scholarly journals A proof-of-concept study utilising 2D NMR spectrometry for in situ characterisation and quantitation of key biomarkers and actives in tape stripped ex vivo human skin

Talanta ◽  
2021 ◽  
pp. 122980
Author(s):  
Cameron Robertson ◽  
Nidhin Raj ◽  
Robert Lucas ◽  
Tomris Coban ◽  
Adam Le Gresley
2019 ◽  
Vol 58 (1) ◽  
pp. 100-106
Author(s):  
Suzanne R. Thibodeaux ◽  
Yvette C. Tanhehco ◽  
Leah Irwin ◽  
Lita Jamensky ◽  
Kevin Schell ◽  
...  

BMJ Open ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. e028572
Author(s):  
Amy Halls ◽  
Mohan Kanagasundaram ◽  
Margaret Lau-Walker ◽  
Hilary Diack ◽  
Simon Bettles

ObjectiveAcutely unwell patients in the primary care setting are uncommon, but their successful management requires involvement from staff (clinical and non-clinical) working as a cohesive team. Despite the advantages of interprofessional education being well documented, there is little research evidence of this within primary care. Enhancing interprofessional working could ultimately improve care of the acutely ill patient. This proof of concept study aimed to develop an in situ simulation of a medical emergency to use within primary care, and assess its acceptability and utility through participants’ reported experiences.SettingThree research-active General Practices in south east England. Nine staff members per practice consented to participate, representing clinical and non-clinical professions.MethodsThe intervention of an in situ simulation scenario of a cardiac arrest was developed by the research team. For the evaluation, staff participated in individual qualitative semistructured interviews following the in situ simulation: these focused on their experiences of participating, with particular attention on interdisciplinary training and potential future developments of the in situ simulation.ResultsThe in situ simulation was appropriate for use within the participating General Practices. Qualitative thematic analysis of the interviews identified four themes: (1) apprehension and (un)willing participation, (2) reflection on the simulation design, (3) experiences of the scenario and (4) training.ConclusionsThis study suggests in situ simulation can be an acceptable approach for interdisciplinary team training within primary care, being well-received by practices and staff. This contributes to a fuller understanding of how in situ simulation can benefit both workforce and patients. Future research is needed to further refine the in situ simulation training session.


2020 ◽  
Vol 12 (558) ◽  
pp. eabc0441
Author(s):  
Junwei Li ◽  
Thomas Wang ◽  
Ameya R. Kirtane ◽  
Yunhua Shi ◽  
Alexis Jones ◽  
...  

Epithelial tissues line the organs of the body, providing an initial protective barrier as well as a surface for nutrient and drug absorption. Here, we identified enzymatic components present in the gastrointestinal epithelium that can serve as selective means for tissue-directed polymerization. We focused on the small intestine, given its role in drug and nutrient absorption and identified catalase as an essential enzyme with the potential to catalyze polymerization and growth of synthetic biomaterial layers. We demonstrated that the polymerization of dopamine by catalase yields strong tissue adhesion. We characterized the mechanism and specificity of the polymerization in segments of the gastrointestinal tracts of pigs and humans ex vivo. Moreover, we demonstrated proof of concept for application of these gastrointestinal synthetic epithelial linings for drug delivery, enzymatic immobilization for digestive supplementation, and nutritional modulation through transient barrier formation in pigs. This catalase-based approach to in situ biomaterial generation may have broad indications for gastrointestinal applications.


2017 ◽  
Vol 89 (7) ◽  
pp. 3810-3813 ◽  
Author(s):  
Shuo-Hui Cao ◽  
Zu-Rong Ni ◽  
Long Huang ◽  
Hui-Jun Sun ◽  
Biao Tang ◽  
...  

2019 ◽  
Vol 569 ◽  
pp. 118564 ◽  
Author(s):  
Fareeha Batool ◽  
Kevimy Agossa ◽  
Martin Lizambard ◽  
Catherine Petit ◽  
Isaac Maximiliano Bugueno ◽  
...  

Author(s):  
Patrick Kiele ◽  
David Braig ◽  
Jakob Weis ◽  
Yara Baslan ◽  
Cristian Pasluosta ◽  
...  

2016 ◽  
Vol 33 ◽  
pp. 23-28 ◽  
Author(s):  
Désirée Schneider ◽  
Kathrin Dennerlein ◽  
Thomas Göen ◽  
Karl Heinz Schaller ◽  
Hans Drexler ◽  
...  

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