In situ Cu2+-triggered wavelength-tunable fluorescent sensor for highly sensitive sensing inorganic pyrophosphatase activity and its logic gate application

2021 ◽  
pp. 130439
Author(s):  
Can Chen ◽  
Guotong Ruan ◽  
Yujie Sun ◽  
Lili Wang ◽  
Chengwu Zhang ◽  
...  
Keyword(s):  
Fluorescent Sensor ◽  
Logic Gate ◽  
Inorganic Pyrophosphatase ◽  
Wavelength Tunable ◽  
Highly Sensitive ◽  
Pyrophosphatase Activity

Rational design of a highly sensitive and selective “turn-on” fluorescent sensor for PO43− detection

2015 ◽  
Vol 44 (48) ◽  
pp. 20830-20833 ◽  
Author(s):  
Si-Quan Jiang ◽  
Zi-Yan Zhou ◽  
Shu-Ping Zhuo ◽  
Guo-Gang Shan ◽  
Ling-Bao Xing ◽  
...  
Keyword(s):  
Schiff Base ◽  
Rational Design ◽  
Fluorescent Sensor ◽  
Turn On ◽  
Base Unit ◽  
Highly Sensitive

An in situ-generated iron(iii) complex with a 1,8-naphthalene-based Schiff base unit has been rationally designed, which exhibits a highly selective response and excellent sensitivity for the turn-on detection of PO43− anions.

Real-time assay of inorganic pyrophosphatase activity in the red region based on Li+-doped NaYF4:Yb,Er upconversion luminescent nanoparticles

2017 ◽  
Vol 9 (22) ◽  
pp. 3296-3301 ◽  
Author(s):  
Lei Wang ◽  
Yuan Ji ◽  
Yuezhen He ◽  
Lun Wang ◽  
Hongqi Chen
Keyword(s):  
Real Time ◽  
Inorganic Pyrophosphatase ◽  
Competition Assay ◽  
Highly Sensitive ◽  
Luminescent Nanoparticles ◽  
Pyrophosphatase Activity ◽  
Real Time Detection ◽  
Sensitive Method

Based on a competition assay approach, a new highly sensitive method for real-time detection of PPase activity using Li+-doped NaYF4:Yb,Er upconversion red luminescent nanoparticles was developed.

scholarly journals A highly sensitive and selective on–off fluorescent sensor based on a complex of polySchiff-Fe2+ for Cr(vi) detection in an aqueous medium

RSC Advances ◽  
2021 ◽  
Vol 11 (15) ◽  
pp. 8963-8969
Author(s):  
Zhiying Li ◽  
Congshu Li ◽  
Huiyun Yan
Keyword(s):  
Aqueous Medium ◽  
Fluorescent Sensor ◽  
Human Race ◽  
Safety And Health ◽  
Highly Sensitive

Designing exceptional probes to detect minute quantities of chromium(vi) is of huge importance for the safety and health of the human race.

scholarly journals Spatial expression pattern of serine proteases in the blood fluke Schistosoma mansoni determined by fluorescence RNA in situ hybridization

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Lenka Ulrychová ◽  
Pavel Ostašov ◽  
Marta Chanová ◽  
Michael Mareš ◽  
Martin Horn ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Schistosoma Mansoni ◽  
Rna Sequencing ◽  
Serine Proteases ◽  
Expression Patterns ◽  
High Sensitivity ◽  
Host Parasite Interactions ◽  
Highly Sensitive ◽  
Host Parasite

Abstract Background The blood flukes of genus Schistosoma are the causative agent of schistosomiasis, a parasitic disease that infects more than 200 million people worldwide. Proteases of schistosomes are involved in critical steps of host–parasite interactions and are promising therapeutic targets. We recently identified and characterized a group of S1 family Schistosoma mansoni serine proteases, including SmSP1 to SmSP5. Expression levels of some SmSPs in S. mansoni are low, and by standard genome sequencing technologies they are marginally detectable at the method threshold levels. Here, we report their spatial gene expression patterns in adult S. mansoni by the high-sensitivity localization assay. Methodology Highly sensitive fluorescence in situ RNA hybridization (FISH) was modified and used for the localization of mRNAs encoding individual SmSP proteases (including low-expressed SmSPs) in tissues of adult worms. High sensitivity was obtained due to specifically prepared tissue and probes in combination with the employment of a signal amplification approach. The assay method was validated by detecting the expression patterns of a set of relevant reference genes including SmCB1, SmPOP, SmTSP-2, and Sm29 with localization formerly determined by other techniques. Results FISH analysis revealed interesting expression patterns of SmSPs distributed in multiple tissues of S. mansoni adults. The expression patterns of individual SmSPs were distinct but in part overlapping and were consistent with existing transcriptome sequencing data. The exception were genes with significantly low expression, which were also localized in tissues where they had not previously been detected by RNA sequencing methods. In general, SmSPs were found in various tissues including reproductive organs, parenchymal cells, esophagus, and the tegumental surface. Conclusions The FISH-based assay provided spatial information about the expression of five SmSPs in adult S. mansoni females and males. This highly sensitive method allowed visualization of low-abundantly expressed genes that are below the detection limits of standard in situ hybridization or by RNA sequencing. Thus, this technical approach turned out to be suitable for sensitive localization studies and may also be applicable for other trematodes. The results suggest that SmSPs may play roles in diverse processes of the parasite. Certain SmSPs expressed at the surface may be involved in host–parasite interactions. Graphic abstract

Sign in / Sign up

Export Citation Format

Share Document