Coordination of lipid droplet-associated proteins during the transition period of Holstein dairy cows

ObjectivesThe objective of the present study was to evaluate the pattern of genetic expression of vitamin D receptor (VDR), 1 alpha-hydroxylase (1α-OHase) enzyme and chemokine regulated on activation normal T-cell expressed and secreted (RANTES) in peripheral blood of Holstein dairy cows during transition period.MethodsBlood samples were collected from 16 Holstein dairy cows at 3 weeks prior expected date of delivery (EDD), at the day of parturition, and 3 weeks post-partum for assessment of expression profile of studied genes using real-time PCR and measurement of glucose, ionized calcium (Ca), parathyroid hormone (PTH), inorganic phosphorous (P), sodium (Na), potassium (K), chloride (Cl), and magnesium (Mg) levels.ResultsCompared with 3 weeks prior EDD, VDR gene expression decreased significantly at the day of parturition then increased significantly at 3 weeks post-partum. The genetic expression of 1α-OHase enzyme as well as PTH, K, Na and Cl levels increased significantly at the day of parturition. The Ca level decreased significantly at the day of parturition then increased significantly at 3 weeks post-partum. The P level increased significantly at the day of parturition then decreased significantly at 3 weeks post-partum. Glucose level decreased significantly at the day of parturition and at 3 weeks post-partum. RANTES gene expression showed non-significant changes among the three different time points. The expression of VDR gene had a negative correlation with the expression of 1α-OHase enzyme gene, and serum levels of glucose, PTH, P and K, but had a positive correlation with the serum Ca level. The expression of 1α-OHase enzyme gene had a positive correlation with serum levels of PTH, P and K, but had a negative correlation with the serum Ca level.ConclusionsResults of the current study indicate the importance of monitoring the genetic expression of VDR and 1α-OHase enzyme as indicators of metabolic changes during transition period, suggesting that they are candidate genes to judge the health status of dairy cows during such period.

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Gain and loss of subcutaneous and abdominal adipose tissue depot mass of German Holstein dairy cows with different body conditions during the transition period

Journal of Dairy Science ◽

10.3168/jds.2019-17623 ◽

2020 ◽

Vol 103 (12) ◽

pp. 12015-12032

Author(s):

G. Szura ◽

S. Schäfers ◽

D. von Soosten ◽

U. Meyer ◽

J. Klüß ◽

...

Keyword(s):

Adipose Tissue ◽

Dairy Cows ◽

Transition Period ◽

Abdominal Adipose Tissue ◽

Gain And Loss ◽

Adipose Tissue Depot ◽

Holstein Dairy Cows

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Association of postpartum uterine diseases with lying time and metabolic profiles of multiparous Holstein dairy cows in the transition period

The Veterinary Journal ◽

10.1016/j.tvjl.2020.105533 ◽

2020 ◽

Vol 263 ◽

pp. 105533

Author(s):

L. Cattaneo ◽

V. Lopreiato ◽

E. Trevisi ◽

A. Minuti

Keyword(s):

Dairy Cows ◽

Transition Period ◽

Metabolic Profiles ◽

Uterine Diseases ◽

Holstein Dairy Cows

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Serotonin receptor expression is dynamic in the liver during the transition period in Holstein dairy cows

Domestic Animal Endocrinology ◽

10.1016/j.domaniend.2014.11.005 ◽

2015 ◽

Vol 51 ◽

pp. 65-73 ◽

Cited By ~ 7

Author(s):

J. Laporta ◽

L.L. Hernandez

Keyword(s):

Dairy Cows ◽

Serotonin Receptor ◽

Transition Period ◽

Receptor Expression ◽

Holstein Dairy Cows

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PSXIII-32 Effects of rumen-protected niacin on dry mater intake, rumination, and milk production in multiparous Holstein dairy cow during transition period

Journal of Animal Science ◽

10.1093/jas/skz258.729 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 365-366

Author(s):

Naren Gaowa ◽

Erdan Wang ◽

Shengli Li ◽

Zhijun Cao ◽

Yachun Wang ◽

...

Keyword(s):

Dairy Cows ◽

Milk Production ◽

Milk Yield ◽

Transition Period ◽

Control Diet ◽

Negative Energy ◽

Control Group ◽

Holstein Dairy Cow ◽

Experimental Stage ◽

Holstein Dairy Cows

Abstract The dairy cows decrease dry mater intake (DMI) before calving and are frequently in negative energy balance after calving. The objective of this study was to investigate the effect of rumen-protected niacin (NA) on DMI, rumination, and milk production in multiparous Holstein dairy cows during transition period. Eighteen multiparous Holstein dairy cows were allocated into 3 groups: (1) control diet (Con; n = 6), not supplemented with NA, (2) supplemented diet 1 (NA1; supplemented with 20 g NA/cow daily, n = 6), and (3) supplemented diet 2 (NA2; supplemented with 40 g NA/cow daily, n = 6). Experimental stage was from 49 d before calving until 21 d after calving. Daily TMR intake was monitored by Roughage Intake Control System, and daily rumination time was monitored by Hr-Tag rumination monitoring system and milk yield was recorded every day after calving. Blood samples were collected on d -49, -35, -21, -14, -7, 1, 3, 7, 14, 21, and 28 relative to parturition for quantifying concentrations of neuropeptide Y (NPY) and orexin A (OXA). The 20 g/day NA feeding increased DMI during transition period (P < 0.05). The concentrations of NPY and OXA, which are the intake related hormones, were significant higher in NA1 than the other two groups in the first two weeks after calving (P < 0.05). Moreover, the daily rumination time in NA1 was significantly higher than the control group from two weeks before calving to the first week after calving (P < 0.01). The 20 g/day NA feeding significantly increased milk yield (P < 0.05) compared to control group in early lactation. Overall, our study demonstrated that 20 g/day NA feeding could improve DMI and production in fresh cows, while those improvements were not detected with 40 g/day NA feeding.

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RELATIONSHIP BETWEEN RETAINED PLACENTA AND BODY CONDITION CHANGES DURING THE TRANSITION PERIOD IN HOLSTEIN DAIRY COWS IN NORTH-EASTERN ALGERIA

SPERMOVA ◽

10.18548/aspe/0009.03 ◽

2021 ◽

Vol 11 (1) ◽

pp. 17-23

Author(s):

Ahmed Hadef ◽

◽

Kamel Miroud ◽

Hocine Sebihi

Keyword(s):

Dairy Cows ◽

Body Fat ◽

Body Condition ◽

Transition Period ◽

The Body ◽

Retained Placenta ◽

North Eastern ◽

Eastern Algeria ◽

The Impact ◽

Holstein Dairy Cows

Retained placenta (RP) is a common complication of bovine parturition. It is a multifactorial reproductive disorder that predisposes to infection and hence, to infertility. The impact of body condition changes during the transition period on the risk of RP appearance was evaluated in 12 Holstein dairy cows calving from October to January in north-eastern Algeria. The animals were body condition scored (BCS) 3 weeks antepartum and two weeks postpartum to assess the peripartum body fat loss (BCS loss). The statistical analysis involving variables related to BCS and parity was performed using the principal component analysis (PCA). An RP critical occurrence of 41.66% exceeding significantly the threshold prevalence of 10% (p<0.05) was recorded. This could be related to the BCS loss that exceeded one unit over the transition period (r=0.84, p<0.01), expressing the high body fat mobilisation in response to stressor factors such as negative energy balance, feed availability and climatic condition. Scoring the body condition, particularly around the peripartum, may serve as a tool to monitor the risk factors related to the energetic status of dairy cows.

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