An examination of quinone toxicity using the yeast Saccharomyces cerevisiae model system

Toxicology ◽  
2004 ◽  
Vol 201 (1-3) ◽  
pp. 185-196 ◽  
Author(s):  
Chester E Rodriguez ◽  
Masaru Shinyashiki ◽  
John Froines ◽  
Rong Chun Yu ◽  
Jon M Fukuto ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Model System ◽  
Yeast Saccharomyces Cerevisiae ◽  
Quinone Toxicity

scholarly journals Saccharomyces cerevisiae as a Tool to Investigate Plant Potassium and Sodium Transporters

2019 ◽  
Vol 20 (9) ◽  
pp. 2133 ◽  
Author(s):  
Antonella Locascio ◽  
Nuria Andrés-Colás ◽  
José Miguel Mulet ◽  
Lynne Yenush
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Interactions ◽  
Model System ◽  
Protein Protein Interactions ◽  
Alkali Cations ◽  
Yeast Saccharomyces Cerevisiae ◽  
Future Directions ◽  
Sodium Transporters ◽  
Adverse Environmental Conditions ◽  
Sodium And Potassium

Sodium and potassium are two alkali cations abundant in the biosphere. Potassium is essential for plants and its concentration must be maintained at approximately 150 mM in the plant cell cytoplasm including under circumstances where its concentration is much lower in soil. On the other hand, sodium must be extruded from the plant or accumulated either in the vacuole or in specific plant structures. Maintaining a high intracellular K+/Na+ ratio under adverse environmental conditions or in the presence of salt is essential to maintain cellular homeostasis and to avoid toxicity. The baker’s yeast, Saccharomyces cerevisiae, has been used to identify and characterize participants in potassium and sodium homeostasis in plants for many years. Its utility resides in the fact that the electric gradient across the membrane and the vacuoles is similar to plants. Most plant proteins can be expressed in yeast and are functional in this unicellular model system, which allows for productive structure-function studies for ion transporting proteins. Moreover, yeast can also be used as a high-throughput platform for the identification of genes that confer stress tolerance and for the study of protein–protein interactions. In this review, we summarize advances regarding potassium and sodium transport that have been discovered using the yeast model system, the state-of-the-art of the available techniques and the future directions and opportunities in this field.

scholarly journals Recombination of plasmids into the Saccharomyces cerevisiae chromosome is reduced by small amounts of sequence heterogeneity.

1983 ◽  
Vol 3 (7) ◽  
pp. 1204-1211 ◽  
Author(s):  
S Smolik-Utlaut ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Sequences ◽  
Recombinant Plasmid ◽  
Mitotic Recombination ◽  
Model System ◽  
Rrna Genes ◽  
Yeast Saccharomyces Cerevisiae ◽  
Sequence Heterogeneity ◽  
Recombinant Plasmids ◽  
Chromosomal Loci

As a model system for studying the properties of mitotic recombination in the yeast Saccharomyces cerevisiae, we have examined recombination between a recombinant plasmid (introduced into the S. cerevisiae cell by transformation) and homologous chromosomal loci. The recombinant plasmids used in these experiments contained S. cerevisiae rRNA genes. We found that the frequency of integrative recombination is sensitive to small amounts of sequence heterogeneity. In addition, the frequency and specificity of these recombination events are affected by the lengths of the interacting homologous DNA sequences.

Recombination of plasmids into the Saccharomyces cerevisiae chromosome is reduced by small amounts of sequence heterogeneity

1983 ◽  
Vol 3 (7) ◽  
pp. 1204-1211
Author(s):  
S Smolik-Utlaut ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Sequences ◽  
Recombinant Plasmid ◽  
Mitotic Recombination ◽  
Model System ◽  
Rrna Genes ◽  
Yeast Saccharomyces Cerevisiae ◽  
Sequence Heterogeneity ◽  
Recombinant Plasmids ◽  
Chromosomal Loci

As a model system for studying the properties of mitotic recombination in the yeast Saccharomyces cerevisiae, we have examined recombination between a recombinant plasmid (introduced into the S. cerevisiae cell by transformation) and homologous chromosomal loci. The recombinant plasmids used in these experiments contained S. cerevisiae rRNA genes. We found that the frequency of integrative recombination is sensitive to small amounts of sequence heterogeneity. In addition, the frequency and specificity of these recombination events are affected by the lengths of the interacting homologous DNA sequences.

scholarly journals Saccharomyces cerevisiae--a model organism for the studies on vacuolar transport.

2001 ◽  
Vol 48 (4) ◽  
pp. 1025-1042 ◽  
Author(s):  
R Kucharczyk ◽  
J Rytka
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Biology ◽  
Current Knowledge ◽  
Model Organism ◽  
Molecular Transport ◽  
Model System ◽  
Yeast Saccharomyces Cerevisiae ◽  
Vacuolar Transport ◽  
Vacuolar Trafficking

The role of the yeast vacuole, a functional analogue of the mammalian lysosome, in the turnover of proteins and organelles has been well documented. This review provides an overview of the current knowledge of vesicle mediated vacuolar transport in the yeast Saccharomyces cerevisiae cells. Due to the conservation of the molecular transport machinery S. cerevisiae has become an important model system of vacuolar trafficking because of the facile application of genetics, molecular biology and biochemistry.

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