Diagnostic survey of bovine abortion with special reference to Neospora caninum infection: Importance, repeated abortion and concurrent infection in aborted fetuses in Southern Brazil

2006 ◽  
Vol 172 (1) ◽  
pp. 114-120 ◽  
Author(s):  
Luis G. Corbellini ◽  
Caroline A. Pescador ◽  
Fernanda Frantz ◽  
Elsio Wunder ◽  
David Steffen ◽  
...  
Keyword(s):  
Special Reference ◽  
Neospora Caninum ◽  
Concurrent Infection ◽  
Southern Brazil ◽  
Caninum Infection ◽  
Bovine Abortion

scholarly journals The prevalence of Neospora caninum antibodies in bulk milk of dairy herds in the CzechRepublic: a case report

2012 ◽  
Vol 50 (No. 12) ◽  
pp. 549-552 ◽  
Author(s):  
L. Hurkova ◽  
D. Halova ◽  
D. Modry
Keyword(s):  
Neospora Caninum ◽  
Protozoan Parasite ◽  
Elisa Test ◽  
Rapid Screening ◽  
Dairy Herds ◽  
Caninum Infection ◽  
Bulk Milk ◽  
Milk Elisa ◽  
Bovine Abortion ◽  
Bulk Milk Elisa

Neospora caninum is a protozoan parasite causing bovine abortion all over the world. The aim of this study was to investigate how common N. caninum infection is among dairy herds in the Czech Republic. Bulk milk samples were collected from 495 dairy herds and analysed for the presence of specific antibodies by a commercial N. caninum iscom ELISA. Five out of 495 dairy herds (1.01%) had percentage positive values ≥20 and were considered positive. In the positive herds, blood samples were collected from cows and pregnant heifers and analysed by the ELISA test. The within-herd seroprevalence ranged from 2.5 to 50%. The bulk milk ELISA could be a useful and inexpensive method for rapid screening of Neospora caninum infection in dairy herds in large areas.

scholarly journals Serological survey of Neospora caninum in small ruminants from Pernambuco State, Brazil

2011 ◽  
Vol 20 (3) ◽  
pp. 246-248 ◽  
Author(s):  
Antonio Amélia Santos Mucalane Tembue ◽  
Rafael Antonio de Nascimento Ramos ◽  
Thais Rabelo de Sousa ◽  
Alessandra Ribeiro Albuquerque ◽  
Alvimar José da Costa ◽  
...  
Keyword(s):  
Neonatal Mortality ◽  
Neospora Caninum ◽  
Small Ruminants ◽  
Antibody Test ◽  
Northeastern Brazil ◽  
Serological Survey ◽  
Caninum Infection ◽  
Bovine Abortion

Neospora caninum infection is an important cause of bovine abortion as well as neonatal mortality in goats and sheep. A serological survey for antibodies against N. caninum in goats and sheep was carried out in the municipality of Ibimirim, PE, Northeastern Brazil. The imunnofluoresce antibody test showed that 26.6% (85/319) of the goats and 64.2% (52/81) of the sheep were positive. Serologic reactivity was associated with age in goats (p < 0.01) and sheep (p > 0.05), with increasing rates in older animals. These results indicate exposure to N. caninum among small ruminants in the study area.

scholarly journals Dense-Granule Protein NcGRA7, a New Marker for the Serodiagnosis of Neospora caninum Infection in Aborting Cows

2007 ◽  
Vol 14 (12) ◽  
pp. 1640-1643 ◽  
Author(s):  
Penglong Huang ◽  
Min Liao ◽  
Houshuang Zhang ◽  
Eung-goo Lee ◽  
Yoshifumi Nishikawa ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Specific Antibody ◽  
Neospora Caninum ◽  
Dense Granule ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Caninum Infection ◽  
Recombinant Antigens ◽  
Granule Protein ◽  
Bovine Abortion

ABSTRACT To investigate whether the production of an antigen-specific antibody is associated with Neospora caninum-induced bovine abortion, 62 serum samples were tested with an enzyme-linked immunosorbent assay using the recombinant antigens NcSAG1, NcSRS2, and NcGRA7. Our study suggested that NcGRA7 would be a new marker for the serodiagnosis of N. caninum infection resulting in abortion.

Seroprevalence of Toxoplasma gondii and Neospora caninum infection of cats in Hungary

2008 ◽  
Vol 56 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Sándor Hornok ◽  
Renate Edelhofer ◽  
Anja Joachim ◽  
Róbert Farkas ◽  
Krisztián Berta ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Fluorescent Antibody Test ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Concurrent Infection ◽  
Indirect Fluorescent Antibody ◽  
Caninum Infection ◽  
Blood Samples ◽  
Feline Infectious Peritonitis

Blood samples were collected from 330 cats in Hungary in order to evaluate their seroconversion to Toxoplasma gondii and Neospora caninum using the indirect fluorescent antibody test (IFAT). The overall prevalence of toxoplasmosis was 47.6%, the prevalence being 22.4% among urban, 50% among suburban and 61.3% among rural animals. Significantly more cats had high IFAT titres (1:640 to 1:5120) in the countryside. Female cats were more frequently infected with T. gondii than males (53.3% vs. 39.3%), and seropositivity increased with the age of animals. The prevalence (0.6%) and titre (1:40) of antibodies to N. caninum was low. Sixty-two cats were also screened for seroconversion to feline infectious peritonitis (FIP) virus. Higher titres to T. gondii were more frequently detected among FIP-positive cats, but this difference was non-significant due to the small number of cats with concurrent infection.

scholarly journals Investigation of Neospora caninum Seroprevalence and Associa-tion with Reproductive Problems in Cows in Burdur Province of Turkey

2021 ◽  
Author(s):  
Onur Köse ◽  
Ramazan Adanır ◽  
Mesih Kocamüftüoğlu ◽  
Yunus Çetin
Keyword(s):  
Neospora Caninum ◽  
Age Groups ◽  
Competitive Elisa ◽  
Caninum Infection ◽  
Blood Samples ◽  
Elisa Kit ◽  
Significant Difference ◽  
Bovine Abortion ◽  
The Relationship ◽  
Sera Samples

Background: An apicomplexan protozoon Neospora caninum, causative agent of neosporosis, is recognized as one of the most common and important cause of sporadic and endemic bovine abortion and reduced reproductivity in dairy and beef cattle worldwide. The present study was conducted to investigate the relationship between N. caninum seroprevalence and infertility problems in 400 cows in Burdur city, Turkey. Methods: Blood samples were collected from vena jugularis into sterile serum tubes from 49 aborted, 58 infertil, 48 pregnant and 245 healthy cows for the findings of reproductive anamnesis during a period of March 2010 to March 2011. Sera samples were analyzed by competitive ELISA kit. Results: The seroprevalences were 7.7%, 6.4% and 4.2% in 2-4, ≤2 and ≥4 age groups respectively and no statistically significance observed between age groups. Seropositivity rates were 5.7%, 5.1%, 4.5%, 3.6% in Holstein, Montofon, cross-breeds and Simental breeds respectively. Seroprevalence differences was not statistically significant among cattle breeds. Antibodies to N. caninum were found in rates of 16.3%, 6.9%, 6.3%, 2.4% in aborted, infertile, pregnant and healthy cows respectively and there was a significant difference (P<0.01) between aborted and healthy animals. Seroprevalences were Yeşilova 10%, Gölhisar and Ağlasun 8%, Bucak, Çavdır and Kemer 4%, Karamanlı and Burdur Centrum 2%, according to districts. Conclusion: The seroprevalence of N. caninum was revealed in Burdur region. It was emphasized that N. caninum infection should not be ignored in reproductive problems, especially in abortion cases.

scholarly journals Can hierarchical modeling improve our understanding of bovine abortion due to Neospora caninum infection?

2017 ◽  
Vol 237 ◽  
pp. 77-82 ◽  
Author(s):  
Brayan Alexander Fonseca Martinez ◽  
Vanessa Bielefeldt Leotti ◽  
Mauro Riegert Borba ◽  
Gustavo de Sousa e Silva ◽  
Luís Gustavo Corbellini
Keyword(s):  
Hierarchical Modeling ◽  
Neospora Caninum ◽  
Caninum Infection ◽  
Bovine Abortion

scholarly journals Spatial distance between sites of sampling associated with genetic variation among Neospora caninum in aborted bovine foetuses from northern Italy

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Luca Villa ◽  
Pavlo Maksimov ◽  
Christine Luttermann ◽  
Mareen Tuschy ◽  
Alessia L. Gazzonis ◽  
...  
Keyword(s):  
Genetic Distance ◽  
Neospora Caninum ◽  
Geographic Distance ◽  
Rare Event ◽  
Northern Italy ◽  
Spatial Distance ◽  
Italian Regions ◽  
Bovine Abortion

Abstract Background Neospora caninum, a coccidian protozoan, represents an important cause of bovine abortion. Available N. caninum strains show considerable variation in vitro and in vivo, including different virulence in cattle. To which extent sexual recombination, which is possible in the intestines of domestic dogs and closely related carnivores as definitive hosts, contributes to this variation is not clear yet. Methods Aborted bovine foetuses were collected between 2015 and early 2019 from Italian Holstein Friesian dairy herds suffering from reproductive problems. A total of 198 samples were collected from 165 intensive farms located in Lombardy, northern Italy. N. caninum samples were subjected to multilocus-microsatellite genotyping using ten previously established microsatellite markers. In addition to our own data, those from a recent study providing data on five markers from other northern Italian regions were included and analysed. Results Of the 55 samples finally subjected to genotyping, 35 were typed at all or 9 out of 10 loci and their individual multilocus-microsatellite genotype (MLMG) determined. Linear regression revealed a statistically significant association between the spatial distance of the sampling sites with the genetic distance of N. caninum MLMGs (P < 0.001). Including data from this and a previous North Italian study into eBURST analysis revealed that several of N. caninum MLMGs from northern Italy separate into four groups; most of the samples from Lombardy clustered in one of these groups. Principle component analysis revealed similar clusters and confirmed MLMG groups identified by eBURST. Variations observed between MLMGs were not equally distributed over all loci, but predominantly observed in MS7, MS6A, or MS10. Conclusions Our findings confirm the concept of local N. caninum subpopulations. The geographic distance of sampling was associated with the genetic distance as determined by microsatellite typing. Results suggest that multi-parental recombination in N. caninum is a rare event, but does not exclude uniparental mating. More comprehensive studies on microsatellites in N. caninum and related species like Toxoplasma gondii should be undertaken, not only to improve genotyping capabilities, but also to understand possible functions of these regions in the genomes of these parasites.

Transplacental transmission of Neospora caninum to lambs in successive pregnancies of naturally infected sheep in Southern Brazil

2021 ◽  
Vol 23 ◽  
pp. 100537
Author(s):  
Kamila Alcalá Gonçalves Pereira ◽  
Renato Silva de Sousa ◽  
Mary Suzan Varaschin ◽  
Ana Paula Brenner Busch Becker ◽  
Alda Lúcia Gomes Monteiro ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Infected Sheep ◽  
Transplacental Transmission ◽  
Southern Brazil

scholarly journals Neospora caninum Infection in a Litter of Labrador Retriever Dogs in Denmark

1995 ◽  
Vol 36 (3) ◽  
pp. 387-391
Author(s):  
A. Flagstad ◽  
Η. Ε. Jensen ◽  
Ι. Bjerkàs ◽  
Κ. Rasmussen
Keyword(s):  
Neospora Caninum ◽  
Labrador Retriever ◽  
Caninum Infection

scholarly journals Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

2017 ◽  
Vol 26 (3) ◽  
pp. 292-298 ◽  
Author(s):  
Cesar Augusto Barbosa de Macedo ◽  
Madlaine Frigo Silveira Barbosa de Macedo ◽  
Ana Carolina Miura ◽  
Alessandra Taroda ◽  
Sergio Tosi Cardim ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Dairy Cows ◽  
High Frequency ◽  
Neospora Caninum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Southern Brazil ◽  
Chain Reaction ◽  
Santa Catarina ◽  
Tissue Samples ◽  
Polymerase Chain

Abstract The aim of the present study was to investigate the occurrence of N. caninum associated with abortions of dairy cattle from Santa Catarina state, southern Brazil by using enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and polymerase chain reaction (PCR). Blood from dairy cows that aborted along with intrathoracic fluid and tissue samples (brain, heart, liver, and lung) from their fetuses were collected and used for serology; PCR, histopathological, and immunohistochemistry (IHC) evaluations were also conducted. Twenty-one cows (51.2%) out of 41, and eight fetuses (26.7%) out of 30 were ELISA (HerdCheck, IDEXX) positive for N. caninum. Dams > 36 months of age had a higher risk of being serum positive than younger animals. PCR and IHC revealed that 38.8% (14/36) and 25.0% (9/36) of the fetuses were positive for N. caninum, respectively for each of the tests. Seropositive cows had a higher frequency of fetuses that were also positive by either intrathoracic fluid, PCR, or IHC. In summary, the present study observed a high frequency of N. caninum in abortions from dairy cows from southern Brazil, with a higher N. caninum prevalence found in cows that were older than 36 months. In addition, serology, PCR, and IHC should be used all together for better diagnosis of neosporosis in cattle.

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