Association of postpartum uterine diseases with lying time and metabolic profiles of multiparous Holstein dairy cows in the transition period

2020 ◽  
Vol 263 ◽  
pp. 105533
Author(s):  
L. Cattaneo ◽  
V. Lopreiato ◽  
E. Trevisi ◽  
A. Minuti
Keyword(s):  
Dairy Cows ◽  
Transition Period ◽  
Metabolic Profiles ◽  
Uterine Diseases ◽  
Holstein Dairy Cows

scholarly journals RELATIONSHIP BETWEEN BLOOD METABOLIC PROFILES AND MILK YIELD ASSOCIATED WITH PARITY AND STAGE OF LACTATION IN CROSSBRED DAIRY COWS IN BANGLADESH

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
L Naher ◽  
M. A. Samad ◽  
S. H. M. F. Siddiki ◽  
M. T. Islam
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Metabolic Profile ◽  
Transition Period ◽  
Dairy Herds ◽  
Metabolic Profiles ◽  
Milk Samples ◽  
Stage Of Lactation ◽  
Lactating Dairy Cows ◽  
Calcium Phosphorus

Background: Cattle cross-breeding program has been launched throughout Bangladesh but this program has still focused mainly on biological rather than economic evaluation. Currently, 30000 dairy farms with mainly cross-bred cows are in operation throughout the country and this intensive dairy farming system with high milk yielding dairy cows is supposed to be associated with high incidence of sub-clinical nutritional and metabolic diseases. Objectives: The objective of this study was to detect the relationship between major blood metabolic profiles and milk yield associated with parity and stage of lactation in cross-bred dairy cows. Materials and Methods: Blood samples of 220 apparently healthy lactating cross-bred dairy cows (HF x L, n = 190; SH x L, n = 20 and JS x L, n = 10) of 10 dairy herds were collected for metabolic profile test (MPT) in Bangladesh during the period from July to November 2016. The major metabolic profiles which are associated with milk fever (calcium, phosphorus & magnesium) and ketosis (glucose) were considered in this study. These biochemical parameters were estimated to detect the influence of risk factors including herds, breeds, parities and lactation age of cross-bred lactating dairy cows by using the commercial kits in spectrophotometer method. Results: The evaluation of the biochemical constituents of 220 cross-bred lactating dairy cows revealed that 30% (n = 66) had hypocalcaemia and 20.45% (n = 45) had hypoglycemia. Significantly lower levels of average calcium (7.93 ± 0.36 mg/dl; p < 0.024) and glucose (43.44 ± 3.63 mg/dl; p < 0.0001) values were recorded in HF x L cross-bred dairy herd of BAUDF, Mymensingh in comparison to other investigated nine dairy herds. The highest milk yield was recorded at 7th parity (16 +- 0.91 liter / day) and 1st week of lactation (17.33 +- 1.09 liter / day) were associated with low mean calcium (8.09 +- 0.85 mg / dl; 6.68 +- 0.13 mg/dl) and glucose (36.45 +- 7.67 mg/dl; 32.31 +- 3.90 mg / dl) levels in comparison to the respective values of different parity and lactation weeks. Conclusions: A relationship between blood metabolic profiles and milk yield associated with herd, breed, parity and lactation stages was recorded in lactating crossbred dairy cows. The evaluation of blood metabolites at different stages of lactation cycle especially at transition period is required to detect the nutritional and metabolic health for optimum milk production and to achieve maximum reproductive potential of high yielding dairy cattle. The readily available milk samples could be used as a biological fluid to monitor the health and nutritional status of dairy cows by using mid-infrared (MIR) spectroscopy method to prevent sub-clinical metabolic disorders. Therefore, well developed laboratories with necessary equipment, test kits and reagent should be provided to perform MPT in both the blood and milk samples for practical uses. Keywords: Compton metabolic profile test, Metabolic profile, Lactating cross-bred cows, Calcium, Phosphorus, Magnesium, Glucose, Parity, Lactation stages

scholarly journals Relationship between expression pattern of vitamin D receptor, 1 alpha-hydroxylase enzyme, and chemokine RANTES genes and selected serum parameters during transition period in Holstein dairy cows

2020 ◽  
Vol 7 (1) ◽  
pp. e000339
Author(s):  
Hala A R Saed ◽  
Hussam M M Ibrahim ◽  
Sabry A El-Khodery ◽  
Mohamed A Youssef
Keyword(s):  
Gene Expression ◽  
Vitamin D ◽  
Dairy Cows ◽  
Transition Period ◽  
Post Partum ◽  
Serum Levels ◽  
Vdr Gene ◽  
Genetic Expression ◽  
Enzyme Gene ◽  
Holstein Dairy Cows

ObjectivesThe objective of the present study was to evaluate the pattern of genetic expression of vitamin D receptor (VDR), 1 alpha-hydroxylase (1α-OHase) enzyme and chemokine regulated on activation normal T-cell expressed and secreted (RANTES) in peripheral blood of Holstein dairy cows during transition period.MethodsBlood samples were collected from 16 Holstein dairy cows at 3 weeks prior expected date of delivery (EDD), at the day of parturition, and 3 weeks post-partum for assessment of expression profile of studied genes using real-time PCR and measurement of glucose, ionized calcium (Ca), parathyroid hormone (PTH), inorganic phosphorous (P), sodium (Na), potassium (K), chloride (Cl), and magnesium (Mg) levels.ResultsCompared with 3 weeks prior EDD, VDR gene expression decreased significantly at the day of parturition then increased significantly at 3 weeks post-partum. The genetic expression of 1α-OHase enzyme as well as PTH, K, Na and Cl levels increased significantly at the day of parturition. The Ca level decreased significantly at the day of parturition then increased significantly at 3 weeks post-partum. The P level increased significantly at the day of parturition then decreased significantly at 3 weeks post-partum. Glucose level decreased significantly at the day of parturition and at 3 weeks post-partum. RANTES gene expression showed non-significant changes among the three different time points. The expression of VDR gene had a negative correlation with the expression of 1α-OHase enzyme gene, and serum levels of glucose, PTH, P and K, but had a positive correlation with the serum Ca level. The expression of 1α-OHase enzyme gene had a positive correlation with serum levels of PTH, P and K, but had a negative correlation with the serum Ca level.ConclusionsResults of the current study indicate the importance of monitoring the genetic expression of VDR and 1α-OHase enzyme as indicators of metabolic changes during transition period, suggesting that they are candidate genes to judge the health status of dairy cows during such period.

scholarly journals Dynamics of the microbiota found in the vaginas of dairy cows during the transition period: Associations with uterine diseases and reproductive outcome

2017 ◽  
Vol 100 (4) ◽  
pp. 3043-3058 ◽  
Author(s):  
M.L.S. Bicalho ◽  
T. Santin ◽  
M.X. Rodrigues ◽  
C.E. Marques ◽  
S.F. Lima ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Transition Period ◽  
Reproductive Outcome ◽  
Uterine Diseases

scholarly journals PSXIII-32 Effects of rumen-protected niacin on dry mater intake, rumination, and milk production in multiparous Holstein dairy cow during transition period

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 365-366
Author(s):  
Naren Gaowa ◽  
Erdan Wang ◽  
Shengli Li ◽  
Zhijun Cao ◽  
Yachun Wang ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Yield ◽  
Transition Period ◽  
Control Diet ◽  
Negative Energy ◽  
Control Group ◽  
Holstein Dairy Cow ◽  
Experimental Stage ◽  
Holstein Dairy Cows

Abstract The dairy cows decrease dry mater intake (DMI) before calving and are frequently in negative energy balance after calving. The objective of this study was to investigate the effect of rumen-protected niacin (NA) on DMI, rumination, and milk production in multiparous Holstein dairy cows during transition period. Eighteen multiparous Holstein dairy cows were allocated into 3 groups: (1) control diet (Con; n = 6), not supplemented with NA, (2) supplemented diet 1 (NA1; supplemented with 20 g NA/cow daily, n = 6), and (3) supplemented diet 2 (NA2; supplemented with 40 g NA/cow daily, n = 6). Experimental stage was from 49 d before calving until 21 d after calving. Daily TMR intake was monitored by Roughage Intake Control System, and daily rumination time was monitored by Hr-Tag rumination monitoring system and milk yield was recorded every day after calving. Blood samples were collected on d -49, -35, -21, -14, -7, 1, 3, 7, 14, 21, and 28 relative to parturition for quantifying concentrations of neuropeptide Y (NPY) and orexin A (OXA). The 20 g/day NA feeding increased DMI during transition period (P &lt; 0.05). The concentrations of NPY and OXA, which are the intake related hormones, were significant higher in NA1 than the other two groups in the first two weeks after calving (P &lt; 0.05). Moreover, the daily rumination time in NA1 was significantly higher than the control group from two weeks before calving to the first week after calving (P &lt; 0.01). The 20 g/day NA feeding significantly increased milk yield (P &lt; 0.05) compared to control group in early lactation. Overall, our study demonstrated that 20 g/day NA feeding could improve DMI and production in fresh cows, while those improvements were not detected with 40 g/day NA feeding.

scholarly journals Metabolites able to predict uterine diseases in crossbred dairy cows during the transition period

2018 ◽  
Vol 39 (3) ◽  
pp. 1037 ◽  
Author(s):  
Erick Daibert ◽  
Paula Batista de Alvarenga ◽  
Amanda Lima Rezende ◽  
Nádia Simarro Fagundes ◽  
Barbara Cristina Krüger ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Transition Period ◽  
Body Condition Score ◽  
Negative Energy ◽  
Transitional Period ◽  
Holstein Cows ◽  
Crossbred Cows ◽  
Significant Difference ◽  
Beta Hydroxybutyrate ◽  
Uterine Diseases

This study aimed to identify possible metabolites able to predict puerperal uterine diseases in crossbred dairy cows (Holstein x Gir) in the transition period. We hypothesized that, as what has been observed for Holstein cows, a negative energy balance (NEB) may increase the incidence of uterine diseases. Thirty-four lactating crossbred cows (½HG, ¼HG, ¾HG, and 5/8HG) were evaluated during prepartum (-60d and -30d), calving, and postpartum (+14d, +21d, and +35d) periods, being divided into two groups (with and without uterine diseases). NEB evaluations were based on the concentrations of non-esterified fatty acids (NEFA) and beta-hydroxybutyrate (BHBA) at calving and 14 days postpartum. Nearly 1/3 of the crossbred cows presented an NEB, characterized by a significant increase in NEFA with no BHBA increase; however, it was less intense than that observed in Holstein cows during the transition period. There was a significant difference between cows with and without uterine diseases in NEFA and calcium concentrations at partum, as well as body condition score (BCS) during prepartum. In conclusion, the occurrence of uterine diseases during the transitional period is related to the BCS in the prepartum period, besides serum NEFA and calcium concentrations at partum.

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