scholarly journals Stimulation of biosurfactant production by Lactobacillus plantarum using ultrasound

2019 ◽  
Vol 59 ◽  
pp. 104724 ◽  
Author(s):  
Asma Behzadnia ◽  
Marzieh Moosavi-Nasab ◽  
Brijesh K. Tiwari
Keyword(s):  
Lactobacillus Plantarum ◽  
Biosurfactant Production ◽  
Stimulation Of

Improving salinity resilience in Swertia chirayita clonal line with Lactobacillus plantarum

2016 ◽  
Vol 96 (1) ◽  
pp. 117-127 ◽  
Author(s):  
Susanna Phoboo ◽  
Dipayan Sarkar ◽  
Prasanta C. Bhowmik ◽  
Pramod Kumar Jha ◽  
Kalidas Shetty
Keyword(s):  
Dehydrogenase Activity ◽  
Lactobacillus Plantarum ◽  
Salinity Stress ◽  
Pentose Phosphate Pathway ◽  
Krebs Cycle ◽  
Defense Responses ◽  
Pentose Phosphate ◽  
Clonal Line ◽  
Swertia Chirayita ◽  
Stimulation Of

Plants defense responses to abiotic stresses, including salinity stress, involve stimulation of defense related pathways such as biosynthesis of secondary metabolites and induction of endogenous antioxidant enzyme responses. In the present study, a single seed origin clonal line of Swertia chirayita inoculated with Lactobacillus plantarum (LP) was grown under different salinity levels. Control had no LP inoculation. S. chirayita inoculated with LP showed higher accumulation of proline, low proline dehydrogenase activity, up-regulation of pentose phosphate pathway, down-regulation of succinate dehydrogenase activity (Krebs cycle) and low total phenolic content with increased salt concentrations. In comparison, S. chirayita without LP adopted a different biochemical mechanism to counter salt stress (NaCl) by up-regulating both pentose phosphate pathway and Krebs cycle along with stimulation of phenolic biosynthesis. Guaiacol peroxidase (GPX) activity increased with and without LP treatment in response to increasing concentrations of salt. These results indicate that S. chirayita inoculated with LP exhibits a greater salinity stress tolerance than S. chirayita without LP by adopting a more energy efficient defense responses and potentially efficiently partitioning carbon flux between primary and secondary metabolism to counter salt induced oxidative stress.

Heat-killed Lactobacillus plantarum L-137 suppresses naturally fed antigen–specific IgE production by stimulation of IL-12 production in mice

1998 ◽  
Vol 102 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Shinji Murosaki ◽  
Yoshihiro Yamamoto ◽  
Kazue Ito ◽  
Takeaki Inokuchi ◽  
Hiroaki Kusaka ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Specific Ige ◽  
Stimulation Of

Effect of Major Spices in Lebanon Bologna on Acid Production by Starter Culture Organisms

1978 ◽  
Vol 41 (6) ◽  
pp. 429-431 ◽  
Author(s):  
J. C. KISSINGER ◽  
L. L. ZAIKA
Keyword(s):  
Liquid Medium ◽  
Lactobacillus Plantarum ◽  
Acid Production ◽  
Starter Culture ◽  
Antimicrobial Properties ◽  
Black Pepper ◽  
Plate Counts ◽  
Stimulation Of

The effect of a Lebanon bologna spice mixture and its major component spices, black pepper, allspice, and nutmeg, on acid production by a mixed starter culture containing Lactobacillus plantarum and Pediococcus cerevisiae was studied in a liquid medium. These spices stimulated acid production by the starter culture organisms although some Lebanon bologna component spices are known to have antimicrobial properties. The spice mixture stimulated L. plantarum more than P. cerevisiae when each organism was cultured singly. Stimulation of acid production could not be attributed solely to differences in bacterial numbers as defined by plate counts.

scholarly journals DETERMINATION OF THE OPTIMUM CONDITIONS FOR BIOSURFACTANT PRODUCTION BY LOCAL ISOLATE OF LACTOBACILLUS PLANTARUM AND EVALUATE ITS ANTIMICROBIAL ACTIVITY

2021 ◽  
Vol 52 (1) ◽  
pp. 170-188
Author(s):  
Jameel & Haider
Keyword(s):  
Lactobacillus Plantarum ◽  
Mineral Salt Medium ◽  
Biosurfactant Production ◽  
Production Medium ◽  
Optimum Conditions ◽  
Meat Extract ◽  
Primary Screening ◽  
Secondary Screening ◽  
Different Sources

Eighty five local isolates of Lactobacillus sp. which were isolated from different sources and identified by biochemical test then subjected to the primary and secondary screening processes to select the active Lactobacillus sp. isolate for biosurfactant production. Among the isolates screened, twenty six isolates with maximum for tests in primary screening were selected for secondary screening. It has been found that Lactobacillus sp. ADK2  had the highest productivity of the biosurfactant. The selected isolate with highest level of biosurfactant activity was identified as Lactobacillus plantarum ADK2 according to PCR technique. The optimum conditions of biosurfactant production by isolate Lactobacillus plantarum ADK2 using submerged fermentation were obtained in the synthetic mineral salt medium (MSM) and natural BCDFTM medium the best production medium separately, 1.5% (lactose and egg) as the best carbon source, 2% meat extract and 3.5% Pease as nitrogen source, temperature 30 °C for two media and pH 5 with pH 3 in MSM  and BCDFTM respectively, after 96 hr  and 72 hr in MSM and BCDFTM respectively of incubation period.

scholarly journals СТИМУЛЯЦИЯ УЛЬТРАЗВУКОМ НАКОПЛЕНИЯ БИОМАССЫ МОЛОЧНОКИСЛЫХ И ПРОПИОНОВОКИСЛЫХ БАКТЕРИЙ ПРИ ГЛУБИННОМ КУЛЬТИВИРОВАНИИ

2016 ◽  
Vol 6 (2) ◽  
pp. 287-296 ◽  
Author(s):  
D. A. Durnikin ◽  
M. M. Silantyeva ◽  
O. V. Ereshchenko
Keyword(s):  
Energy Density ◽  
Lactobacillus Plantarum ◽  
Lactococcus Lactis ◽  
New Technologies ◽  
Bacterial Biomass ◽  
Biomass Accumulation ◽  
Culture Broth ◽  
Ultrasound Stimulation ◽  
The Impact ◽  
Stimulation Of

<p>Lactic and propionic bacteria are actively used as feed and food biopreservatives. The industrial production of these bacteria is carried out using known standard biotechnological approaches and equipment. However, the modern requirements to the volumes of their production require the development of new technologies providing the more intensive growth of bacterial biomass. One of the possible ways to do it is the use of nonspecific stimulators of chemical or physical origin. The stimulating effect of ultrasound on live systems attracts attention of many researchers. Depending on the sonication parameters and conditions, the impact of ultrasound on cell cultures can either stimulate or suppress their life processes. The possibility of the ultrasound stimulation of the biomass accumulation process has been studied for submerged bacterial cultures of <em>Lactococcus lactis </em>VPKM B-2092<em>, Lactobacillus plantarum</em> VPKM B-4173<em>, </em>and <em>Propionibacterium</em><em> </em><em>acidipropionici </em>VPKM B-2092<em>. </em>The inoculum with cell contentration of 1 · 10<sup>8</sup> mL<sup>-1</sup> was sonicated at 880 kHz and energy density varied within 0.1-0.7 W/cm<sup>3</sup> using a specially designed cuvette, through which the cell suspension was introduced into a fermenter at a rate of 10 mL/s that provided the total sonication time equal to 100-120 seconds. As a signal source, a standard therapeutical ultrasound apparatus UZT-1.01F equipped with a sweep generator was used.</p><p>For all three cultures, the ultrasound stimulation resulted in a significant increase in the optical density of culture broth comparing to the control and the corresponding increase of the cell concentration. The optimum sonication energy density for the <em>Lactococcus lactis </em>VPKM B-2092<em>, Lactobacillus plantarum</em> VPKM B-4173<em>, </em>and <em>Propionibacterium</em><em> </em><em>acidipropionici </em>VPKM B-2092 was equal to 0.5, 0.3-0.5 и 0.3<em> </em>W/cm<sup>3</sup>, respectively. Comparing to the control, the cell count of these strains in the culture broth increased in 28.6, 9, and 16.7 times, respectively.</p><p>Thus, the ultrasound stimulation of inoculum provides a significant increase in the biomass of cells producing lactic and propionic acid that, in turn, increases the economic efficiency of their industrial use. Since the mechanisms of such stimulating action of ultrasound are well-studied, and the exploitation of ultrasound generators is simple and cheap, the further development of the ultrasound stimulation approach seems to be very promising for the industrial microbiology.</p><p> </p>

Effects of 5-5'-Diphenyl-2-thiohydantoin (DPTH) and Thyroxine on the Ultrastructure and Chemical Composition of Rat Liver

1971 ◽  
Vol 29 ◽  
pp. 570-571
Author(s):  
E. A. Elfont ◽  
R. B. Tobin ◽  
D. G. Colton ◽  
M. A. Mehlman
Keyword(s):  
Chemical Composition ◽  
Rat Liver ◽  
Future Study ◽  
Mode Of Action ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Effective Inhibitor ◽  
Biochemical Effects ◽  
Glycerophosphate Dehydrogenase ◽  
Stimulation Of

Summary5,-5'-diphenyl-2-thiohydantoin (DPTH) is an effective inhibitor of thyroxine (T4) stimulation of α-glycerophosphate dehydrogenase in rat liver mitochondria. Because this finding indicated a possible tool for future study of the mode of action of thyroxine, the ultrastructural and biochemical effects of DPTH and/or thyroxine on rat liver mere investigated.Rats were fed either standard or DPTH (0.06%) diet for 30 days before T4 (250 ug/kg/day) was injected. Injection of T4 occurred daily for 10 days prior to sacrifice. After removal of the liver and kidneys, part of the tissue was frozen at -50°C for later biocheailcal analyses, while the rest was prefixed in buffered 3.5X glutaraldehyde (390 mOs) and post-fixed in buffered 1Z OsO4 (376 mOs). Tissues were embedded in Araldlte 502 and the sections examined in a Zeiss EM 9S.Hepatocytes from hyperthyroid rats (Fig. 2) demonstrated enlarged and more numerous mitochondria than those of controls (Fig. 1). Glycogen was almost totally absent from the cytoplasm of the T4-treated rats.

Gap junctions in the prothoracic glands of the tobacco hornworm, Manduca sexta

1992 ◽  
Vol 50 (1) ◽  
pp. 706-707
Author(s):  
Ji-da Dai ◽  
M. Joseph Costello ◽  
Lawrence I. Gilbert
Keyword(s):  
Gap Junctions ◽  
Small Molecules ◽  
Manduca Sexta ◽  
Freeze Fracture ◽  
Cell Communication ◽  
Cellular Level ◽  
Thin Sections ◽  
Prothoracic Glands ◽  
Polyhydroxylated Steroids ◽  
Stimulation Of

Insect molting and metamorphosis are elicited by a class of polyhydroxylated steroids, ecdysteroids, that originate in the prothoracic glands (PGs). Prothoracicotropic hormone stimulation of steroidogenesis by the PGs at the cellular level involves both calcium and cAMP. Cell-to-cell communication mediated by gap junctions may play a key role in regulating signal transduction by controlling the transmission of small molecules and ions between adjacent cells. This is the first report of gap junctions in the PGs, the evidence obtained by means of SEM, thin sections and freeze-fracture replicas.

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