The applicability of zebra fish animal models for acute toxicity testing of silver nanoparticle

2018 ◽  
Vol 295 ◽  
pp. S204 ◽  
Author(s):  
Y.-J. Wang ◽  
Y.-H. Lee
Keyword(s):  
Animal Models ◽  
Acute Toxicity ◽  
Silver Nanoparticle ◽  
Toxicity Testing ◽  
Zebra Fish ◽  
Acute Toxicity Testing

The Toxicity Profile of a Single Dose of Paroxetine: An Alternative Approach to Acute Toxicity Testing in the Rat

2001 ◽  
Vol 88 (2) ◽  
pp. 59-66 ◽  
Author(s):  
Pauline M. Ryan ◽  
John P. Kelly1Note ◽  
Philip L. Chambers ◽  
Brian E. Leonard
Keyword(s):  
Single Dose ◽  
Acute Toxicity ◽  
Toxicity Testing ◽  
Toxicity Profile ◽  
Alternative Approach ◽  
Acute Toxicity Testing

Acute Toxicity Testing with Juvenile White Sturgeon (Acipenser transmontanus)

1998 ◽  
Vol 33 (1) ◽  
pp. 95-110 ◽  
Author(s):  
W.R. Bennett ◽  
A.P. Farrell
Keyword(s):  
Acute Toxicity ◽  
Swimming Performance ◽  
Juvenile Fish ◽  
Toxicity Testing ◽  
White Sturgeon ◽  
Acipenser Transmontanus ◽  
Toxicity Tests ◽  
Fraser River ◽  
Acute Toxicity Tests ◽  
Acute Toxicity Testing

Abstract The primary goal of this study was to investigate the possibility of using early life stages of white sturgeon (Acipenser transmontanus) (eggs, larvae and fry) as a species relevant to the Fraser River, B.C., for the acute and sublethal toxico-logical testing of forest industry effluents. Here we report the first successful acute toxicity tests for 8-day-old larvae and 42-day-old fry exposed to several chemicals known to be released into the Fraser River (i.e., 6 monochlorovanillin [6 MVAN], 4,5 dichloroguaiacol [4,5 DCG], 4,5 dichlorocatechol [4,5 DCAT], pentachlorophenol [PCP], and didecyldimethylammonium chloride [DDAC]). In most cases, white sturgeon fry were at the lower end of the range for acute toxicity values for chlorinated phenolic compounds, when compared with other juvenile fish species, and they were extremely sensitive to DDAC. The larval stage was usually more sensitive than the fry stage. Acute toxicity tests with fertilized eggs were unsuccessful. A postexposure growth study was inconclusive because neither control nor toxicant-exposed larvae and fry withstood the additional handling used for measuring body mass. At 62-days-old, fry were more tolerant of handling. This allowed measurement of their swimming performance. Although we have concerns about the reliability of using larvae for acute toxicity testing at this time, 60-day-old white sturgeon fry would appear to be both a sensitive and relevant species for assessing environmental impacts relevant to the Fraser River.

The zebrafish embryo model in environmental risk assessment—applications beyond acute toxicity testing

2008 ◽  
Vol 15 (5) ◽  
pp. 394-404 ◽  
Author(s):  
Stefan Scholz ◽  
Stephan Fischer ◽  
Ulrike Gündel ◽  
Eberhard Küster ◽  
Till Luckenbach ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Acute Toxicity ◽  
Environmental Risk ◽  
Environmental Risk Assessment ◽  
Zebrafish Embryo ◽  
Toxicity Testing ◽  
Acute Toxicity Testing

The Biocompatibility and Security of Magnetic Nanoparticles Fe3O4-DNR Used In Hematologic Malignancies Therapy

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 3970-3970
Author(s):  
Bao-An Chen ◽  
Wei-wei Wu ◽  
Jian Cheng ◽  
Jun Wang ◽  
Feng Gao ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Acute Toxicity ◽  
Toxicity Testing ◽  
Hematologic Malignancies ◽  
Control Group ◽  
Significant Difference ◽  
Magnetic Nanoparticles Fe3o4 ◽  
Experimental Group ◽  
Fe3o4 Mnps ◽  
Acute Toxicity Testing

Abstract Abstract 3970 The objective of this paper is to study the biocompatibility and security of self-prepared magnetic nanoparticles Fe3O4 (Fe3O4-MNPs) loaded with daunorubicin (DNR), which has the potential application in hematologic malignancies therapy. Hemolysis test was carried out to estimate it s blood toxicity; Fe3O4-MNPs loaded with DNR were intra peritoneally injected into mouse to calculate the LD50; micronucleus (MN) assay was reckoned to identify its genotoxicity; acute toxicity testing was evaluated its influence to mouse hepatic and renal functions. The result of hemolysis rate (HR) of Fe3O4-MNPs loaded with DNR was 2.908%, far less than 5%. Therefore, we concluded that self-prepated Fe3O4-MNPs loaded with DNR nanoparticles had no hemolytic reaction, and they consistent with the requirement of hemolytic test of biomaterials. The LD50 of Fe3O4-MNPs loaded with DNR nanoparticles to the mice was 1009.71mg/kg (relative content of DNR was 10mg/kg) and the 95% confidence interval was 769.11∼1262.40mg/kg, it had no significant difference compared with LD50 of using DNR only, which data was 8.51mg/kg and the 95% confidence interval was 6.48∼10.37mg/kg, and it had wide safety value circumscription. In micronucleus assay, compared Fe3O4-MNPs loaded with DNR nanoparticles experimental groups with negative control group, we found that the result had no significant difference (P > 0. 05) in micronucleus formation rate, while compared experimental groups with positive control group, the result had significant difference (P < 0. 05). The result indicated that Fe3O4 magnetic nanoparticles had no cacogenesis and mutagenesis. Acute toxicity testing showed that mice body weigh of control group, Fe3O4-MNPs loaded with DNR nanoparticles experimental group and isodose DNR group had no significant difference in 24h, 48h, and 72h after intra peritoneally injection; they had normal activity, eating and evacuation; toxic reactions such as instability of gait, convulsion, paralysis and respiratory depression were not been found; the alanine transarninase (ALT), blood urea nitrogen (BUN), and creatinine clearance rate (CCr) of Fe3O4-MNPs loaded with DNR nanoparticles experimental group was 66.0±28.55u/L, 9.06±1.05mmol/L, and 18.03±1.84umol/L, respectively, which had no significant difference compared with control group and isodose DNR group. From the results of our experiment, we could consider that self-prepared Fe3O4-MNPs loaded with DNR nanoparticles is a kind of high biocompatibility and security materials and perhaps is suitable for further application in hematologic malignancies therapy. Disclosures: No relevant conflicts of interest to declare.

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