Flavonoids electrochemical detection in fruit extracts and total antioxidant capacity evaluation

Talanta ◽  
2016 ◽  
Vol 154 ◽  
pp. 284-291 ◽  
Author(s):  
Sara M.C. Gomes ◽  
Mariana-Emilia Ghica ◽  
Isaide Araujo Rodrigues ◽  
Eric de Souza Gil ◽  
Ana Maria Oliveira-Brett
Keyword(s):  
Antioxidant Capacity ◽  
Electrochemical Detection ◽  
Total Antioxidant Capacity ◽  
Fruit Extracts ◽  
Capacity Evaluation ◽  
Total Antioxidant

Flavonoids in Selected Mediterranean Fruits: Extraction, Electrochemical Detection and Total Antioxidant Capacity Evaluation

2016 ◽  
Vol 29 (2) ◽  
pp. 358-366 ◽  
Author(s):  
Fatima Zohra Issaad ◽  
Isabel P. G. Fernandes ◽  
Teodor A. Enache ◽  
Chabane Mouats ◽  
Isaide A. Rodrigues ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Electrochemical Detection ◽  
Total Antioxidant Capacity ◽  
Capacity Evaluation ◽  
Total Antioxidant

Total antioxidant capacity evaluation: Critical steps for assaying berry antioxidant features

BioFactors ◽  
2005 ◽  
Vol 23 (4) ◽  
pp. 221-227 ◽  
Author(s):  
Jessica Scalzo ◽  
Bruno Mezzetti ◽  
Maurizio Battino
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Capacity Evaluation ◽  
Total Antioxidant

Calligonum polygonoides Linnaeus Extract: HPLC‐EC and Total Antioxidant Capacity Evaluation

2015 ◽  
Vol 27 (2) ◽  
pp. 293-301 ◽  
Author(s):  
Sara M. C. Gomes ◽  
Isabel P. G. Fernandes ◽  
Narpat Singh Shekhawat ◽  
Sunita Kumbhat ◽  
Ana Maria Oliveira‐Brett
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Capacity Evaluation ◽  
Total Antioxidant

Phenolic content and antioxidant activity ofZiziphus jujubaMill. fruit extracts

2018 ◽  
Vol 17 (2) ◽  
pp. 74-82
Author(s):  
S. Adjdir ◽  
N. Benariba ◽  
H. Laoufi ◽  
R. Djaziri
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Gallic Acid ◽  
Total Antioxidant Capacity ◽  
Butylated Hydroxytoluene ◽  
Antioxidant Power ◽  
Fruit Extracts ◽  
Total Antioxidant ◽  
Dried Fruit ◽  
Β Carotene

The aim of this study is to evaluate the antioxidant activity of dried fruit extracts of Zizyphus jujubaMill. Polyphenols and flavonoids content determined in aqueous extract (aq), hydromethanolic (EM), hydroacetonic (EA) extracts, and their ethyl acetate (Acet) andn-butanol (n-but) fractions reveal variable rates; 42.84–94.70 mg gallic acid equivalent per g extract and 47.02–427.33 mg catechin equivalent per g extract. The antioxidant activity was evaluated by different assays using total antioxidant capacity, 2,2-Diphenyl-1-picrylhydrazyl (DPPH), β-carotene bleaching assay, and ferric reducing antioxidant power (FRAP). In DPPH assay, all extracts showed an interesting scavenging effect, an estimated reduction rate about 75.58–97.71% was determined with the IC50(the half maximal inhibitory concentration) values ranging from 12 to 503.6 μg/ml. In β-carotene bleaching assay, 500 μg/ml of each extract showed a large inhibition (51.14–85.01%) with IC50values ranging from 48 to 462 μg/ml compared to butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), and tannic acid 0.6, 2.00, and 461 μg/ml, respectively. The results of FRAP show that the crude extracts (aq ˃ EM ˃ EA) are less efficient than their fractions (Acet/aq˃ Acet/EA˃n-but/EA) which demonstrate an important activity. Concerning total antioxidant capacity essay (TAC), the highest capacity was noticed for Acet/aq (71.98 μg gallic acid equivalents [GAE]/mg extract) and Acet/EA extract (64.62 μg GAE/mg of extract). The antioxidant activity of dried fruit extracts ofZ. jujubais mainly related to its polyphenol content essentially on flavonoids, which qualifies this species as a source of natural antioxidant.

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

Summer Day/Night Gender Differences in Serum Total Antioxidant Capacity as a Methodological Pitfall in Human Antioxidant Research

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Armando L. Morera-Fumero ◽  
Pedro Abreu-Gonzalez ◽  
Lourdes Fernandez- Lopez ◽  
Estefanía Díaz-Mesa ◽  
Maria del Rosario Cejas-Mendez
Keyword(s):  
Gender Differences ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Total Antioxidant

EXTRACTION OPTIMIZATION AND ANTIOXIDANT ACTIVITY OF PHENOLIC COMPOUNDS FROM AVOCADO PEEL

2019 ◽  
pp. 49-59
Author(s):  
Nu Linh Giang Ton ◽  
Thi Hoai Nguyen ◽  
Quoc Hung Vo
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Antioxidant Capacity ◽  
Response Surface ◽  
Total Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Quadratic Model ◽  
Total Phenolic ◽  
Total Antioxidant

Avocado peel has been considered as a potential source of natural antioxidants in which phenolics are among the most important compounds. Therefore, this study aims to optimize the extraction process of phenolics using response surface methodology and evaluate the corresponding antioxidant activity. From the quadratic model, the optimal condition was determined including the ethanol concentration 54.55% (v/v), the solvent/solute ratio 71.82/1 (mL/g), temperature 53.03 oC and extraction time 99.09 min. The total phenolic content and the total antioxidant capacity at this condition with minor modifications were 26,74 ± 0,04 (mg GAE/g DW) and 188.06 ± 1.41 (mg AAE/g DW), respectively. The significant correlation between total phenolic content and total antioxidant capacity was also confirmed. Key words: response surface methodology, central composite rotatable design, total phenolic content, total antioxidant capacity, avocado peel

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