Genetic polymorphism in fecundity gene (FecG) among Indiansheep breeds Balangir, Shahabadi and Bonpala

2016 ◽  
Author(s):  
Jowel Debnath ◽  
Ran Vir Singh
Keyword(s):  
Litter Size ◽  
Transforming Growth Factor ◽  
Natural Habitat ◽  
Factor B ◽  
Sheep Breeds ◽  
Homozygous Genotype ◽  
Average Litter Size ◽  
Pcr Rflp ◽  
Selection Of ◽  
Gdf9 Gene

FecG (GDF9) is a member of the transforming growth factor-b (TGF- b) superfamily, have been shown to be essential for follicular growth and ovulation. Different mutations in FecG gene caused increased ovulation and infertility in sheep. The present study was designed for screening polymorphism of FecG gene in 250 selected ewes from different sheep flocks representing Balangir (100), Shahabadi (100) and Bonpala (50) by employing forced PCR-RFLP technique. Genomic DNA was extracted from the blood of Balangir, Shahabadi and Bonpala matured ewes with average litter size varying from 1.00± 0.00 to 1.14±0.02 at different parities. Digestion of FecG (GDF9) gene with DdeI restriction enzyme resulted into FecGHH homozygous genotype. In all three sheep breeds, genotypic frequencies of FecGHH were 100% and gene frequency of H allele was unity. This indicates that the FecG gene is fixed in the Balangir, Shahabadi and Bonpala population in the natural habitat. Litter size of Balangir and Bonpala sheep breeds were single but in Shahabadi sheep twin was recorded. In present study all the animals of three breeds were homozygous for FecG and there was no infertility observed in above mentioned breeds in field condition and organized farm, which is not in consonance with previous report. The observed effects could be caused by linkage disequilibrium with other nearby loci. The study revealed that FecG gene is not a reliable genetic marker for selection of high prolificacy in sheep.

scholarly journals Variant <i>GDF9</i> mRNA is likely not the main cause of larger litter size in Iranian Lori-Bakhtyari, Shal, Ghezel, and Afshari sheep breeds

2017 ◽  
Vol 60 (2) ◽  
pp. 119-129 ◽  
Author(s):  
Shahin Eghbalsaied ◽  
Farzad Rashidi Khorasgani ◽  
Hamid-Reza Amini ◽  
Majid Farahi ◽  
Maryam Davari ◽  
...  
Keyword(s):  
Litter Size ◽  
Signal Peptide ◽  
Large Dataset ◽  
Sheep Breeds ◽  
Signal Peptide Region ◽  
Twin Birth ◽  
Significant Difference ◽  
Pcr Rflp ◽  
Peptide Region ◽  
Gdf9 Gene

Abstract. This study was carried out to screen the GDF9 gene and evaluate the polymorphism effect on litter size of four Iranian sheep breeds using the PCR-RFLP and PCR-SSCP methods. First, sequencing of the GDF9 gene in 16 twin-birth, 4 triplet-birth, and 2 infertile ewes showed that, in addition to G2, G3, G4, G5, and G6 mutations that have been previously reported in other breeds, a new G0 mutation, called C25T, exists in the GDF9 sequence of 1 out of 22 ewes and causes L9F substitution in the signal peptide region. None of the triplet-birth or infertile ewes carried G1, G4, G7, FecGE, G8, or FecGT mutations. In the second experiment, a large dataset was used: 605 individuals including 496 ewes (145 Afshari, 54 Shal, 126 Ghezel, and 171 Lori-Bakhtyari sheep), and 109 rams (26 Afshari, 23 Shal, 10 Ghezel, and 50 Lori-Bakhtyari sheep. There were no sheep carrying the G7, G8, or Thoka mutations. Among all 109 rams that were used in this study, none of them were homozygous for the G1 mutation. Moreover, abundance of heterozygote rams (G1/G+) varied from 0.0 (Afshari) to 28.6 % (Lori-Bakhtyari and Ghezel). The highest and the lowest frequencies of the G4 mutation were 30.6 and 3.0 % in Shal and Afshari breeds, respectively. Moreover, G4 abundance varied from 0.0 to 42.3 %, from 3.0 to 26.9, and from 3.0 to 30.6 % in rams, ewes, and overall, respectively. There was a significant difference in the abundance of G1 and G4 mutations between breeds. However, neither the G1 nor the G4 mutation was associated with litter size in Afshari, Ghezel, Lori-Bakhtyari, or Shal sheep breeds. In conclusion, the results of this study showed that GDF9 G1 and G4 mutations are not the reason for higher litter size in Iranian sheep. Moreover, the GDF9 G0 and G6 mutations do not cause triplet births or infertility in Iranian ewes. Therefore, it is unlikely that variant GDF9 mRNA induces larger litter size or infertility in Iranian ewes.

Association Study of Fecundity Gene BMPR1B with Prolificacy in Surti Goats under Farm and Field Condition

2019 ◽  
Vol 14 (04) ◽  
Author(s):  
N. S. Dangar ◽  
G. M. Pandya ◽  
U. V. Ramani ◽  
Y. D. Padheriya ◽  
T. Sangma ◽  
...  
Keyword(s):  
Litter Size ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Pcr Primers ◽  
Dual Purpose ◽  
Protein Receptor ◽  
Morphogenetic Protein ◽  
Pcr Rflp ◽  
Base Size ◽  
Mutation Information

The Surti is a dual purpose goat breed of Gujarat. The bone morphogenetic protein receptor type 1B (BMPR1B) gene of transforming growth factor beta (TGF-β) superfamily ligands is playing a role in ovulation as well as litter size. Mutation in Exon-6 region of BMPR1B gene with base size 190 bp reported increasing litter size. Based on the known mutation information in goat and sheep, PCR primers were designed to screen polymorphism in total 100 Surti goats, 50 Surti goats from University Farm, Navsari and 50 Surti goats from field units of Southern part of Gujarat. During PCR-RFLP study no polymorphic sites were found for Exon-6 region of BMPR1B on Surti goats. Moreover, the twinning rate was 10% in first parity and higher in subsequent second (62.5%) and third (76.8%) parties.

scholarly journals Polymorphism of CAST gene in five sheep breeds in Bulgaria

2021 ◽  
Vol 262 ◽  
pp. 02026
Author(s):  
Ivona Dimitrova ◽  
Milena Bozhilova-Sakova ◽  
Atanaska Teneva ◽  
Tanya Ivanova ◽  
Ina Stoycheva
Keyword(s):  
Genetic Diversity ◽  
Genotypic Variation ◽  
Ovis Aries ◽  
Heterozygous Genotype ◽  
Breeding Programs ◽  
Candidate Marker ◽  
Sheep Breeds ◽  
Homozygous Genotype ◽  
Pcr Rflp ◽  
Cast Gene

CAST gene is a candidate marker that influences the intensity of growth and meat quality. The aim of this study was to investigate the genetic variability of CAST gene in five Bulgarian sheep breeds – two merino, two local and one for milk. A total of 150 ewes, belonging to these breeds were investigated for polymorphisms of CAST gene by using PCR-RFLP method. A 622 bp fragment of Ovis aries CAST gene was amplified using PCR. After restriction with endonuclease MspI two alleles were observed in Ascanian, Caucasian, Breznik and Pleven Blackhead breeds. One allele (M) and one genotype (MM) were detected in Cooper-Red Shumen breed. The highest frequency of the allele N was established in the Ascanian merino breed (0.27) followed by Caucasian merino (0.13), Breznik (0.12) and Pleven Blackhead (0.07). Two genotypes - MM and MN, were observed in Caucasian, Breznik and Pleven Blackhead breeds. The three possible genotypes were found only in Ascanian merino ewes - MM, MN and NN. The lowest frequency of the homozygous genotype MM (0.50) and the highest frequency of the heterozygous genotype MN was established in the Ascanian merino breed (0.47). The obtained results confirm that the PCR-RFLP method can be used to identify different genotypic variation of CAST gene in Bulgarian sheep breeds. The established genetic diversity in the calpastatin gene indicates that, after further associative studies, this gene may be included in the breeding programs of certain sheep breeds.

scholarly journals G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep

2021 ◽  
Vol 14 (1) ◽  
pp. 104-112
Author(s):  
Amani Z. Abdelgadir ◽  
Lutfi M. A. Musa ◽  
Khaleel I. Jawasreh ◽  
Aubai. O. Saleem ◽  
Faisal El-Hag ◽  
...  
Keyword(s):  
Point Mutation ◽  
Litter Size ◽  
Reference Sequence ◽  
Wild Type ◽  
Mutant Type ◽  
Type Allele ◽  
Sheep Breeds ◽  
Differentiation Factor ◽  
Growth Differentiation Factor 9 ◽  
Gdf9 Gene

Background and Aim: Sudanese desert sheep encompass different sheep breeds named according to the different Sudanese tribes that rear them such as the Dubasi, Shugor, and Watish sheep. The objectives of this study were to screen for G1 point mutation in the polymorphic growth differentiation factor 9 (GDF9) gene, investigate its association with litter size, and construct the phylogeny of the different tribal breeds that belong to the Sudanese Desert sheep tribal types. Materials and Methods: Genomic DNA was extracted from whole blood of three tribal Desert sheep breeds (Dubasi, Watish, and Shugor) using the guanidine chloride method. Polymerase chain reaction-restriction fragment length polymorphism with HhaI restriction enzyme and sequencing techniques was used for genotyping the GDF9 locus for possible mutations associated with litter size in the three desert sheep tribal types. Results: G1 mutation in GDF9 caused the replacement of Arginine by Histidine at residue 87. The wild type allele (A) had the highest frequency, whereas the mutant type allele (a) had the lowest in all the sequenced subtypes. The genotype frequencies of the wild type ewes (AA) were higher than the heterozygous (Aa) and the mutant type (aa) frequencies in the three studied desert sheep types. No significant differences were found in the allele frequency between the three tribal types. Litter size was significantly influenced by the genotypes of GDF9 gene, parities, and subtypes (p≤0.01, 0.01, and 0.05, respectively). In the Watish sheep type, heterozygous sheep in their second parity recorded the highest litter size. Sequence alignment of GDF9 gene samples with the database entry indicated that all three tribal types were similar and identical to the reference sequence. The phylogenetic tree revealed that Shugor is the common ancestor of the studied types and Watish is more closely related to Shugor than Dubasi. This result mi ght partly explain the lower reproductive performance of Dubasi compared to Watish and Shugor. Conclusion: The presence of one copy of GDF9 gene increased litter size in the studied Sudanese Desert sheep. This locus may be used as a biomarker for litter size improvement through genotypic selection and allele or gene introgression.

scholarly journals Detection of genetic variations of five MMAF-related genes and their associations with litter size in goat

2019 ◽  
Author(s):  
Zhen Wang ◽  
Yun Pan ◽  
Libang He ◽  
Hong Chen ◽  
Chuanying Pan ◽  
...  
Keyword(s):  
Linkage Disequilibrium ◽  
Litter Size ◽  
Marker Assisted Selection ◽  
The Other ◽  
Sequencing Analysis ◽  
Indel Mutation ◽  
Average Litter Size ◽  
Dna Sequencing Analysis ◽  
Indel Variation ◽  
Selection Of

Abstract Background: Multiple morphological abnormalities of the sperm flagella (MMAF) makes an assignable contribution to male infertility, including QRICH2 , CFAP43 , CFAP44 , CFAP69 , CCDC39 , AKAP4 and DNAH1 gene. This work studied 28 putative indel mutations of MMAF related genes including QRICH2 , CFAP69 , CFAP43 , CCDC39 and DNAH1 gene and their correlation with the first-born litter sizes of 769 Shaanbei white cashmere (SBWC) goats. Results: Electrophoresis and DNA sequencing analysis showed the 11-bp indel within QRICH2 ( QRICH2 -P4), the three indel variations in CFAP69 ( CFAP69 -P4, CFAP69 -P6 and CFAP69 -P7) and the 27-bp indel of DNAH1 ( DNAH1 -P1) were found to be polymorphic. The 27-bp indel variation within DNAH1 was not in consistent with HWE and the other four indel of QRICH2 and CFAP69 were in consistent with HWE. The linkage disequilibrium (LD) analysis showed the 8-bp indel ( CFAP69 -P4) and the 6-bp indel ( CFAP69- P6) within CFAP69 were in complete LD with each other (D'=0.99, r 2 =1.00). The 27-bp indel mutation within DNAH1 was strongly significantly associated with first-born litter sizes of SBWC goats ( P ＜0.01) and the average litter size of II genotype was significantly greater than ID and DD genotypes ( P = 0.003). In single-lamb and multi-lamb of goat groups, the genotype distributions of the 27-bp indel was significantly different ( P = 0.002). While the 11-bp indel variation of QRICH2 and three indel mutations (P4, P6 and P7) of CFAP69 identified were not ( P ＞0.05). Conclusions: These findings suggest the 27-bp indels in the goat DNAH1 can be used as an effective molecular marker for marker-assisted selection of goats reproduction breeding in the future.

MEAT PRODUCTIVITY OF KAZAKH WHITE-HEADED STEERS OF DIFFERENT GENOTYPES BY GENES CAPN1 AND GH

2020 ◽  
Author(s):  
М.И. СЕЛИОНОВА ◽  
В.Р. ПЛАХТЮКОВА
Keyword(s):  
Live Weight ◽  
Average Weight ◽  
Homozygous Genotype ◽  
Significant Difference ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Percent Protein ◽  
Allele Specific ◽  
Meat Productivity ◽  
Selection Of

В статье приводятся результаты исследований полиморфизма генов кальпаина (CAPN1) и гормона роста (GH) и их влияние на динамику живой массы и показатели мясной продуктивности молодняка крупного рогатого скота казахской белоголовой породы. Генотипирование по гену CAPN1 проводилось методом ПЦР-РВ с использованием аллель-специфичных зондов; по гену GH — ПЦР-ПДРФ. Установлено, что частоты встречаемости желательных аллелей С и V, соответственно, в CAPN1 и GH составили 0,40 и 0,12, гомозиготных генотипов — 0,31 и 0,6. Носители генотипов СС и VVв генах CAPN1 и GH имели большую живую массу в возрасте 8 мес на 13,8 и 6,9% и 12 мес на 14,6 и 6,9% по сравнению с GG и LL генотипами. Наибольшие предубойная, убойная масса и масса туши отмечались для туш, полученных от гомозиготных CC и VV бычков в генах CAPN1 и GH. Их превосходство над GG и LL генотипами составило, соответственно, 19,3; 16,2 и 16,4 кг; 19,8; 19,1 и 16,1 кг. Гомозиготные бычки имели лучшие показатели по коэффициенту мясности на 4,1 и 2,0% и убойному выходу — на 1,4 и 2,0%. Содержание белка и жира в длиннейшей мышце спины туш бычков СС и VV-генотипов было выше на 0,28—2,13%. Полученные данные свидетельствуют о перспективности отбора животных, в генотипе которых присутствуют желательные аллели и подбора родительских пар для получения большего числа потомков с гомозиготным генотипом. The results of the investigation on polymorphism of calpain (CAPN1) and growth hormone (GH) genes are presented, and also their influence on live weight dynamics and other traits of meat productivity in Kazakh white-headed young bulls. Genotyping of CAPN1 gene was performed by PCR-RT method using allele-specific probes, GH gene — PCR-RFLP. It was found that frequencies of desired alleles C and V in CAPN1 and GH respectively were 0.40 and 0.12, homozygous genotypes - 0.31 and 0.60. It was determined that CC (CAPN1 gene) and VV (GH gene) genotypes had higher live weight, than GG and LL genotypes. Their exceedance was 13.8 and 6.9% respectively for 8-month age and 14.6 and 6.9% for 12-month age. The highest pre-slaughter, slaughter weight and carcass weight were observed for homozygous CC and VV bulls for CAPN1 and GH genes. Their significant difference over GG and LL genotypes was 19.3, 16.2 and 16.4 kg, 19.8, 19.1 and 16.1 kg respectively. Homozygous CC and VV bulls had average weight of meat, higher by 4.1 and 2.0 percent than bull with other genotypes; and their average slaughter yield was also higher 1.4 and 2.0 abs. percent. Protein and fat content in longissimus dorsi of CC and VV genotypes was 0.28—2.13 abs. higher. The data obtained indicate that selection of the animals whose genotypes contain desirable alleles and matching of parents will increase the frequency of homozygous genotype.

Inverdale fecundity gene (FecXI) influences twin ovulation incidence in pubertal ewe lambs from Texel sires and Cheviot or Scottish Blackface dams

2005 ◽  
Vol 2005 ◽  
pp. 55-55 ◽  
Author(s):  
F. M. Alink ◽  
M. J. A. Mylne ◽  
R. G. Watt ◽  
P. Kenyon ◽  
M.J. Wood ◽  
...  
Keyword(s):  
Litter Size ◽  
Single Gene ◽  
Single Copy ◽  
Female Offspring ◽  
Gene Effects ◽  
Genetic Traits ◽  
Sheep Breeds ◽  
Ewe Lambs ◽  
Homozygous Genotype ◽  
Market Needs

A key to long-term sustainable enhancement of viable livestock production is the introduction of genetic traits that ensure that fertility and meat quality characteristics are compatible with farming environments and market needs. For example, the sheep industry could benefit if daughters of hill-breed ewes were of a crossbred genotype that enhances both carcass characteristics and fertility traits. Use of sires that confer better conformation is an option but does not significantly boost prolificacy. Introduction of the ‘Inverdale’ fecundity gene could change this. On a flock basis in the Romney breed, mean ovulation is increased by 1.0 and litter size by 0.6 in adult ewes carrying a single copy of this gene (designated as FecXI because it is on the X chromosome; Davis et al. 1992). Carrier males transmit it to all of their female offspring, these being heterozygous carriers of the gene unless it also is maternally inherited. In the latter instance, young would be infertile the homozygous genotype confers an undesirable ‘streak ovary’ phenotype. Although a number of sheep breeds world-wide exhibit significant ‘single gene’ effects on ovulation and litter size (Montgomery et al. 2001), Scottish hill sheep breeds show no evidence of this. Consequently, all ewe lambs generated by crossing these hill ewes with a ram carrying the Inverdale gene should be heterozygous. To ascertain whether such animals exhibit enhanced fecundity, an on-farm study investigated ovulation incidence in cyclic ewe lambs born to Cheviot or Scottish Blackface ewes that had been bred to Texel rams carrying a single copy of the ‘Inverdale’ gene.

GDF9 gene polymorphism and its association with litter size in two Russian sheep breeds

2017 ◽  
Vol 29 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Ivan F. Gorlov ◽  
Yuri A. Kolosov ◽  
Nadezhda V. Shirokova ◽  
Lyubov V. Getmantseva ◽  
Marina I. Slozhenkina ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Litter Size ◽  
Sheep Breeds ◽  
Gdf9 Gene

scholarly journals Identifikasi Mutasi FecX Pada Gen BMP15 dan Pengaruhnya Terhadap Sifat Prolifik pada Kambing Lokal di Kabupaten Lombok Barat

2019 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Rahmat Agus Hidayat ◽  
Sulaiman Ngongu Depamede ◽  
Maskur Maskur
Keyword(s):  
Litter Size ◽  
Mutant Allele ◽  
Secondary Data ◽  
Wild Type ◽  
Banding Patterns ◽  
Average Litter Size ◽  
Pcr Rflp ◽  
Pcr Products ◽  
Visually Based ◽  
Its Polymorphism

The aims of this study were to identify the mutations of FecX gene in the local goats and to analyze its polymorphism as well as its influence on the prolific nature of the local goats in West Lombok Regency, Indonesia. The study was conducted in the Immunobiology Laboratory of Mataram University, using DNA isolated from 100 blood samples of local female goats which have given birth of once to three times. The methods used were PCR-RFLP method and the PCR products were digested with HinfI restriction enzyme (G|ANTC) then analyzed visually based on DNA banding patterns on 2% agarose gels. The frequency of allele and genotype obtained, were then analyzed through a comparison with the secondary data of litter size obtained from the local goat keepers information. The results showed that the gene mutation of FecXG produced two alleles: "wild-type" (+) sized of 110 bp and 31 bp, and the mutant allele (G) of 141 bp with the allele frequency of 0,965 and 0,035 respectively. Combinations of alleles in the gene BMP15 produced two genotypes, namely (a) genotype ++ (110 bp/110 bp) with a frequency of 0.93, with the average litter size of 1.59 ± 0.319, and (b) genotype G + (141bp/110 bp), with a frequency of 0.07 and with the average litter size of 1.65 ± 0.202. The results of this study indicated that mutation occurred in BMP15 gene, i.e. FecXG gene, the gene responsible for the prolificacy of animals studied. Furthermore there was a correlation between polymorphism of FecXG gene and the prolific nature of the local goats, which was predicted to lead the divergence in litter size of each local goat genotype  

scholarly journals Polymorphism of FABP3 gene and its effect on litter size and milk production of synthetic population bulgarian milk ewes

2021 ◽  
Vol 28 ◽  
pp. 48-52
Author(s):  
I. V. Dimitrova ◽  
M. G. Bozhilova-Sakova ◽  
T. S. Ivanova ◽  
V. I. Koutev ◽  
M. M. Ignatova
Keyword(s):  
Milk Production ◽  
Litter Size ◽  
Fatty Acid Binding Protein ◽  
Protein Gene ◽  
Animal Science ◽  
Fatty Acid Binding ◽  
Homozygous Genotype ◽  
Pcr Rflp ◽  
Synthetic Population ◽  
Binding Protein Gene

Aim. The purpose of this work was to study polymorphism of FABP3 (heart-type fatty acid binding protein) gene and its effect on litter size and milk production. The experiment included 30 ewes of Synthetic Population Bulgarian Milk breed from Institute of Animal Science – Kostinbrod. Methods. By PCR-RFLP method with endonuclease BseDI in SNP3 of FABP3 gene were detected two genotypes – GG and AG. Results. In SNP3 of FABP3 gene the frequency of allele G was 0.85 and the frequency of allele A was 0.15. The genotype GG was with frequency 0.70 and AG – with 0.30. Conclusions. In this study of ewes from SPBM breed, the presence of heterozygous genotype AG in SNP3 of the FABP3 was associated with increased litter size, while the presence of the homozygous genotype GG led to increased milk productivity during lactation. Keywords: sheep, FABP3 gene, litter size, milk production.

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