Lipoxygenase-derived eicosanoids are involved in the inhibitory effect of Crotalus durissus terrificus venom or crotoxin on rat macrophage phagocytosis

The injection ofCrotalus durissus terrificusvenom into the foot pad of mice did not induce a significant inflammatory response as evaluated by oedema formation, increased vascular permeability and cell migration. The subcutaneous injection of the venom, or its addition to cell cultures, had an inhibitory effect on the spreading and phagocytosis of resident macrophages, without affecting the viability of the cells. This effect was not observed when the venom was added to cultures of thioglycollate elicited macrophages, but it was able to inhibit these macrophage functions when the cells were obtained from animals injected simultaneously with the venom and thioglycollate. These observations suggest that the venom interferes with the mechanisms of macrophage activation. Leukocyte migration induced by intraperitoneal injection of thioglycollate was also inhibited by previous venom injection. This down-regulatory activity of the venom on macrophage functions could account for the mild inflammatory response observed in the site of the snake bite inCrotalus durissus terrificusenvenomation in man.

Download Full-text

Effect of Isolated Proteins from Crotalus Durissus Terrificus Venom on Leishmania (Leishmania) Amazonensis-Infected Macrophages

Protein and Peptide Letters ◽

10.2174/0929866527666200129152954 ◽

2020 ◽

Vol 27 (8) ◽

pp. 718-724 ◽

Cited By ~ 1

Author(s):

Simone Katz ◽

Clara Lúcia Barbiéri ◽

Fernanda Paula Martins Soler ◽

Andreimar Martins Soares ◽

Maria Cristina Chavantes ◽

...

Keyword(s):

Cutaneous Leishmaniasis ◽

Inhibitory Effect ◽

Leishmania Amazonensis ◽

Significant Activity ◽

Crotalus Durissus Terrificus ◽

Mucocutaneous Leishmaniasis ◽

Potential Alternative ◽

Etiological Agents ◽

Crotalus Durissus

Background: Cutaneous and mucocutaneous leishmaniasis are parasitic diseases characterized by skin manifestations. In Brazil, Leishmania (Leishmania) amazonensis is one of the etiological agents of cutaneous leishmaniasis. The therapeutic arsenal routinely employed to treat infected patients is unsatisfactory, especially for pentavalent antimonials, as they are often highly toxic, poorly tolerated and of variable effectiveness. This study aimed to evaluate in vitro the leishmanicidal activity of toxins isolated from Crotalus durissus terrificus venom as a new approach for the treatment of leishmaniasis. Methods: The comparative effects of crotamine, crotoxin, gyrotoxin, convulxin and PLA2 on bone marrow-derived macrophages infected with L. (L.) amazonensis as well as the release of TGF-β from the treated macrophages were studied. Results and Discussion: Crotamine had the strongest inhibitory effect on parasite growth rate (IC50: 25.65±0.52 μg/mL), while convulxin showed the weakest inhibitory effect (IC50: 52.7±2.21 μg/mL). In addition, TGF-β was significantly reduced after the treatment with all toxins evaluated. Conclusion: The Crotalus durissus terrificus toxins used in this study displayed significant activity against L. (L.) amazonensis, indicating that all of them could be a potential alternative for the treatment of cutaneous leishmaniasis.

Download Full-text

L-aminoacid oxidase from Crotalus durissus terrificus snake venom and its antibacterial potential

Toxicon ◽

10.1016/j.toxicon.2019.06.071 ◽

2019 ◽

Vol 168 ◽

pp. S13

Author(s):

Leonardo Melo ◽

Lidiane Nunes Barbosa ◽

Rui Seabra Ferreira Junior ◽

Benedito Barraviera ◽

Luciana Curtolo De Barros ◽

...

Keyword(s):

Snake Venom ◽

Crotalus Durissus Terrificus ◽

Crotalus Durissus ◽

Antibacterial Potential

Download Full-text

Multiple effects of toxins isolated from Crotalus durissus terrificus on the hepatitis C virus life cycle

PLoS ONE ◽

10.1371/journal.pone.0187857 ◽

2017 ◽

Vol 12 (11) ◽

pp. e0187857 ◽

Cited By ~ 7

Author(s):

Jacqueline Farinha Shimizu ◽

Carina Machado Pereira ◽

Cintia Bittar ◽

Mariana Nogueira Batista ◽

Guilherme Rodrigues Fernandes Campos ◽

...

Keyword(s):

Life Cycle ◽

Hepatitis C Virus ◽

Hepatitis C ◽

Crotalus Durissus Terrificus ◽

Virus Life Cycle ◽

Crotalus Durissus

Download Full-text

Semen collection and evaluation in free-ranging Brazilian rattlesnakes (Crotalus durissus terrificus)

Zoo Biology ◽

10.1002/zoo.20126 ◽

2007 ◽

Vol 26 (2) ◽

pp. 155-160 ◽

Cited By ~ 19

Author(s):

Rogério Loesch Zacariotti ◽

Kathleen Fernandes Grego ◽

Wilson Fernandes ◽

Sávio Stefanini Sant'Anna ◽

Marcelo Alcindo de Barros Vaz Guimarães

Keyword(s):

Crotalus Durissus Terrificus ◽

Semen Collection ◽

Crotalus Durissus ◽

Free Ranging

Download Full-text

Inhibition of Neurotoxic Secretory Phospholipases A2Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated fromHarpalyce brasilianaBenth

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/987517 ◽

2012 ◽

Vol 2012 ◽

pp. 1-9 ◽

Cited By ~ 6

Author(s):

Rafael M. Ximenes ◽

Marcelo M. Rabello ◽

Renata M. Araújo ◽

Edilberto R. Silveira ◽

Fábio H. R. Fagundes ◽

...

Keyword(s):

Active Site ◽

Initial Step ◽

Significant Inhibition ◽

Lipid Mediators ◽

Naja Naja ◽

Crotalus Durissus Terrificus ◽

Docking Calculations ◽

Crotalus Durissus ◽

Bothrops Pirajai ◽

Phospholipases A

Secretory phospholipases A2(sPLA2) exert proinflammatory actions through lipid mediators. These enzymes have been found to be elevated in many inflammatory disorders such as rheumatoid arthritis, sepsis, and atherosclerosis. The aim of this study was to evaluate the effect of harpalycin 2 (Har2), an isoflavone isolated fromHarpalyce brasilianaBenth., in the enzymatic, edematogenic, and myotoxic activities of sPLA2fromBothrops pirajai, Crotalus durissus terrificus, Apis mellifera,andNaja najavenoms. Har2 inhibits all sPLA2tested. PrTX-III (B. pirajaivenom) was inhibited at about 58.7%, Cdt F15 (C. d. terrificusvenom) at 78.8%, Apis (from bee venom) at 87.7%, and Naja (N. najavenom) at 88.1%. Edema induced by exogenous sPLA2administration performed in mice paws showed significant inhibition by Har2 at the initial step. In addition, Har2 also inhibited the myotoxic activity of these sPLA2s. In order to understand how Har2 interacts with these enzymes, docking calculations were made, indicating that the residues His48 and Asp49 in the active site of these enzymes interacted powerfully with Har2 through hydrogen bonds. These data pointed to a possible anti-inflammatory activity of Har2 through sPLA2inhibition.

Download Full-text

Thermolability of 28S ribosomal ribonucleic acid from the liver of Crotalus durissus terrificus (Ophidia, Reptilia)

Biochemical Journal ◽

10.1042/bj1350073 ◽

1973 ◽

Vol 135 (1) ◽

pp. 73-79 ◽

Cited By ~ 8

Author(s):

J. F. Giorgini ◽

F. L. De Lucca

Keyword(s):

Molecular Weight ◽

Gradient Centrifugation ◽

Polyacrylamide Gel Electrophoresis ◽

Sucrose Density Gradient ◽

Dimethyl Sulphoxide ◽

Low Molecular Weight ◽

28S Rrna ◽

Sucrose Density Gradient Centrifugation ◽

Crotalus Durissus Terrificus ◽

Crotalus Durissus

Instability of 28S rRNA of Crotalus durissus terrificus liver was observed during hotphenol extraction: purified 28S rRNA is converted into an 18S RNA component by heat treatment. It was also found that ‘6S’ and ‘8S’ low-molecular-weight RNA species were released during the thermal conversion. This conversion and the release of the low-molecular-weight species were also induced by 8m-urea and 80% (v/v) dimethyl sulphoxide at 0°C. Evidence is presented that this phenomenon is an irreversible process and results from the rupture of hydrogen bonds. The 18S RNA product was shown to be homogeneous by polyacrylamide-gel electrophoresis and by sucrose-density-gradient centrifugation. The base composition of the 18S RNA products obtained by heat, urea or dimethyl sulphoxide treatments was similar. The C+G content of the 18S RNA product was different from that of the native 18S rRNA, but similar to that of 28S rRNA.

Download Full-text