High-efficiency artificial enzyme cascade bio-platform based on MOF-derived bimetal nanocomposite for biosensing

Talanta ◽  
2020 ◽  
Vol 220 ◽  
pp. 121374 ◽  
Author(s):  
Min Liu ◽  
Junsong Mou ◽  
Xiaohan Xu ◽  
Feifei Zhang ◽  
Jianfei Xia ◽  
...  
The Analyst ◽  
2022 ◽  
Author(s):  
Leilei Meng ◽  
Rui Hu ◽  
Jinping Chen ◽  
Tianjun Yu ◽  
Xiaopin Cai ◽  
...  

Quantification of phenylalanine in clinic samples is essential to diagnosis and treatment of phenylketonuria in neonates. In this report, an enzyme cascade strategy was proposed and a high efficiency fluorescence...


2019 ◽  
Vol 41 (4-5) ◽  
pp. 605-611 ◽  
Author(s):  
Takenori Satomura ◽  
Kousaku Horinaga ◽  
Shino Tanaka ◽  
Eiichiro Takamura ◽  
Hiroaki Sakamoto ◽  
...  

2015 ◽  
Vol 71 (12) ◽  
pp. 1475-1480
Author(s):  
Iuliia Iermak ◽  
Oksana Degtjarik ◽  
Fabian Steffler ◽  
Volker Sieber ◽  
Ivana Kuta Smatanova

The glyceraldehyde dehydrogenase fromThermoplasma acidophilum(TaAlDH) is a microbial enzyme that catalyzes the oxidation of D-glyceraldehyde to D-glycerate in the artificial enzyme cascade designed for the conversion of glucose to the organic solvents isobutanol and ethanol. Various mutants ofTaAlDH were constructed by a random approach followed by site-directed and saturation mutagenesis in order to improve the properties of the enzyme that are essential for its functioning within the cascade. Two enzyme variants, wild-typeTaAlDH (TaAlDHwt) and an F34M+S405N variant (TaAlDH F34M+S405N), were successfully crystallized. Crystals ofTaAlDHwt belonged to the monoclinic space groupP1211 with eight molecules per asymmetric unit and diffracted to a resolution of 1.95 Å.TaAlDH F34M+S405N crystallized in two different space groups: triclinicP1 with 16 molecules per asymmetric unit and monoclinicC121 with four molecules per asymmetric unit. These crystals diffracted to resolutions of 2.14 and 2.10 Å for theP1 andC121 crystals, respectively.


2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Benedict Ryan Lukito ◽  
Zilong Wang ◽  
Balaji Sundara Sekar ◽  
Zhi Li

Abstract(R)-mandelic acid is an industrially important chemical, especially used for producing antibiotics. Its chemical synthesis often uses highly toxic cyanide to produce its racemic form, followed by kinetic resolution with 50% maximum yield. Here we report a green and sustainable biocatalytic method for producing (R)-mandelic acid from easily available styrene, biobased L-phenylalanine, and renewable feedstocks such as glycerol and glucose, respectively. An epoxidation-hydrolysis-double oxidation artificial enzyme cascade was developed to produce (R)-mandelic acid at 1.52 g/L from styrene with > 99% ee. Incorporation of deamination and decarboxylation into the above cascade enables direct conversion of L-phenylalanine to (R)-mandelic acid at 913 mg/L and > 99% ee. Expressing the five-enzyme cascade in an L-phenylalanine-overproducing E. coli NST74 strain led to the direct synthesis of (R)-mandelic acid from glycerol or glucose, affording 228 or 152 mg/L product via fermentation. Moreover, coupling of E. coli cells expressing L-phenylalanine biosynthesis pathway with E. coli cells expressing the artificial enzyme cascade enabled the production of 760 or 455 mg/L (R)-mandelic acid from glycerol or glucose. These simple, safe, and green methods show great potential in producing (R)-mandelic acid from renewable feedstocks.


2014 ◽  
Vol 31 ◽  
pp. S75
Author(s):  
Wolfgang Kroutil ◽  
Johann Sattler ◽  
Michael Fuchs ◽  
Verena Resch ◽  
Joerg Schrittwieser

ChemCatChem ◽  
2015 ◽  
Vol 7 (23) ◽  
pp. 3951-3955 ◽  
Author(s):  
Sandy Schmidt ◽  
Hanna C. Büchsenschütz ◽  
Christian Scherkus ◽  
Andreas Liese ◽  
Harald Gröger ◽  
...  

The Analyst ◽  
2021 ◽  
Author(s):  
Huiying Xu ◽  
Lu Zheng ◽  
Yu Zhou ◽  
Bang-Ce Ye

Tumor-related exosomes, which are heterogeneous membrane-enclosed nanovesicles shed from cancer cells, have been widely recognized as potential noninvasive biomarkers for early cancer diagnosis. Herein, an artificial enzyme cascade amplification strategy...


1988 ◽  
Vol 102 ◽  
pp. 41
Author(s):  
E. Silver ◽  
C. Hailey ◽  
S. Labov ◽  
N. Madden ◽  
D. Landis ◽  
...  

The merits of microcalorimetry below 1°K for high resolution spectroscopy has become widely recognized on theoretical grounds. By combining the high efficiency, broadband spectral sensitivity of traditional photoelectric detectors with the high resolution capabilities characteristic of dispersive spectrometers, the microcalorimeter could potentially revolutionize spectroscopic measurements of astrophysical and laboratory plasmas. In actuality, however, the performance of prototype instruments has fallen short of theoretical predictions and practical detectors are still unavailable for use as laboratory and space-based instruments. These issues are currently being addressed by the new collaborative initiative between LLNL, LBL, U.C.I., U.C.B., and U.C.D.. Microcalorimeters of various types are being developed and tested at temperatures of 1.4, 0.3, and 0.1°K. These include monolithic devices made from NTD Germanium and composite configurations using sapphire substrates with temperature sensors fabricated from NTD Germanium, evaporative films of Germanium-Gold alloy, or material with superconducting transition edges. A new approache to low noise pulse counting electronics has been developed that allows the ultimate speed of the device to be determined solely by the detector thermal response and geometry. Our laboratory studies of the thermal and resistive properties of these and other candidate materials should enable us to characterize the pulse shape and subsequently predict the ultimate performance. We are building a compact adiabatic demagnetization refrigerator for conveniently reaching 0.1°K in the laboratory and for use in future satellite-borne missions. A description of this instrument together with results from our most recent experiments will be presented.


Author(s):  
J. M. Cowley ◽  
R. Glaisher ◽  
J. A. Lin ◽  
H.-J. Ou

Some of the most important applications of STEM depend on the variety of imaging and diffraction made possible by the versatility of the detector system and the serial nature, of the image acquisition. A special detector system, previously described, has been added to our STEM instrument to allow us to take full advantage of this versatility. In this, the diffraction pattern in the detector plane may be formed on either of two phosphor screens, one with P47 (very fast) phosphor and the other with P20 (high efficiency) phosphor. The light from the phosphor is conveyed through a fiber-optic rod to an image intensifier and TV system and may be photographed, recorded on videotape, or stored digitally on a frame store. The P47 screen has a hole through it to allow electrons to enter a Gatan EELS spectrometer. Recently a modified SEM detector has been added so that high resolution (10Å) imaging with secondary electrons may be used in conjunction with other modes.


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