Determination of metabolites in Uncaria sinensis by HPLC and GC–MS after green solvent microwave-assisted extraction

This research was conducted to determine the influence of the use of the choline chloride-sorbitol based microwave assisted extraction (MAE) method against the total extraction of polyphenols from the leaf Kadamba (Mitragyna speciosa Korth). Simplisia extracted using solvent (green solvent) choline chloride-sorbitol with a ratio of 1:1; 1:2 and 1:3 g/g with sample and solvent ratio of 1:10 g/mL, microwave strength is 50% watt and performed for 10 minutes, the extract solution has been obtained and then done determination of total levels of polyphenols by using folin ciocalteu reagents and further measured its absorption with the UV-VIS spectrophotometer device at a wavelength of 770 nm. Based on the results of the study, obtained the levels of polyphenols from each comparison of solvent used against the sample of 334 mg of GAE/g samples (1:1 g/g solvent); 372 mg GAE/g samples (1:2 g/g solvents) and 299 mg of GAE/g samples (1:3 g/g solvents). Based on the results of the study can be concluded that the method of choline chloride-sorbitol based microwave assisted extraction (MAE) can be used to extract the polyphenols from Kadamba leaves easily, quickly and efficiently.

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Ekstraksi Polifenol Total dari Umbi Bawang Dayak (Eleutherine bulbosa [Mill.] Urb.) Menggunakan Metode Citric Acid-Glucose based Microwave Assisted Extraction

Proceeding of Mulawarman Pharmaceuticals Conferences ◽

10.25026/mpc.v10i1.379 ◽

2019 ◽

Vol 10 ◽

pp. 139-142

Author(s):

Selvy Jumiatul Astati ◽

Mirhansyah Ardana ◽

Islamudin Ahmad

Keyword(s):

Citric Acid ◽

Environmentally Friendly ◽

Solvent Ratio ◽

Microwave Assisted Extraction ◽

Green Solvent ◽

Microwave Assisted ◽

Onion Bulbs ◽

Assisted Extraction ◽

Total Extraction

The use of environmentally friendly solvent-based expulsion methods today began to be widely used, one of which is a microwave-assisted extraction (MAE) using a citric acid-glucose acid solvent. The study aims to determine the influence of the use of citric acid-glucose based microwave-assisted extraction against the total extraction of polyphenols from Dayak onion bulbs (Eleutherine bulbosa [Mill.] Urb.).The dried samples were extracted using a solvent (green solvent) citric acid – glucose with a ratio of 4:1, 5:1 and 6:1 g/g with the extraction conditions in the form of microwave strength of 50% for 10 minutes with a sample and solvent ratio of 1:10 g/mL, then Extract solution obtained further determination of the total levels of polyphenols using Folin ciocalteu reagents, and its absorption was measured using a UV-vis spectrophotometer at a wavelength of 791 nm. Based on the results of the study, the total amount of polyphenols obtained from each of the solvent conditions used against the samples was 21.92 mgGAE/g samples, 13.86 mgGAE/g samples, and 14.38 mgGAE/g samples. From this study, it can be concluded that the concentration of citric acid in the microwave-based citric acid-glucose method is assisted by extraction according to the ability of the solvent in the competency of polyphenols from Dayak onion bulbs.

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