A dual-signal fluorometric-colorimetric sensing platform and visual detection with a smartphone for the determination of β-galactosidase activity based on fluorescence silicon nanoparticles

Talanta ◽  
2022 ◽  
Vol 240 ◽  
pp. 123165
Jinying Liu ◽  
Ziwen Li ◽  
Jiabao Zhang ◽  
Guannan Wang ◽  
Xingguang Su
2020 ◽  
Vol 44 (22) ◽  
pp. 9223-9229 ◽  
Paweenar Duenchay ◽  
Kantima Kaewjua ◽  
Orawon Chailapakul ◽  
Weena Siangproh

A simple and fast alternative colorimetric sensing platform for selective determination of vitamin B1 in urine samples was proposed.

2020 ◽  
Vol 1 (8) ◽  
pp. 2789-2796
Liyun Lin ◽  
Huifang Ma ◽  
Chunliang Yang ◽  
Wuhai Chen ◽  
Shaodong Zeng ◽  

A Ce(iii)-driven self-assembled synthetic strategy was developed to fabricate 3D porous nanozymes. A paper-based, naked eye visible, disposable inhibition enzymatic nanoplatform was developed to detect organophosphate pesticides.

RSC Advances ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 4268-4276 ◽  
A. Anand Babu Christus ◽  
P. Panneerselvam ◽  
A. Ravikumar ◽  
M. Marieeswaran ◽  
S. Sivanesan

A novel colorimetric sensing platform using the peroxidase mimicking activity of ternary MoS2-loaded ZnO–g-C3N4 nanocomposites (ZnO–g-C3N4/MoS2) has been developed for the determination of Hg(ii) ions over co-existing metal ions.

2021 ◽  
Sahar Dadkhah ◽  
Ali Mehdinia ◽  
Ali Jabbari ◽  
Ahmad Manbohi

Abstract Development of an efficient, portable and simple nanosensor-based systems with reliable analytical performance for on-site monitoring of vitamin B12 (VB12) are still major problems and a challenging work for quality control of manufacturers. Herein, a new fluorescence, UV-Vis and smartphone triple mode nanosensors were designed for the simultaneous detection of VB12 with high sensitivity and accuracy. A novel nanosensor was synthesized through nicotinamide-functionalizing on carbon quantum dot (NA-CQDs) by an one-step microwave-assisted method with green approaches. The NA-CQDs as new nanosensor showed excellent fluorescence properties and wide linear ranges from 0.1–60 µM with the detection limits of 31.7 nM. Moreover, color changes of NA-CQDs induced by the VB12 could also be detected by UV-Vis spectrophotometer and inhouse-developed application installed on smartphone as a signal reader, simultanusly. The Red, Green and Blue (RGB) intensities of the colorimetric images of NA-CQDs/VB12 system which taken by smartphone's camera converted into quantitative values by the introduced application. A smartphone-integrated with NA-CQDs as colorimetric sensing platform displays good linear ranges (4.16 to 66.6 µM) for on-site determination of VB12 with detection limit of 1.40 µM. The method was successfully applied in the determination of VB12 in complex pharmaceutical supplement formulations without any sample pre-treatment and matrix interfering effects. The recovery results (96.52–105.10%) which are in agreement with the reference methods, demonstrating the capability of the smartphone-assisted colorimetric sensing platform in many on-site practical applications of quality controls.

2020 ◽  
Vol 16 ◽  
Biljana Nigović ◽  
Iva Šimunić ◽  
Ana Mornar

Background: Ondansetron and paracetamol are often co-administrated to prevent and treat nausea and vomiting caused by anaesthesia and to control of postoperative pain. In addition, ondansetron is used as the first-line antiemetic in paracetamol overdose. Therefore, selective and sensitive method for their simultaneous analysis is of a great importance. The electroanalytical methods are highly sensitive and offer many possibilities for new sensor platform design. However, at present, no electroanalytical method for simultaneous determination of these drugs has been proposed. Objective: The aim of this study was to develop a novel nanosensor for selective monitoring of ondansetron and paracetamol in pharmaceutical and biological samples without expensive and time-consuming pretreatments. Methods: The graphitized multi-walled carbon nanotubes embedded in a cation exchange polymer matrix was selected, among various surface functionalizations evaluated, to design novel sensor. Based on its excellent sensing performance, the first electroanalytical method was developed for rapid concurrent determination of investigated drugs. Results: The scanning electron microscopy study showed interlinked nanoporous network structure and highly enlarged active surface. The developed sensor facilitated electron transfer in the oxidation of both drugs and tremendously enhanced the adsorption capacity for ondasetron, thus exhibiting significant increase of drug responses and sensitivity. To obtain much sensitive response of investigated drugs the effect of pH values of supporting electrolyte, dispersed nanomaterial amount, the cation exchange polymer concentration, drop-casting volume of nanocomposite suspension, accumulation potential and deposition time on the peak current was evaluated. The developed electroanalytical method was validated and practical utility of the proposed nanosensor was tested. Conclusion: The developed sensor is promising sensing platform with a fast response time for analysis of ondansetron and paracetamol at very different concentration levels found in their fixed-dose combination and human serum sample after recommended daily doses showing its potential usage in pharmaceutical quality control and clinical research.

2021 ◽  
Vol 45 (6) ◽  
pp. 3215-3223
Selvarasu Maheshwaran ◽  
Ramachandran Balaji ◽  
Shen-Ming Chen ◽  
Ray Biswadeep ◽  
Vengudusamy Renganathan ◽  

A high-performance electrochemical sensing platform based on CuS nano-globules is efficiently developed.

2021 ◽  
Vol 262 ◽  
pp. 124289
Umar Nishan ◽  
Ujala Sabba ◽  
Abdur Rahim ◽  
Muhammad Asad ◽  
Mohibullah Shah ◽  

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 204
Yanyan Cheng ◽  
Ling Liang ◽  
Fanggui Ye ◽  
Shulin Zhao

Metal–organic framework (MOF) nanozymes, as emerging members of the nanozymes, have received more and more attention due to their composition and structural characteristics. In this work, we report that mixed-valence state Ce-MOF (MVCM) has intrinsic haloperoxidase-mimicking activity. MVCM was synthesized by partial oxidation method using Ce-MOF as a precursor. In the presence of H2O2 and Br−, MVCM can catalyze oxidative bromination of chromogenic substrate phenol red (PR) to produce the blue product bromophenol blue (Br4PR), showing good haloperoxidase-like activity. Because of the special chromogenic substrate, we constructed a ratiometric colorimetric-sensing platform by detecting the absorbance of the MVCM-(PR, Br−) system at wavelengths of 590 and 430, for quantifying H2O2, where the detection limit of the H2O2 is 3.25 μM. In addition, the haloperoxidase-mimicking mechanism of the MVCM is proposed. Moreover, through enzyme kinetics monitoring, the Km (H2O2 and NH4Br) of the MVCM is lower than that of cerium oxide nanomaterials, indicating that the MVCM has a stronger binding affinity for H2O2 and NH4Br than other materials. This work provides more application prospects for the development of nanozymes in the field of biosensors in the future.

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