Determination of catechins and caffeine in proposed green tea standard reference materials by liquid chromatography-particle beam/electron ionization mass spectrometry (LC-PB/EIMS)

Talanta ◽  
2010 ◽  
Vol 82 (5) ◽  
pp. 1687-1695 ◽  
Author(s):  
Joaudimir Castro ◽  
Tara Pregibon ◽  
Kristina Chumanov ◽  
R. Kenneth Marcus
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Green Tea ◽  
Reference Materials ◽  
Particle Beam ◽  
Electron Ionization Mass Spectrometry ◽  
Standard Reference Materials ◽  
Ionization Mass ◽  
Beam Electron

scholarly journals Liquid Chromatography-Particle Beam Electron Ionization Mass Spectrometry Method for Analysis of Botanical Extracts: Evaluation of Ephedrine Alkaloids in Standard Reference Materials

2010 ◽  
Vol 93 (6) ◽  
pp. 1788-1797 ◽  
Author(s):  
Joaudimir Castro ◽  
M V Balarama Krishna ◽  
R Kenneth Marcus
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Reference Materials ◽  
Particle Beam ◽  
Standard Reference Materials ◽  
Spectrometry Method ◽  
Mass Spectrometry Method ◽  
Ephedra Sinica ◽  
Botanical Extracts ◽  
Ephedra Sinica Stapf

Abstract The preliminary validation of a high-performance liquid chromatography particle beam mass spectrometry method (HPLC-PB/MS) with electron impact ionization source for analysis of botanical extracts is presented. The LC-PB/MS system was evaluated for the analysis of ephedrine alkaloids using ephedra-containing National Institute of Standards and Technology dietary supplement standard reference materials (SRMs) 3241 Ephedra Sinica Stapf Native Extract and 3242 Ephedra Sinica Stapf Commercial Extract. The ephedrine alkaloids were separated by reversed-phase chromatography using a phenyl column at room temperature. A linear gradient method with a mobile phase composition varying from 5:95 [MeOH:0.1 trifluoroacetic acid (TFA) in water] to 20:80 (MeOH:0.1 TFA in water) at a flow rate of 1.0 mL/min, with an analysis time of less than 20 min, was used. The source block temperature was evaluated to determine the optimal operating conditions by monitoring the intensities and fragmentation patterns of the ephedrine alkaloids. Ephedrine and N-methylephedrine were taken as a representative of the test alkaloids. The LODs on the sub-nanogram level were achieved, with ephedrine, pseudoephedrine, and methylephedrine in the SRMs quantified by a standard addition method with recoveries of 86 and RSDs of 14 (N = 3).

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