Cynomolgus macaques immunized with two HIV-1 Tat stabilized proteins raise strong and long-lasting immune responses with a pattern of Th1/Th2 response differing from that in mice

Vaccine ◽  
2009 ◽  
Vol 27 (39) ◽  
pp. 5349-5356 ◽  
Author(s):  
Sabrina Turbant ◽  
Frédéric Martinon ◽  
Gervaise Moine ◽  
Roger Le Grand ◽  
Michel Léonetti
Keyword(s):  
Immune Responses ◽  
Th2 Response ◽  
Cynomolgus Macaques ◽  
Hiv 1

scholarly journals Induction of Broad Cross-Subtype-Specific HIV-1 Immune Responses by a Novel Multivalent HIV-1 Peptide Vaccine in Cynomolgus Macaques

2008 ◽  
Vol 180 (4) ◽  
pp. 2174-2186 ◽  
Author(s):  
Ali Azizi ◽  
David E. Anderson ◽  
José V. Torres ◽  
Andrei Ogrel ◽  
Masoud Ghorbani ◽  
...  
Keyword(s):  
Immune Responses ◽  
Peptide Vaccine ◽  
Cynomolgus Macaques ◽  
Hiv 1

scholarly journals Persistent Immunogenicity of Integrase Defective Lentiviral Vectors delivering membrane tethered Native-Like HIV-1 Envelope Trimers

2021 ◽  
Author(s):  
Alessandra Gallinaro ◽  
Maria Franca Pirillo ◽  
Yoann Aldon ◽  
Serena Cecchetti ◽  
Zuleika Michelini ◽  
...  
Keyword(s):  
Immune Responses ◽  
Lentiviral Vectors ◽  
Igg Antibodies ◽  
Vaccine Platform ◽  
Neutralization Activity ◽  
Cynomolgus Macaques ◽  
Immunogen Design ◽  
Humoral Responses ◽  
Hiv 1 ◽  
Vector Particles

Integrase Defective Lentiviral Vectors (IDLVs) represent an attractive vaccine platform for delivering HIV-1 antigens, given their ability to induce specific and persistent immune responses in both mice and non-human primates (NHPs). Recent advances in HIV-1 immunogen design demonstrated that native-like HIV-1 Envelope (Env) trimers that mimic the structure of virion-associated Env induce neutralization breadth in rabbits and macaques. Here, we describe the development of an IDLV-based HIV-1 vaccine expressing either soluble ConSOSL.UFO.664 or membrane-tethered ConSOSL.UFO.750 native-like Env immunogens with enhanced bNAb epitopes exposure. We show that IDLV can be pseudotyped with properly folded membrane-tethered native-like UFO.750 trimers. After a single IDLV injection in BALB/c mice, IDLV-UFO.750 induced a faster humoral kinetic as well as higher levels of anti-Env IgG compared to IDLV-UFO.664. IDLV-UFO.750 vaccinated cynomolgus macaques developed unusually long-lasting anti-Env IgG antibodies, as underlined by their remarkable half-life both after priming and boost with IDLV. After boosting with recombinant ConM SOSIP.v7 protein, two animals developed neutralization activity against the autologous tier 1B ConS virus mediated by V1/V2 and V3 glycan sites responses. By combining the possibility to display stabilized trimeric Env on the vector particles with the ability to induce sustained humoral responses, IDLVs represent an appropriate strategy for delivering rationally designed antigens to progress towards an effective HIV-1 vaccine.

A comparative evaluation of nasal and parenteral vaccine adjuvants to elicit systemic and mucosal HIV-1 peptide-specific humoral immune responses in cynomolgus macaques

Vaccine ◽  
2004 ◽  
Vol 22 (27-28) ◽  
pp. 3774-3788 ◽  
Author(s):  
Michael A Egan ◽  
Siew Yen Chong ◽  
Michael Hagen ◽  
Shakuntala Megati ◽  
Eva B Schadeck ◽  
...  
Keyword(s):  
Immune Responses ◽  
Comparative Evaluation ◽  
Vaccine Adjuvants ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Cynomolgus Macaques ◽  
Hiv 1

scholarly journals VDJ Gene Usage in IgM Repertoires of Rhesus and Cynomolgus Macaques

2022 ◽  
Vol 12 ◽  
Author(s):  
Mark Chernyshev ◽  
Mateusz Kaduk ◽  
Martin Corcoran ◽  
Gunilla B. Karlsson Hedestam
Keyword(s):  
Immune Responses ◽  
Rhesus Macaques ◽  
Binding Mode ◽  
Antibody Responses ◽  
Gene Frequencies ◽  
Vdj Recombination ◽  
Chinese Origin ◽  
Cynomolgus Macaques ◽  
Gene Usage ◽  
Hiv 1

Macaques are frequently used to evaluate candidate vaccines and to study infection-induced antibody responses, requiring an improved understanding of their naïve immunoglobulin (IG) repertoires. Baseline gene usage frequencies contextualize studies of antigen-specific immune responses, providing information about how easily one may stimulate a response with a particular VDJ recombination. Studies of human IgM repertoires have shown that IG VDJ gene frequencies vary several orders of magnitude between the most and least utilized genes in a manner that is consistent across many individuals but to date similar analyses are lacking for macaque IgM repertoires. Here, we quantified VDJ gene usage levels in unmutated IgM repertoires of 45 macaques, belonging to two species and four commonly used subgroups: Indian and Chinese origin rhesus macaques and Indonesian and Mauritian origin cynomolgus macaques. We show that VDJ gene frequencies differed greatly between the most and least used genes, with similar overall patterns observed in macaque subgroups and individuals. However, there were also clear differences affecting the use of specific V, D and J genes. Furthermore, in contrast to humans, macaques of both species utilized IGHV4 family genes to a much higher extent and showed evidence of evolutionary expansion of genes of this family. Finally, we used the results to inform the analysis of a broadly neutralizing HIV-1 antibody elicited in SHIV-infected rhesus macaques, RHA1.V2.01, which binds the apex of the Env trimer in a manner that mimics the binding mode of PGT145. We discuss the likelihood that similar antibodies could be elicited in different macaque subgroups.

scholarly journals Development of Th1 Imprints to rBCG Expressing a Foreign Protein: Implications for Vaccination against HIV-1 and Diverse Influenza Strains

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Carl Power ◽  
Travis W. Marfleet ◽  
Louis Qualtiere ◽  
Wei Xiao ◽  
Peter Bretscher
Keyword(s):  
Immune Responses ◽  
Foreign Protein ◽  
Th2 Response ◽  
Bacille Calmette Guerin ◽  
Th1 Response ◽  
Vaccination Strategies ◽  
Hiv 1

We demonstrate here that immunizing naïve mice with low numbers of recombinant Bacille Calmette-Guérin (rBCG) expressingβ-galactosidase (β-gal) generates predominant Th1 responses to both BCG andβ-gal whereas infection with high numbers generates a mixed Th1/Th2 response to both BCG andβ-gal. Furthermore, the Th1 response to both BCG andβ-gal is stable when mice, pre-exposed to low numbers of rBCG, are challenged four months later with high numbers of rBCG. Thus the Th1/Th2 phenotypes of the immune responses toβ-gal and to BCG are “coherently” regulated. Such rBCG vectors, encoding antigens of pathogens preferentially susceptible to cell-mediated attack, may be useful in vaccinating against such pathogens. We discuss vaccination strategies employing rBCG vectors that are designed to provide protection against diverse influenza strains or numerous variants of HIV-1 and consider what further experiments are essential to explore the possibility of realizing such strategies.

scholarly journals Immunogenicity and protective efficacy of enterotoxigenic Escherichia coli (ETEC) total RNA against ETEC challenge in a mouse model

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mandi Liu ◽  
Yue Zhang ◽  
Di Zhang ◽  
Yun Bai ◽  
Guomei Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Mouse Model ◽  
Immune Responses ◽  
Protective Efficacy ◽  
Enterotoxigenic Escherichia Coli ◽  
Economic Losses ◽  
Th2 Response ◽  
Total Rna ◽  
Etec Infection

AbstractEnterotoxigenic Escherichia coli (ETEC), an essential cause of post-weaning diarrhea (PWD) in piglets, leads to significant economic losses to the pig industry. The present study aims to identify the role of ETEC total RNA in eliciting immune responses to protect animals against ETEC infection. The results showed that the total RNA isolated from pig-derived ETEC K88ac strain effectively stimulated the IL-1β secretion of porcine intestinal epithelial cells (IPEC-J2). The mouse model immunized with ETEC total RNA via intramuscular injection (IM) or oral route (OR) was used to evaluate the protective efficiency of the ETEC total RNA. The results suggested that 70 μg ETEC total RNA administered by either route significantly promoted the production of the serum IL-1β and K88ac specific immunoglobulins (IgG, IgM, and IgA). Besides, the ETEC RNA administration augmented strong mucosal immunity by elevating K88ac specific IgA level in the intestinal fluid. Intramuscularly administered RNA induced a Th1/Th2 shift toward a Th2 response, while the orally administered RNA did not. The ETEC total RNA efficiently protected the animals against the ETEC challenge either by itself or as an adjuvant. The histology characterization of the small intestines also suggested the ETEC RNA administration protected the small intestinal structure against the ETEC infection. Particularly of note was that the immunity level and protective efficacy caused by ETEC RNA were dose-dependent. These findings will help understand the role of bacterial RNA in eliciting immune responses, and benefit the development of RNA-based vaccines or adjuvants.

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