Production of biologically active peptides by hydrolysis of whey protein isolates using hydrodynamic cavitation

In recent years, there has been a steady increase in the number of various allergic diseases in children and adults. Clinical manifestations of allergic reactions can be observed almost from infancy, which is often associated with nutrition specificity. It is proved that one of the strongest milk allergens are the main whey fractions β-lactoglobulin and α-lactalbumin. One way to reduce allergies is to remove or completely replace whey proteins. Another way to reduce allergenicity is to use whey protein hydrolysates. In most cases, hydrolysates are obtained using cheap active proteolytic preparations. They usually have wide specificity, and the hydrolysates formed by them include short peptides with low allergenicity. Numerous biologically active peptides which have a positive effect on the organism are either lost or the possibility of their formation is reduced in such cases. A valuable source of such biologically active peptides is whey protein-precursors. Therefore, the purpose of the research is to develop a technology of low-allergenic milk with the preservation of natural biologically active peptides from whey proteins. The concentrate of natural casein was obtained in the result of the «milk-pectin-water» system dividing for creating a low-allergenic product. The fractional composition of the casein complex and milk whey proteins was analyzed by polyacrylamide gel electrophoresis. Whey protein concentrate was used as the substrate to obtain the hydrolysate. Proteolysis was performed with pancreatin at 37 °C and pH 7.9. The composition of the obtained hydrolysate was analyzed by gel filtration on Sephadex G-25. The peptides and polypeptides composition of hydrolysate was characterizated with the help of express electrophoresis. The technology of the proposed dairy product includes addition to a liquid concentrate of natural casein the whey protein hydrolysate obtained in physiological conditions. The results of electrophoretic and chromatographic researches indicate about the absence of main proteins allergens from milk whey in the product. This allows recommending it for the nutrition of people allergic to whey proteins.

Download Full-text

Continuous Enzymatic Hydrolysis of β-Casein and Isoelectric Collection of Some of the Biologically Active Peptides in an Electric Field

Biotechnology Progress ◽

10.1021/bp970019e ◽

1997 ◽

Vol 13 (3) ◽

pp. 258-264 ◽

Cited By ~ 27

Author(s):

P.G. Righetti ◽

F. Nembri ◽

A. Bossi ◽

M. Mortarino

Keyword(s):

Enzymatic Hydrolysis ◽

Electric Field ◽

Biologically Active ◽

Active Peptides ◽

Biologically Active Peptides ◽

Hydrolysis Of

Download Full-text

The Hydrolysis of Biologically Active Peptides by Bovine Lung Tissue Factor (Thromboplastin)

Experimental Biology and Medicine ◽

10.3181/00379727-152-39444 ◽

1976 ◽

Vol 152 (4) ◽

pp. 576-584 ◽

Cited By ~ 1

Author(s):

W. H. Simmons ◽

D. E. Burkholder ◽

A. S. Brecher

Keyword(s):

Tissue Factor ◽

Lung Tissue ◽

Biologically Active ◽

Active Peptides ◽

Biologically Active Peptides ◽

Hydrolysis Of

Download Full-text

The Influence of Enzymatic Hydrolysis of Whey Proteins on the Properties of Gelatin-Whey Composite Hydrogels

Materials ◽

10.3390/ma14133507 ◽

2021 ◽

Vol 14 (13) ◽

pp. 3507

Author(s):

Violeta Popescu ◽

Andreia Molea ◽

Marioara Moldovan ◽

Pompilia Mioara Lopes ◽

Amalia Mazilu Moldovan ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Whey Protein ◽

Whey Proteins ◽

Protein Hydrolysates ◽

Bacterial Invasion ◽

Swelling Kinetics ◽

Protein Isolates ◽

Vis Spectroscopy ◽

Whey Protein Isolates ◽

Hydrolysis Of

Amino-acids, peptides, and protein hydrolysates, together with their coordinating compounds, have various applications as fertilizers, nutritional supplements, additives, fillers, or active principles to produce hydrogels with therapeutic properties. Hydrogel-based patches can be adapted for drug, protein, or peptide delivery, and tissue healing and regeneration. These materials have the advantage of copying the contour of the wound surface, ensuring oxygenation, hydration, and at the same time protecting the surface from bacterial invasion. The aim of this paper is to describe the production of a new type of hydrogel based on whey protein isolates (WPI), whey protein hydrolysates (WPH), and gelatin. The hydrogels were obtained by utilizing a microwave-assisted method using gelatin, glycerol, WPI or WPH, copper sulfate, and water. WPH was obtained by enzymatic hydrolysis of whey protein isolates in the presence of bromelain. The hydrogel films obtained have been characterized by FT-IR and UV-VIS spectroscopy. The swelling degree and swelling kinetics have also been determined.

Download Full-text