In Situ and Intraoperative Detection of the Ureter Injury Using a Highly Sensitive Piezoresistive Sensor with a Tunable Porous Structure

2021 ◽  
Author(s):  
Shan Wang ◽  
Guorui Chen ◽  
Bing Yao ◽  
Adrian J. Y. Chee ◽  
Zongrong Wang ◽  
...  
Keyword(s):  
Porous Structure ◽  
Ureter Injury ◽  
Intraoperative Detection ◽  
Piezoresistive Sensor ◽  
Highly Sensitive

scholarly journals Spatial expression pattern of serine proteases in the blood fluke Schistosoma mansoni determined by fluorescence RNA in situ hybridization

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Lenka Ulrychová ◽  
Pavel Ostašov ◽  
Marta Chanová ◽  
Michael Mareš ◽  
Martin Horn ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Schistosoma Mansoni ◽  
Rna Sequencing ◽  
Serine Proteases ◽  
Expression Patterns ◽  
High Sensitivity ◽  
Host Parasite Interactions ◽  
Highly Sensitive ◽  
Host Parasite

Abstract Background The blood flukes of genus Schistosoma are the causative agent of schistosomiasis, a parasitic disease that infects more than 200 million people worldwide. Proteases of schistosomes are involved in critical steps of host–parasite interactions and are promising therapeutic targets. We recently identified and characterized a group of S1 family Schistosoma mansoni serine proteases, including SmSP1 to SmSP5. Expression levels of some SmSPs in S. mansoni are low, and by standard genome sequencing technologies they are marginally detectable at the method threshold levels. Here, we report their spatial gene expression patterns in adult S. mansoni by the high-sensitivity localization assay. Methodology Highly sensitive fluorescence in situ RNA hybridization (FISH) was modified and used for the localization of mRNAs encoding individual SmSP proteases (including low-expressed SmSPs) in tissues of adult worms. High sensitivity was obtained due to specifically prepared tissue and probes in combination with the employment of a signal amplification approach. The assay method was validated by detecting the expression patterns of a set of relevant reference genes including SmCB1, SmPOP, SmTSP-2, and Sm29 with localization formerly determined by other techniques. Results FISH analysis revealed interesting expression patterns of SmSPs distributed in multiple tissues of S. mansoni adults. The expression patterns of individual SmSPs were distinct but in part overlapping and were consistent with existing transcriptome sequencing data. The exception were genes with significantly low expression, which were also localized in tissues where they had not previously been detected by RNA sequencing methods. In general, SmSPs were found in various tissues including reproductive organs, parenchymal cells, esophagus, and the tegumental surface. Conclusions The FISH-based assay provided spatial information about the expression of five SmSPs in adult S. mansoni females and males. This highly sensitive method allowed visualization of low-abundantly expressed genes that are below the detection limits of standard in situ hybridization or by RNA sequencing. Thus, this technical approach turned out to be suitable for sensitive localization studies and may also be applicable for other trematodes. The results suggest that SmSPs may play roles in diverse processes of the parasite. Certain SmSPs expressed at the surface may be involved in host–parasite interactions. Graphic abstract

Highly Sensitive Piezoresistive Sensor Based on Modified Polystyrene Microsphere Multi-layer Stacking Conductive Interface

2019 ◽  
Vol 48 (10) ◽  
pp. 1206-1208 ◽  
Author(s):  
Liuyi Ding ◽  
Long Zhang ◽  
Shaohui Zhang ◽  
Chao Wang ◽  
Ziyang Chen ◽  
...  
Keyword(s):  
Polystyrene Microsphere ◽  
Piezoresistive Sensor ◽  
Highly Sensitive ◽  
Layer Stacking ◽  
Modified Polystyrene

A highly sensitive chlorine gas sensor and enhanced thermal DC electrical conductivity from polypyrrole/silicon carbide nanocomposites

RSC Advances ◽  
2016 ◽  
Vol 6 (87) ◽  
pp. 84200-84208 ◽  
Author(s):  
Adil Sultan ◽  
Sharique Ahmad ◽  
Faiz Mohammad
Keyword(s):  
Electrical Conductivity ◽  
Silicon Carbide ◽  
Gas Sensor ◽  
Dodecylbenzenesulfonic Acid ◽  
Chemical Polymerization ◽  
Dc Electrical Conductivity ◽  
Highly Sensitive ◽  
Chlorine Gas

We report the synthesis of polypyrrole (PPy) and polypyrrole/silicon carbide nanocomposites (PPy/SiC) and PPy/SiC/dodecylbenzenesulfonic acid (DBSA) by in situ chemical polymerization and their application as sensors for the detection of highly toxic chlorine gas.

Wood-Polymer Composite Prepared by In Situ Synthesis of Copolymer within Wood and its Dimensional Stability

2010 ◽  
Vol 150-151 ◽  
pp. 1-5
Author(s):  
Yong Feng Li ◽  
Chi Jiang ◽  
Duo Jun Lv ◽  
Xiao Ying Dong ◽  
Yi Xing Liu
Keyword(s):  
Contact Angle ◽  
Porous Structure ◽  
Polymer Composite ◽  
Dimensional Stability ◽  
Value Added ◽  
Hydroxyl Groups ◽  
Wood Polymer ◽  
Volume Swelling ◽  
Wood Polymer Composite

In order to improve the value-added applications of low-quality wood, a novel Wood-Polymer Composite was fabricated by in-situ synthesis of copolymer from monomers within wood porous structure. The structure was characterized with SEM and FTIR, and its dimensional stability was also tested. The SEM observations showed that copolymer filled up wood pores and contact tightly with wood matrix, indicating strong interactions between them. FTIR analysis indicated that when the monomers copolymerized in situ wood porous structure, they also reacted with wood matrix by reaction of hydroxyl groups and ester groups, indicating chemical bond between the two phases, which is agreement with SEM observations. The volume swelling efficiency and contact angle of such composite were higher than those of wood, respectively, indicating good dimensional stability involving volume swelling efficiency and contact angle. Such composite could be potentially applied in fields of construction, traffic and indoor decoration.

Performance of Wood-Polymer Composite Prepared by In Situ Synthesis of Terpolymer within Wood

2010 ◽  
Vol 34-35 ◽  
pp. 1165-1169 ◽  
Author(s):  
Yong Feng Li ◽  
Bao Gang Wang ◽  
Qi Liang Fu ◽  
Yi Xing Liu ◽  
Xiao Ying Dong
Keyword(s):  
Mechanical Properties ◽  
Porous Structure ◽  
Polymer Composite ◽  
Value Added ◽  
Untreated Wood ◽  
Modulus Of Rupture ◽  
Hydroxyl Group ◽  
Wood Polymer ◽  
Wood Polymer Composite

In order to improve the value-added applications of low-quality wood, a novel composite, wood-polymer composite, was fabricated by in-situ terpolymerization of MMA, VAc and St within wood porous structure. The structure of the composite and the reaction of monomers within wood were both analyzed by SEM and FTIR, and the mechanical properties were also evaluated. The SEM observation showed that the polymer mainly filled up wood pores, suggesting good polymerizating crafts. The FTIR results indicated that under the employed crafts, three monomers terpolymerized in wood porous structure, and grafted onto wood matrix through reaction of ester group from monomers and hydroxyl group from wood components, suggesting chemical combination between the two phases. The mechanical properties of the wood-polymer composite involving modulus of rupture, compressive strength, wearability and hardness were improved 69%, 68%, 36% and 210% over those of untreated wood, respectively. Such method seems to be an effective way to converting low-quality wood to high-quality wood.

scholarly journals A Flexible, Highly Sensitive, and Selective Chemiresistive Gas Sensor Obtained by In Situ Photopolymerization of an Acrylic Resin in the Presence of MWCNTs

2018 ◽  
Vol 304 (2) ◽  
pp. 1800453 ◽  
Author(s):  
Lorenzo Vigna ◽  
Andrea Fasoli ◽  
Matteo Cocuzza ◽  
Fabrizio C. Pirri ◽  
Luisa D. Bozano ◽  
...  
Keyword(s):  
Gas Sensor ◽  
Acrylic Resin ◽  
Highly Sensitive
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