
Tatiana E Deniskova ◽  
Gottfried Brem ◽  
Natalia A Zinovieva
Keyword(s):  
Higher Education ◽  
Genetic Basis ◽  
Reference Genome ◽  
Unique Property ◽  
Candidate Snps ◽  
Merino Sheep ◽  
Sheep Breeds ◽  
Important Trait ◽  
Very High ◽  
Selection Of

Abstract Prolificacy is a very important trait in sheep. Romanov sheep, the well-known Russian sheep breed, are characterized by very high prolificacy; however, the genetic basis of this unique property of Romanov sheep is still unknown. It was reported that Ovine BMPR1B gene, located on the OAR6, is associated with prolificacy in several sheep breeds. The aim of our study was to identify candidate SNPs within BMPR1B gene, related to prolificacy. To achieve this goal, using NGS technology, we sequenced ovine BMPR1B gene in Romanov sheep (n = 6), which are characterized by high prolificacy (about 270 lambs per 100 ewes). The sequences of BMPR1B gene of Noire du Velay, Tan, Southdown and Australian Horned Merino sheep breeds as well as Asiatic mouflon (n = 1), which are characterized by significantly lower prolificacy (from 110 to 180 lambs per 100 ewes) were derived from publicly available sources and used for comparison. FST analysis performed in PLINK 1.9 program revealed 10 SNPs with values higher than 0.8. The majority of candidate SNPs under putative selection were localized in the region from 29,382,098 to 29,430,387 on OAR6 of Ovine reference genome (Oar_v3.1 (Ensembl release 98). Thus, we can suggest, that this region of the BMPR1B gene can be considered as the putative region, associated with high prolificacy of Romanov sheep. Additional studies will be needed to confirm the effect of identified candidate SNPs on prolificacy traits. The research results will be useful for artificial selection of sheep with higher prolific capacity, including the introduction of desired alleles in sheep populations using genome editing technologies. This work was supported by the Russian Ministry of Science and Higher Education No. 0445-2019-0024 and RFBR No. 20-516-56002.

scholarly journals Evidence for independent domestication of sheep mtDNA lineage A in India and introduction of lineage B through Arabian sea route

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ranganathan Kamalakkannan ◽  
Satish Kumar ◽  
Karippadakam Bhavana ◽  
Vandana R. Prabhu ◽  
Carolina Barros Machado ◽  
...  
Keyword(s):  
Arabian Sea ◽  
Indian Subcontinent ◽  
Domestic Sheep ◽  
Published Data ◽  
Sheep Population ◽  
Sheep Breeds ◽  
The World ◽  
History Of ◽  
Wild Sheep ◽  
Indian Sheep

AbstractIndia ranks the second in the world in terms of its sheep population with approximately 74.26 million represented by 44 well-described breeds in addition to several non-descript populations. Genetic diversity and phylogeography of Indian sheep breeds remain poorly understood, particularly for south Indian breeds. To have a comprehensive view of the domestication history of Indian sheep, we sequenced the mitochondrial DNA (mtDNA) control region (D-loop) and cytochrome b gene (CYTB) of 16 Indian domestic sheep breeds, most of them (13) from the south India. We analysed these sequences along with published data of domestic and wild sheep from different countries, including India. The haplotype diversity was relatively high in Indian sheep, which were classified into the three known mtDNA lineages, namely A, B and C. Lineage A was predominant among Indian sheep whereas lineages B and C were observed at low frequencies but C was restricted to the breeds of north and east India. The median joining network showed five major expanding haplogroups of lineage A (A1–A5). Out of which, A2, A4 and A5 were more frequent in Indian sheep in contrast to breeds from other parts of the world. Among the 27 Indian sheep breeds analysed, Mandya and Sonadi breeds were significantly different from other Indian breeds in the MDS analyses. This was explained by a very high contribution of lineage B into these two breeds. The Approximate Bayesian Computation (ABC) provided evidence for the domestication of lineage A sheep in the Indian subcontinent. Contrary to the current knowledge, we also found strong support for the introduction of lineage B into Indian subcontinent through sea route rather than from the Mongolian Plateau. The neighbour-joining tree of domestic and wild sheep revealed the close genetic relationship of Indian domestic sheep with Pakistani wild sheep O. vignei blanfordi. Based on our analyses and archaeological evidences, we suggest the Indian subcontinent as one of the domestication centres of the lineage A sheep, while lineage B sheep might have arrived into India from elsewhere via Arabian sea route. To the best of our knowledge, this is the first comprehensive study on Indian sheep where we have analysed more than 740 animals belonging to 27 sheep breeds raised in various regions of India. Our study provides insight into the understanding of the origin and migratory history of Indian sheep.

Polymorphism in Exon 17 of Diacylglycerol Acyltransferase-1{DGAT-1)Gene in Indian Sheep Breeds

2016 ◽  
Vol 22 (2) ◽  
pp. 170
Author(s):  
Rajiv Kumar ◽  
Shringarika Gupta ◽  
A.S. Meena ◽  
S.M.K. Naqvi ◽  
S. Kumar
Keyword(s):  
Diacylglycerol Acyltransferase ◽  
Sheep Breeds ◽  
Indian Sheep

RFLP analysis of mitochondrial D-loop region in Indian sheep breeds

2015 ◽  
Vol 21 (1) ◽  
pp. 13
Author(s):  
M.G. Sahare ◽  
A.D. Sawaimul ◽  
S.Z. Ali ◽  
A.R. Sirothia ◽  
S. Kumar
Keyword(s):  
Rflp Analysis ◽  
Sheep Breeds ◽  
Loop Region ◽  
D Loop ◽  
Indian Sheep

scholarly journals Sequence Characterization of Ovine MHC Class II (DRB I Gene) in Indian Sheep Breeds

2018 ◽  
Vol 7 (07) ◽  
pp. 490-499
Author(s):  
Kush Shrivastava ◽  
Pushpendra Kumar ◽  
Mohd. Faheem Khan ◽  
Shankar Dayal ◽  
Nihar Ranjan Sahoo ◽  
...  
Keyword(s):  
Mhc Class Ii ◽  
Class Ii ◽  
Sequence Characterization ◽  
Sheep Breeds ◽  
I Gene ◽  
Indian Sheep

scholarly journals Prevalence of SRLV infections in various sheep breeds

2018 ◽  
Vol 74 (8) ◽  
pp. 517-519
Author(s):  
WIKTOR BOJAR ◽  
ANDRZEJ JUNKUSZEW ◽  
MONIKA OLECH ◽  
JACEK KUŹMAK ◽  
KLAUDIUSZ SZCZEPANIAK
Keyword(s):  
Blood Serum ◽  
Elisa Test ◽  
Infection Prevalence ◽  
Breeding Strategies ◽  
Sample Collection ◽  
Serological Reaction ◽  
Specific Antibodies ◽  
Sheep Breeds ◽  
Selection Of

The aim of the study was to determine the prevalence of SRLV infection in sheep breeds farmed in mid-Eastern Poland. Out of 6,470 mother ewes kept in 98 nucleus flocks of various sizes, 2,924 belonging to 15 breeds and lines were selected for serum sample collection. The selection of the animals analyzed was carried out using stratification which enabled the determination of infection prevalence with an error margin no greater than 8%. The assay of SRLV-specific antibodies in blood serum was performed using the ELISA test. The analyses revealed a large diversity of prevalence between the sheep breeds studied. The lowest prevalence was observed in Uhruska sheep, in which only 5.07% had a positive serological reaction, despite the fact that the number of animals of this breed included in the analysis was large (1675 mother ewes). The prevalence for this variety was over 5-7 times lower in comparison to Wielkopolska sheep (35%), Polish Pogorze sheep (22.73%), Podhale Zackel sheep (29.03%) and over 10 times lower than Świniarka sheep (57.04%). The results should be used for developing breeding strategies and establishing anti-SRLV programs..

Genetic polymorphism in fecundity gene (FecG) among Indiansheep breeds Balangir, Shahabadi and Bonpala

2016 ◽  
Author(s):  
Jowel Debnath ◽  
Ran Vir Singh
Keyword(s):  
Litter Size ◽  
Transforming Growth Factor ◽  
Natural Habitat ◽  
Factor B ◽  
Sheep Breeds ◽  
Homozygous Genotype ◽  
Average Litter Size ◽  
Pcr Rflp ◽  
Selection Of ◽  
Gdf9 Gene

FecG (GDF9) is a member of the transforming growth factor-b (TGF- b) superfamily, have been shown to be essential for follicular growth and ovulation. Different mutations in FecG gene caused increased ovulation and infertility in sheep. The present study was designed for screening polymorphism of FecG gene in 250 selected ewes from different sheep flocks representing Balangir (100), Shahabadi (100) and Bonpala (50) by employing forced PCR-RFLP technique. Genomic DNA was extracted from the blood of Balangir, Shahabadi and Bonpala matured ewes with average litter size varying from 1.00± 0.00 to 1.14±0.02 at different parities. Digestion of FecG (GDF9) gene with DdeI restriction enzyme resulted into FecGHH homozygous genotype. In all three sheep breeds, genotypic frequencies of FecGHH were 100% and gene frequency of H allele was unity. This indicates that the FecG gene is fixed in the Balangir, Shahabadi and Bonpala population in the natural habitat. Litter size of Balangir and Bonpala sheep breeds were single but in Shahabadi sheep twin was recorded. In present study all the animals of three breeds were homozygous for FecG and there was no infertility observed in above mentioned breeds in field condition and organized farm, which is not in consonance with previous report. The observed effects could be caused by linkage disequilibrium with other nearby loci. The study revealed that FecG gene is not a reliable genetic marker for selection of high prolificacy in sheep.

Polymorphism of KRT 1.2 and KAP 1.3 Genes in Indian Sheep Breeds

2016 ◽  
Vol 22 (1) ◽  
pp. 28
Author(s):  
Rajiv Kumar ◽  
A.S. Meena ◽  
R. Kumari ◽  
B. Jyotsana ◽  
L.L.L. Prince ◽  
...  
Keyword(s):  
Sheep Breeds ◽  
Indian Sheep
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