Johne's disease in cattle is associated with enhanced expression of genes encoding IL-5, GATA-3, tissue inhibitors of matrix metalloproteinases 1 and 2, and factors promoting apoptosis in peripheral blood mononuclear cells

2005 ◽  
Vol 105 (3-4) ◽  
pp. 221-234 ◽  
Author(s):  
Paul M. Coussens ◽  
Chas B. Pudrith ◽  
Kerstin Skovgaard ◽  
Xiaoning Ren ◽  
Steven P. Suchyta ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Tissue Inhibitors ◽  
Expression Of Genes ◽  
Blood Mononuclear Cells ◽  
Genes Encoding ◽  
Enhanced Expression

scholarly journals Trypanosoma cruziSSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Yadira Morán-Utrera ◽  
Aracely López-Monteon ◽  
José Luis Rosales-Encina ◽  
Enrique Méndez-Bolaina ◽  
Angel Ramos-Ligonio
Keyword(s):  
Chagas Disease ◽  
Acute Phase ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
No Production ◽  
Peripheral Blood Mononuclear ◽  
Expression Of Genes ◽  
Blood Mononuclear Cells ◽  
Host Parasite

The acute phase of Chagas' disease in mice and human is marked by states of immunosuppression, in whichTrypanosoma cruzireplicates extensively and releases immunomodulatory molecules that delay parasite-specific responses mediated by effector T cells. This mechanism of evasion allows the parasite to spread in the host. Parasite molecules that regulate the host immune response during Chagas’ disease have not been fully identified, particularly proteins of the amastigote stage. In this work, we evaluated the role of the GPI anchored SSP4 protein ofT. cruzias an immunomodulatory molecule in peripheral blood mononuclear cells (PBMCs). rMBP::SSP4 protein was able to stimulate nitric oxide (NO) production. Likewise, rMBP::SSP4 induced the expression of genes and production of molecules involved in the inflammatory process, such as, cytokines, chemokines, and adhesion molecules (CAMs) as determined by RT-PCR and ELISA. These results suggest that the amastigote SSP4 molecule could play a key role in the immunoregulatory and/or immunosuppressive process observed in the acute phase of infection withT. cruzi.

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